Strain Fitness in Pseudomonas putida KT2440 around PP_2263

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,580,747	+				+0.1
2,580,748	-				+0.5
2,580,748	-				-0.6
2,580,748	-				-0.6
2,580,748	-				-0.1
2,580,748	-				+0.1
2,580,748	-				-1.1
2,580,918	-		PP_2262	0.16	-0.4
2,580,995	+				+1.3
2,580,995	+		PP_2262	0.25	+0.2
2,581,035	+		PP_2262	0.30	+0.7
2,581,035	+		PP_2262	0.30	+0.9
2,581,035	+		PP_2262	0.30	-1.1
2,581,035	+		PP_2262	0.30	-0.8
2,581,036	-		PP_2262	0.30	+0.6
2,581,036	-		PP_2262	0.30	-0.1
2,581,036	-		PP_2262	0.30	-1.0
2,581,036	-		PP_2262	0.30	-0.3
2,581,136	-		PP_2262	0.41	-0.5
2,581,144	+		PP_2262	0.42	-0.3
2,581,205	+		PP_2262	0.49	+0.0
2,581,205	+		PP_2262	0.49	+0.1
2,581,205	+		PP_2262	0.49	+0.5
2,581,205	+		PP_2262	0.49	-0.4
2,581,212	-		PP_2262	0.50	-0.0
2,581,295	+		PP_2262	0.60	+0.4
2,581,296	-		PP_2262	0.60	-0.6
2,581,325	+		PP_2262	0.63	-0.0
2,581,326	-		PP_2262	0.63	+0.2
2,581,326	-		PP_2262	0.63	+0.6
2,581,329	-		PP_2262	0.63	-0.9
2,581,451	+		PP_2262	0.78	+0.4
2,581,452	-		PP_2262	0.78	-0.2
2,581,526	-		PP_2262	0.86	+2.2
2,581,526	-		PP_2262	0.86	-0.6
2,581,577	+				-0.9
2,581,578	-				-1.5
2,581,643	+				-0.6
2,581,643	+				+0.6
2,581,643	+				+0.6
2,581,643	+				+1.0
2,581,683	-				+0.4
2,581,710	-				-0.3
2,581,796	+		PP_2263	0.18	-0.6
2,581,835	+		PP_2263	0.23	-2.5
2,581,835	+		PP_2263	0.23	-1.4
2,581,835	+		PP_2263	0.23	+1.2
2,581,851	-		PP_2263	0.25	-0.2
2,581,851	-		PP_2263	0.25	+0.4
2,581,851	-		PP_2263	0.25	+0.1
2,581,851	-		PP_2263	0.25	+1.3
2,581,851	-		PP_2263	0.25	-0.1
2,581,851	-		PP_2263	0.25	+0.0
2,581,853	+		PP_2263	0.25	-0.2
2,581,853	+		PP_2263	0.25	+0.3
2,581,853	+		PP_2263	0.25	-0.6
2,581,853	+		PP_2263	0.25	+0.1
2,581,853	+		PP_2263	0.25	+1.0
2,581,853	+		PP_2263	0.25	+0.7
2,581,854	-		PP_2263	0.26	-0.3
2,581,854	-		PP_2263	0.26	-0.4
2,581,854	-		PP_2263	0.26	+0.4
2,581,854	-		PP_2263	0.26	+0.7
2,581,854	-		PP_2263	0.26	-2.3
2,581,854	-		PP_2263	0.26	-0.1
2,581,854	-		PP_2263	0.26	+0.1
2,581,904	+		PP_2263	0.32	-0.8
2,581,904	+		PP_2263	0.32	-0.4
2,581,905	-		PP_2263	0.32	-0.7
2,581,905	-		PP_2263	0.32	+1.2
2,582,012	+		PP_2263	0.45	-0.8
2,582,012	+		PP_2263	0.45	+1.1
2,582,013	-		PP_2263	0.45	+0.6
2,582,157	-		PP_2263	0.63	+0.2
2,582,563	-		PP_5505	0.37	-0.1
2,582,637	-		PP_5505	0.64	-1.5
2,582,637	-		PP_5505	0.64	-1.2
2,582,637	-		PP_5505	0.64	-1.8
2,582,826	+				+0.6
2,582,827	-				+0.6
2,582,865	-				+1.4
2,583,023	+		PP_2264	0.14	-0.1
2,583,149	+		PP_2264	0.22	-0.6
2,583,149	+		PP_2264	0.22	-0.7
2,583,149	+		PP_2264	0.22	-1.7
2,583,149	+		PP_2264	0.22	-0.5
2,583,150	-		PP_2264	0.22	-0.4
2,583,150	-		PP_2264	0.22	+3.0
2,583,250	+		PP_2264	0.27	-0.0
2,583,251	-		PP_2264	0.27	-2.1
2,583,320	+		PP_2264	0.31	-0.5
2,583,320	+		PP_2264	0.31	+0.7
2,583,320	+		PP_2264	0.31	-0.0
2,583,353	+		PP_2264	0.33	-0.8
2,583,413	+		PP_2264	0.37	-0.6
2,583,413	+		PP_2264	0.37	-0.5
2,583,413	+		PP_2264	0.37	+0.8
2,583,413	+		PP_2264	0.37	-0.6
2,583,413	+		PP_2264	0.37	-0.6
2,583,414	-		PP_2264	0.37	-0.7
2,583,414	-		PP_2264	0.37	+0.8