Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_2744 and PP_2745 are separated by 95 nucleotides PP_2745 and PP_2746 are separated by 337 nucleotides
PP_2744: PP_2744 - Ribose-phosphate pyrophosphokinase family protein, at 3,126,465 to 3,127,424
_2744
PP_2745: PP_2745 - Universal stress protein family, at 3,127,520 to 3,128,416
_2745
PP_2746: PP_2746 - dimethyl-sulfide monooxygenase, at 3,128,754 to 3,130,163
_2746
Position (kb) 3127
3128
3129 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3126.955 kb on - strand, within PP_2744 at 3127.110 kb on - strand, within PP_2744 at 3127.110 kb on - strand, within PP_2744 at 3127.110 kb on - strand, within PP_2744 at 3127.110 kb on - strand, within PP_2744 at 3127.110 kb on - strand, within PP_2744 at 3127.110 kb on - strand, within PP_2744 at 3127.126 kb on + strand, within PP_2744 at 3127.127 kb on - strand, within PP_2744 at 3127.136 kb on - strand, within PP_2744 at 3127.206 kb on + strand, within PP_2744 at 3127.206 kb on + strand, within PP_2744 at 3127.206 kb on + strand, within PP_2744 at 3127.207 kb on - strand, within PP_2744 at 3127.207 kb on - strand, within PP_2744 at 3127.241 kb on - strand, within PP_2744 at 3127.375 kb on + strand at 3127.488 kb on + strand at 3127.492 kb on + strand at 3127.573 kb on + strand at 3127.573 kb on + strand at 3127.573 kb on + strand at 3127.609 kb on - strand at 3127.654 kb on - strand, within PP_2745 at 3127.807 kb on - strand, within PP_2745 at 3127.833 kb on + strand, within PP_2745 at 3127.955 kb on + strand, within PP_2745 at 3128.019 kb on - strand, within PP_2745 at 3128.410 kb on + strand at 3128.410 kb on + strand at 3128.411 kb on - strand at 3128.411 kb on - strand at 3128.500 kb on + strand at 3128.501 kb on - strand at 3128.504 kb on + strand at 3128.529 kb on - strand at 3128.592 kb on + strand at 3128.592 kb on + strand at 3128.673 kb on + strand at 3128.850 kb on + strand at 3128.940 kb on + strand, within PP_2746 at 3128.941 kb on - strand, within PP_2746 at 3128.941 kb on - strand, within PP_2746 at 3129.158 kb on + strand, within PP_2746 at 3129.158 kb on + strand, within PP_2746 at 3129.158 kb on + strand, within PP_2746 at 3129.166 kb on - strand, within PP_2746 at 3129.231 kb on + strand, within PP_2746 at 3129.244 kb on - strand, within PP_2746 at 3129.258 kb on + strand, within PP_2746 at 3129.259 kb on - strand, within PP_2746
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 3,126,955 - PP_2744 0.51 -1.0 3,127,110 - PP_2744 0.67 -0.8 3,127,110 - PP_2744 0.67 -0.4 3,127,110 - PP_2744 0.67 -1.6 3,127,110 - PP_2744 0.67 +0.7 3,127,110 - PP_2744 0.67 -0.4 3,127,110 - PP_2744 0.67 -0.1 3,127,126 + PP_2744 0.69 +0.2 3,127,127 - PP_2744 0.69 -0.8 3,127,136 - PP_2744 0.70 -1.0 3,127,206 + PP_2744 0.77 +0.0 3,127,206 + PP_2744 0.77 +0.5 3,127,206 + PP_2744 0.77 +0.1 3,127,207 - PP_2744 0.77 -0.1 3,127,207 - PP_2744 0.77 -1.0 3,127,241 - PP_2744 0.81 -0.2 3,127,375 + -0.4 3,127,488 + -0.1 3,127,492 + +0.0 3,127,573 + -0.1 3,127,573 + -0.4 3,127,573 + +2.7 3,127,609 - -0.7 3,127,654 - PP_2745 0.15 -3.4 3,127,807 - PP_2745 0.32 -1.7 3,127,833 + PP_2745 0.35 -0.4 3,127,955 + PP_2745 0.48 -0.4 3,128,019 - PP_2745 0.56 -0.9 3,128,410 + -1.0 3,128,410 + +0.7 3,128,411 - +0.4 3,128,411 - +0.9 3,128,500 + -0.2 3,128,501 - +3.7 3,128,504 + +0.2 3,128,529 - +0.9 3,128,592 + -0.9 3,128,592 + +0.0 3,128,673 + +0.4 3,128,850 + -1.2 3,128,940 + PP_2746 0.13 -0.1 3,128,941 - PP_2746 0.13 +0.9 3,128,941 - PP_2746 0.13 +1.4 3,129,158 + PP_2746 0.29 -1.3 3,129,158 + PP_2746 0.29 -0.3 3,129,158 + PP_2746 0.29 +0.7 3,129,166 - PP_2746 0.29 +1.4 3,129,231 + PP_2746 0.34 +0.4 3,129,244 - PP_2746 0.35 -0.0 3,129,258 + PP_2746 0.36 -0.3 3,129,259 - PP_2746 0.36 +0.6
Or see this region's nucleotide sequence