Strain Fitness in Pseudomonas putida KT2440 around PP_1300

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1299 and PP_1300 are separated by 10 nucleotidesPP_1300 and PP_1301 are separated by 129 nucleotides PP_1299: PP_1299 - putative amino acid ABC transporter - membrane subunit, at 1,485,112 to 1,486,209 _1299 PP_1300: PP_1300 - putative amino acid ABC transporter - ATP-binding subunit, at 1,486,220 to 1,486,984 _1300 PP_1301: PP_1301 - quality control serine endoprotease DegS, at 1,487,114 to 1,488,322 _1301 Position (kb) 1486 1487Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1485.235 kb on + strand, within PP_1299at 1485.235 kb on + strand, within PP_1299at 1485.236 kb on - strand, within PP_1299at 1485.236 kb on - strand, within PP_1299at 1485.373 kb on + strand, within PP_1299at 1485.373 kb on + strand, within PP_1299at 1485.373 kb on + strand, within PP_1299at 1485.374 kb on - strand, within PP_1299at 1485.379 kb on + strand, within PP_1299at 1485.379 kb on + strand, within PP_1299at 1485.379 kb on + strand, within PP_1299at 1485.379 kb on + strand, within PP_1299at 1485.380 kb on - strand, within PP_1299at 1485.542 kb on - strand, within PP_1299at 1485.737 kb on + strand, within PP_1299at 1485.738 kb on - strand, within PP_1299at 1485.892 kb on + strand, within PP_1299at 1485.892 kb on + strand, within PP_1299at 1485.893 kb on - strand, within PP_1299at 1485.923 kb on - strand, within PP_1299at 1486.111 kb on + strandat 1486.111 kb on + strandat 1486.111 kb on + strandat 1486.112 kb on - strandat 1486.152 kb on + strandat 1486.153 kb on - strandat 1486.153 kb on - strandat 1486.163 kb on - strandat 1486.163 kb on - strandat 1486.289 kb on + strandat 1486.617 kb on - strand, within PP_1300at 1486.617 kb on - strand, within PP_1300at 1486.655 kb on + strand, within PP_1300at 1486.656 kb on - strand, within PP_1300at 1486.656 kb on - strand, within PP_1300at 1486.790 kb on - strand, within PP_1300at 1487.039 kb on - strandat 1487.062 kb on - strandat 1487.115 kb on + strandat 1487.116 kb on - strandat 1487.548 kb on + strand, within PP_1301at 1487.548 kb on + strand, within PP_1301at 1487.549 kb on - strandat 1487.640 kb on + strand, within PP_1301at 1487.640 kb on + strand, within PP_1301at 1487.640 kb on + strand, within PP_1301at 1487.640 kb on + strand, within PP_1301at 1487.640 kb on + strand, within PP_1301at 1487.641 kb on - strand, within PP_1301at 1487.876 kb on + strand, within PP_1301at 1487.925 kb on + strand, within PP_1301at 1487.925 kb on + strand, within PP_1301at 1487.926 kb on - strand, within PP_1301

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,485,235 + PP_1299 0.11 -0.0
1,485,235 + PP_1299 0.11 +0.3
1,485,236 - PP_1299 0.11 +0.7
1,485,236 - PP_1299 0.11 -2.1
1,485,373 + PP_1299 0.24 -1.2
1,485,373 + PP_1299 0.24 -1.4
1,485,373 + PP_1299 0.24 -0.1
1,485,374 - PP_1299 0.24 -0.3
1,485,379 + PP_1299 0.24 -0.1
1,485,379 + PP_1299 0.24 +0.2
1,485,379 + PP_1299 0.24 -1.2
1,485,379 + PP_1299 0.24 -1.3
1,485,380 - PP_1299 0.24 +0.2
1,485,542 - PP_1299 0.39 -0.5
1,485,737 + PP_1299 0.57 -0.5
1,485,738 - PP_1299 0.57 -0.0
1,485,892 + PP_1299 0.71 -0.4
1,485,892 + PP_1299 0.71 -1.1
1,485,893 - PP_1299 0.71 -0.3
1,485,923 - PP_1299 0.74 +1.2
1,486,111 + -1.1
1,486,111 + +0.7
1,486,111 + +1.3
1,486,112 - -0.8
1,486,152 + -1.8
1,486,153 - +0.5
1,486,153 - -0.6
1,486,163 - -0.5
1,486,163 - -2.3
1,486,289 + -0.9
1,486,617 - PP_1300 0.52 -0.8
1,486,617 - PP_1300 0.52 +1.2
1,486,655 + PP_1300 0.57 -1.0
1,486,656 - PP_1300 0.57 -2.6
1,486,656 - PP_1300 0.57 +0.1
1,486,790 - PP_1300 0.75 -0.6
1,487,039 - -0.4
1,487,062 - -0.7
1,487,115 + +0.7
1,487,116 - +2.2
1,487,548 + PP_1301 0.36 +0.1
1,487,548 + PP_1301 0.36 -2.5
1,487,549 - +3.7
1,487,640 + PP_1301 0.44 +0.8
1,487,640 + PP_1301 0.44 -0.5
1,487,640 + PP_1301 0.44 -0.7
1,487,640 + PP_1301 0.44 -0.7
1,487,640 + PP_1301 0.44 -0.2
1,487,641 - PP_1301 0.44 +0.2
1,487,876 + PP_1301 0.63 -0.9
1,487,925 + PP_1301 0.67 -0.6
1,487,925 + PP_1301 0.67 -1.2
1,487,926 - PP_1301 0.67 -0.6

Or see this region's nucleotide sequence