Strain Fitness in Pseudomonas putida KT2440 around PP_1095

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1093 and PP_1094 overlap by 32 nucleotidesPP_1094 and PP_1095 overlap by 4 nucleotidesPP_1095 and PP_1096 overlap by 4 nucleotidesPP_1096 and PP_1097 are separated by 106 nucleotides PP_1093: PP_1093 - putative electron transport complex protein, at 1,250,722 to 1,251,270 _1093 PP_1094: PP_1094 - putative electron transport complex protein, at 1,251,239 to 1,251,841 _1094 PP_1095: PP_1095 - putative electron transport complex protein, at 1,251,838 to 1,252,809 _1095 PP_1096: PP_1096 - putative electron transport complex protein, at 1,252,806 to 1,253,354 _1096 PP_1097: PP_1097 - Methionine--tRNA ligase, at 1,253,461 to 1,255,500 _1097 Position (kb) 1251 1252 1253Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1250.941 kb on + strand, within PP_1093at 1250.942 kb on - strand, within PP_1093at 1250.988 kb on - strand, within PP_1093at 1251.082 kb on + strand, within PP_1093at 1251.083 kb on - strand, within PP_1093at 1251.083 kb on - strand, within PP_1093at 1251.083 kb on - strand, within PP_1093at 1251.086 kb on + strand, within PP_1093at 1251.086 kb on + strand, within PP_1093at 1251.086 kb on + strand, within PP_1093at 1251.087 kb on - strand, within PP_1093at 1251.087 kb on - strand, within PP_1093at 1251.087 kb on - strand, within PP_1093at 1251.087 kb on - strand, within PP_1093at 1251.087 kb on - strand, within PP_1093at 1251.109 kb on - strand, within PP_1093at 1251.109 kb on - strand, within PP_1093at 1251.151 kb on + strand, within PP_1093at 1251.240 kb on + strandat 1251.241 kb on - strandat 1251.241 kb on - strandat 1251.241 kb on - strandat 1251.241 kb on - strandat 1251.266 kb on + strandat 1251.266 kb on + strandat 1251.266 kb on + strandat 1251.267 kb on - strandat 1251.267 kb on - strandat 1251.267 kb on - strandat 1251.267 kb on - strandat 1251.271 kb on + strandat 1251.272 kb on - strandat 1251.272 kb on - strandat 1251.272 kb on - strandat 1251.272 kb on - strandat 1251.288 kb on + strandat 1251.288 kb on + strandat 1251.586 kb on + strand, within PP_1094at 1251.586 kb on + strand, within PP_1094at 1251.586 kb on + strand, within PP_1094at 1251.587 kb on - strand, within PP_1094at 1251.595 kb on + strand, within PP_1094at 1251.595 kb on + strand, within PP_1094at 1251.626 kb on - strand, within PP_1094at 1251.626 kb on - strand, within PP_1094at 1251.681 kb on + strand, within PP_1094at 1251.681 kb on + strand, within PP_1094at 1251.681 kb on + strand, within PP_1094at 1251.681 kb on + strand, within PP_1094at 1251.682 kb on - strand, within PP_1094at 1251.682 kb on - strand, within PP_1094at 1251.682 kb on - strand, within PP_1094at 1251.682 kb on - strand, within PP_1094at 1251.685 kb on + strand, within PP_1094at 1251.685 kb on + strand, within PP_1094at 1251.686 kb on - strand, within PP_1094at 1251.686 kb on - strand, within PP_1094at 1251.794 kb on + strandat 1251.794 kb on + strandat 1251.795 kb on - strandat 1251.795 kb on - strandat 1251.795 kb on - strandat 1251.957 kb on - strand, within PP_1095at 1251.957 kb on - strand, within PP_1095at 1251.957 kb on - strand, within PP_1095at 1252.132 kb on + strand, within PP_1095at 1252.132 kb on + strand, within PP_1095at 1252.189 kb on + strand, within PP_1095at 1252.189 kb on + strand, within PP_1095at 1252.190 kb on - strand, within PP_1095at 1252.454 kb on + strand, within PP_1095at 1252.454 kb on + strand, within PP_1095at 1252.455 kb on - strand, within PP_1095at 1252.499 kb on + strand, within PP_1095at 1252.500 kb on - strand, within PP_1095at 1252.500 kb on - strand, within PP_1095at 1252.500 kb on - strand, within PP_1095at 1252.500 kb on - strand, within PP_1095at 1252.589 kb on - strand, within PP_1095at 1252.618 kb on + strand, within PP_1095at 1252.844 kb on + strandat 1253.080 kb on - strand, within PP_1096at 1253.193 kb on + strand, within PP_1096at 1253.194 kb on - strand, within PP_1096at 1253.344 kb on + strandat 1253.344 kb on + strandat 1253.344 kb on + strandat 1253.414 kb on + strandat 1253.414 kb on + strandat 1253.463 kb on - strandat 1253.463 kb on - strandat 1253.463 kb on - strandat 1253.594 kb on - strandat 1253.636 kb on + strandat 1253.636 kb on + strandat 1253.772 kb on + strand, within PP_1097at 1253.773 kb on - strand, within PP_1097

