Strain Fitness in Pseudomonas putida KT2440 around PP_1288

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
1,472,188	+		PP_1287	0.57	-1.3
1,472,390	+		PP_1287	0.71	+0.0
1,472,498	+		PP_1287	0.78	-1.2
1,472,499	-		PP_1287	0.78	-1.0
1,472,499	-		PP_1287	0.78	-0.1
1,472,499	-		PP_1287	0.78	-1.1
1,472,526	+		PP_1287	0.80	-0.4
1,472,549	+		PP_1287	0.81	-0.7
1,472,549	+		PP_1287	0.81	-0.2
1,472,550	-		PP_1287	0.81	+0.2
1,472,575	-		PP_1287	0.83	-1.1
1,472,603	+		PP_1287	0.85	+0.1
1,472,603	+		PP_1287	0.85	-0.3
1,472,610	-		PP_1287	0.85	+0.5
1,472,618	+		PP_1287	0.86	-1.2
1,472,663	+		PP_1287	0.89	-0.7
1,472,664	-		PP_1287	0.89	+0.2
1,472,664	-		PP_1287	0.89	-0.1
1,472,724	+				+0.4
1,472,724	+				+0.7
1,472,768	+				-0.9
1,472,768	+				-1.2
1,472,769	-				+0.3
1,472,769	-				-1.0
1,472,905	+				-1.7
1,473,118	+		PP_1288	0.16	-0.7
1,473,118	+		PP_1288	0.16	-0.6
1,473,118	+		PP_1288	0.16	+0.3
1,473,166	+		PP_1288	0.20	+0.6
1,473,166	+		PP_1288	0.20	+0.1
1,473,167	-		PP_1288	0.20	-0.4
1,473,167	-				+2.7
1,473,167	-		PP_1288	0.20	-0.9
1,473,167	-		PP_1288	0.20	-1.1
1,473,402	+		PP_1288	0.38	-1.2
1,473,451	+		PP_1288	0.42	-0.8
1,473,451	+		PP_1288	0.42	-0.6
1,473,451	+		PP_1288	0.42	-0.5
1,473,451	+		PP_1288	0.42	-1.0
1,473,452	-		PP_1288	0.42	-1.8
1,473,452	-		PP_1288	0.42	-1.0
1,473,452	-		PP_1288	0.42	+0.1
1,473,452	-		PP_1288	0.42	+0.4
1,473,454	+		PP_1288	0.42	+0.2
1,473,589	+		PP_1288	0.52	-0.2
1,473,589	+		PP_1288	0.52	+0.4
1,473,589	+		PP_1288	0.52	-0.5
1,473,590	-		PP_1288	0.52	+1.3
1,473,590	-		PP_1288	0.52	+2.2
1,473,590	-		PP_1288	0.52	-0.5
1,473,590	-		PP_1288	0.52	-1.1
1,473,650	-		PP_1288	0.57	-1.2
1,473,650	-		PP_1288	0.57	+0.2
1,473,718	+		PP_1288	0.62	+1.1
1,473,718	+		PP_1288	0.62	-1.2
1,473,718	+		PP_1288	0.62	-0.3
1,473,718	+		PP_1288	0.62	-0.2
1,473,846	+		PP_1288	0.72	-0.1
1,473,847	-		PP_1288	0.72	-0.8
1,473,928	+		PP_1288	0.78	-1.0
1,473,929	-		PP_1288	0.78	-0.1
1,473,929	-		PP_1288	0.78	-0.7
1,474,481	+				-0.2
1,474,516	-				-0.3
1,474,616	+				-2.2
1,474,617	-				-1.1
1,474,637	+				-0.3
1,474,638	-				-0.3
1,474,638	-				-0.9
1,474,661	+				+2.2
1,474,661	+				+0.6
1,474,728	+				-0.3
1,474,785	-				-1.4
1,474,793	+				+0.7
1,474,793	+				-0.2
1,474,870	-				+2.2
1,475,082	+				+0.1
1,475,082	+				+1.7
1,475,082	+				-1.2
1,475,082	+				-0.4
1,475,082	+				+1.7
1,475,082	+				-0.0
1,475,082	+				+0.1
1,475,082	+				+0.4
1,475,082	+				+2.2
1,475,082	+				-0.5
1,475,082	+				+0.4
1,475,082	+				+0.9
1,475,082	+				-0.8
1,475,083	-				-1.1
1,475,083	-				+0.9
1,475,083	-				+0.5
1,475,083	-				-0.0
1,475,083	-				-1.1
1,475,083	-				-0.0
1,475,083	-				-2.0
1,475,085	+				-0.6
1,475,085	+				+1.4
1,475,085	+				+0.1
1,475,085	+				-1.2
1,475,085	+				-0.3
1,475,085	+				-0.7
1,475,086	-				+0.1
1,475,086	-				-0.6
1,475,086	-				-0.3
1,475,086	-				-1.1
1,475,086	-				+1.0
1,475,086	-				-1.6
1,475,086	-				+0.1
1,475,088	+				+0.2
1,475,181	+		PP_1289	0.21	+0.6
1,475,199	+		PP_1289	0.23	-0.1