Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1782 and PP_1783 overlap by 1 nucleotides PP_1783 and PP_1784 are separated by 11 nucleotides PP_1784 and PP_1785 overlap by 4 nucleotides
PP_1782: PP_1782 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 1,996,039 to 1,996,587
_1782
PP_1783: PP_1783 - dTDP-glucose pyrophosphorylase, at 1,996,587 to 1,997,468
_1783
PP_1784: PP_1784 - dTDP-4-dehydrorhamnose reductase, at 1,997,480 to 1,998,382
_1784
PP_1785: PP_1785 - dTDP-glucose 4,6-dehydratase 2, at 1,998,379 to 1,999,479
_1785
Position (kb)
1996
1997
1998 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1995.608 kb on + strand at 1995.623 kb on + strand at 1995.623 kb on + strand at 1995.623 kb on + strand at 1995.623 kb on + strand at 1995.623 kb on + strand at 1995.623 kb on + strand at 1995.624 kb on - strand at 1995.624 kb on - strand at 1995.624 kb on - strand at 1995.684 kb on + strand at 1995.684 kb on + strand at 1995.685 kb on - strand at 1995.685 kb on - strand at 1995.685 kb on - strand at 1995.742 kb on + strand at 1995.742 kb on + strand at 1995.753 kb on + strand at 1995.754 kb on - strand at 1995.759 kb on + strand at 1995.759 kb on + strand at 1995.759 kb on + strand at 1995.760 kb on - strand at 1995.760 kb on - strand at 1995.827 kb on + strand at 1995.827 kb on + strand at 1995.827 kb on + strand at 1995.827 kb on + strand at 1995.828 kb on - strand at 1995.828 kb on - strand at 1995.828 kb on - strand at 1995.841 kb on - strand at 1995.841 kb on - strand at 1995.871 kb on + strand at 1995.890 kb on - strand at 1995.943 kb on + strand at 1995.943 kb on + strand at 1996.031 kb on + strand at 1996.175 kb on + strand, within PP_1782 at 1996.176 kb on - strand, within PP_1782 at 1996.193 kb on + strand, within PP_1782 at 1996.193 kb on + strand, within PP_1782 at 1996.291 kb on + strand, within PP_1782 at 1996.299 kb on + strand, within PP_1782 at 1996.300 kb on - strand, within PP_1782 at 1996.400 kb on + strand, within PP_1782 at 1996.401 kb on - strand, within PP_1782 at 1996.401 kb on - strand, within PP_1782 at 1996.450 kb on + strand, within PP_1782 at 1996.451 kb on - strand, within PP_1782 at 1996.451 kb on - strand, within PP_1782 at 1996.508 kb on + strand, within PP_1782 at 1996.509 kb on - strand, within PP_1782 at 1996.567 kb on - strand at 1996.757 kb on - strand at 1996.757 kb on - strand, within PP_1783 at 1996.757 kb on - strand, within PP_1783 at 1996.757 kb on - strand at 1996.757 kb on - strand at 1996.808 kb on - strand, within PP_1783 at 1996.853 kb on - strand at 1996.853 kb on - strand, within PP_1783 at 1996.961 kb on - strand, within PP_1783 at 1996.961 kb on - strand, within PP_1783 at 1996.961 kb on - strand at 1997.060 kb on - strand at 1997.136 kb on - strand, within PP_1783 at 1997.234 kb on - strand at 1997.479 kb on - strand at 1997.482 kb on - strand at 1998.388 kb on - strand at 1998.388 kb on - strand at 1998.398 kb on + strand at 1998.398 kb on + strand at 1998.398 kb on + strand at 1998.399 kb on - strand at 1998.422 kb on + strand at 1998.423 kb on - strand at 1998.446 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,995,608 + -0.4 1,995,623 + -0.3 1,995,623 + +0.8 1,995,623 + -0.2 1,995,623 + -1.4 1,995,623 + +0.3 1,995,623 + +1.1 1,995,624 - +1.7 1,995,624 - +0.5 1,995,624 - +0.4 1,995,684 + -0.9 1,995,684 + +0.3 1,995,685 - -0.1 1,995,685 - +3.0 1,995,685 - -1.1 1,995,742 + +0.7 1,995,742 + -0.4 1,995,753 + -1.3 1,995,754 - -0.4 1,995,759 + -0.9 1,995,759 + -1.2 1,995,759 + -1.2 1,995,760 - -2.2 1,995,760 - -0.4 1,995,827 + +0.8 1,995,827 + -1.3 1,995,827 + -1.8 1,995,827 + -0.4 1,995,828 - -0.7 1,995,828 - -0.9 1,995,828 - -0.3 1,995,841 - +0.7 1,995,841 - -0.7 1,995,871 + +0.1 1,995,890 - -0.2 1,995,943 + -0.5 1,995,943 + +1.3 1,996,031 + +1.1 1,996,175 + PP_1782 0.25 -0.6 1,996,176 - PP_1782 0.25 -1.5 1,996,193 + PP_1782 0.28 -0.3 1,996,193 + PP_1782 0.28 -0.3 1,996,291 + PP_1782 0.46 +0.1 1,996,299 + PP_1782 0.47 -0.6 1,996,300 - PP_1782 0.48 -1.2 1,996,400 + PP_1782 0.66 -0.2 1,996,401 - PP_1782 0.66 +2.1 1,996,401 - PP_1782 0.66 +0.0 1,996,450 + PP_1782 0.75 +0.2 1,996,451 - PP_1782 0.75 +0.6 1,996,451 - PP_1782 0.75 -1.0 1,996,508 + PP_1782 0.85 +0.5 1,996,509 - PP_1782 0.86 -1.3 1,996,567 - +0.2 1,996,757 - +2.7 1,996,757 - PP_1783 0.19 +3.7 1,996,757 - PP_1783 0.19 +3.7 1,996,757 - +3.7 1,996,757 - +3.7 1,996,808 - PP_1783 0.25 +3.7 1,996,853 - +3.7 1,996,853 - PP_1783 0.30 +2.1 1,996,961 - PP_1783 0.42 +3.7 1,996,961 - PP_1783 0.42 +3.7 1,996,961 - +3.7 1,997,060 - +2.1 1,997,136 - PP_1783 0.62 +2.1 1,997,234 - +2.7 1,997,479 - -0.4 1,997,482 - -0.4 1,998,388 - +0.5 1,998,388 - -0.6 1,998,398 + -2.1 1,998,398 + -0.4 1,998,398 + -0.7 1,998,399 - +1.7 1,998,422 + +0.9 1,998,423 - +1.6 1,998,446 - +0.3
Or see this region's nucleotide sequence