Strain Fitness in Pseudomonas putida KT2440 around PP_2348

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,677,024	-				-0.5
2,677,024	-				-1.0
2,677,068	-		PP_2347	0.11	+0.0
2,677,107	-		PP_2347	0.17	-1.0
2,677,218	+		PP_2347	0.33	-0.6
2,677,287	-		PP_2347	0.44	-0.4
2,677,287	-		PP_2347	0.44	-0.2
2,677,475	+		PP_2347	0.72	+0.7
2,677,532	+		PP_2347	0.80	+1.0
2,677,533	-		PP_2347	0.80	+0.1
2,677,533	-		PP_2347	0.80	+0.1
2,677,637	+				+0.6
2,677,637	+				+0.7
2,677,637	+				-0.2
2,677,637	+				+0.2
2,677,638	-				-1.3
2,677,638	-				-0.7
2,677,638	-				-0.0
2,677,638	-				+0.2
2,677,643	+				-0.8
2,677,643	+				+0.3
2,677,644	-				-1.3
2,677,644	-				-1.2
2,677,644	-				+1.1
2,677,644	-				+2.0
2,677,703	+				-1.4
2,677,873	+		PP_2348	0.16	-3.4
2,677,897	+		PP_2348	0.18	-2.0
2,677,898	-		PP_2348	0.18	-2.2
2,677,898	-		PP_2348	0.18	-2.3
2,678,086	+		PP_2348	0.32	-2.7
2,678,087	-		PP_2348	0.32	-2.6
2,678,087	-		PP_2348	0.32	-0.4
2,678,289	-		PP_2348	0.48	-1.5
2,678,319	+		PP_2348	0.50	-2.0
2,678,319	+		PP_2348	0.50	-3.6
2,679,030	-				+0.7
2,679,030	-				-0.5
2,679,049	+				+0.7
2,679,056	-				+0.8
2,679,056	-				+0.2
2,679,056	-				+1.5
2,679,177	+				-0.4
2,679,178	-				-0.5
2,679,398	+		PP_2349	0.12	+1.0
2,679,399	-		PP_2349	0.12	+0.2
2,679,451	-		PP_2349	0.17	-0.4
2,679,509	+		PP_2349	0.22	-0.1
2,679,509	+		PP_2349	0.22	-0.7
2,679,510	-		PP_2349	0.22	+0.6
2,679,753	-		PP_2349	0.43	-0.3
2,679,753	-		PP_2349	0.43	-0.6
2,679,753	-		PP_2349	0.43	-0.2
2,679,753	-		PP_2349	0.43	-1.7