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Per-strain Table

Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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1,250,941 + PP_1093 0.40 -0.5
1,250,942 - PP_1093 0.40 +0.3
1,250,988 - PP_1093 0.48 +0.1
1,251,082 + PP_1093 0.66 +0.2
1,251,083 - PP_1093 0.66 +0.9
1,251,083 - PP_1093 0.66 +1.4
1,251,083 - PP_1093 0.66 -0.1
1,251,086 + PP_1093 0.66 -1.3
1,251,086 + PP_1093 0.66 -1.0
1,251,086 + PP_1093 0.66 +0.9
1,251,087 - PP_1093 0.66 -1.0
1,251,087 - PP_1093 0.66 +1.6
1,251,087 - PP_1093 0.66 +0.0
1,251,087 - PP_1093 0.66 -0.0
1,251,087 - PP_1093 0.66 +1.6
1,251,109 - PP_1093 0.70 +0.5
1,251,109 - PP_1093 0.70 +0.7
1,251,151 + PP_1093 0.78 -1.6
1,251,240 + -0.3
1,251,241 - -0.1
1,251,241 - -2.1
1,251,241 - -0.5
1,251,241 - +1.0
1,251,266 + -1.1
1,251,266 + -0.8
1,251,266 + -1.5
1,251,267 - -0.9
1,251,267 - -1.2
1,251,267 - -0.6
1,251,267 - +1.7
1,251,271 + +0.6
1,251,272 - +0.3
1,251,272 - +0.0
1,251,272 - -0.7
1,251,272 - -0.2
1,251,288 + -1.0
1,251,288 + -0.3
1,251,586 + PP_1094 0.58 +0.1
1,251,586 + PP_1094 0.58 -0.0
1,251,586 + PP_1094 0.58 +0.2
1,251,587 - PP_1094 0.58 +0.2
1,251,595 + PP_1094 0.59 -1.1
1,251,595 + PP_1094 0.59 -1.1
1,251,626 - PP_1094 0.64 -0.4
1,251,626 - PP_1094 0.64 -0.9
1,251,681 + PP_1094 0.73 +0.1
1,251,681 + PP_1094 0.73 -0.6
1,251,681 + PP_1094 0.73 +0.3
1,251,681 + PP_1094 0.73 -1.2
1,251,682 - PP_1094 0.73 -0.3
1,251,682 - PP_1094 0.73 -0.1
1,251,682 - PP_1094 0.73 +0.1
1,251,682 - PP_1094 0.73 +0.6
1,251,685 + PP_1094 0.74 +1.2
1,251,685 + PP_1094 0.74 -0.9
1,251,686 - PP_1094 0.74 +0.5
1,251,686 - PP_1094 0.74 -0.2
1,251,794 + +0.4
1,251,794 + +1.5
1,251,795 - -0.1
1,251,795 - -0.6
1,251,795 - -1.9
1,251,957 - PP_1095 0.12 -2.8
1,251,957 - PP_1095 0.12 +0.7
1,251,957 - PP_1095 0.12 -1.7
1,252,132 + PP_1095 0.30 +0.0
1,252,132 + PP_1095 0.30 +0.3
1,252,189 + PP_1095 0.36 -0.5
1,252,189 + PP_1095 0.36 -1.7
1,252,190 - PP_1095 0.36 -0.5
1,252,454 + PP_1095 0.63 -2.2
1,252,454 + PP_1095 0.63 -0.5
1,252,455 - PP_1095 0.63 +0.6
1,252,499 + PP_1095 0.68 -1.7
1,252,500 - PP_1095 0.68 +0.2
1,252,500 - PP_1095 0.68 +1.2
1,252,500 - PP_1095 0.68 -0.0
1,252,500 - PP_1095 0.68 -1.2
1,252,589 - PP_1095 0.77 -1.2
1,252,618 + PP_1095 0.80 +1.7
1,252,844 + +0.3
1,253,080 - PP_1096 0.50 -0.2
1,253,193 + PP_1096 0.70 -0.5
1,253,194 - PP_1096 0.71 +0.0
1,253,344 + +0.4
1,253,344 + +0.6
1,253,344 + -0.4
1,253,414 + -0.3
1,253,414 + +0.0
1,253,463 - -0.9
1,253,463 - +0.1
1,253,463 - -0.6
1,253,594 - -1.2
1,253,636 + -1.4
1,253,636 + +3.7
1,253,772 + PP_1097 0.15 -0.6
1,253,773 - PP_1097 0.15 +0.9

Or see this region's nucleotide sequence