Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0792 and PP_0793 are separated by 284 nucleotides PP_0793 and PP_0794 are separated by 0 nucleotides PP_0794 and PP_0795 overlap by 4 nucleotides
PP_0792: PP_0792 - catabolite repressor-activator, DNA-binding transcriptional dual regulator, at 908,213 to 909,208
_0792
PP_0793: PP_0793 - Phosphotransferase system, fructose-specific EI/HPr/EIIA components, at 909,493 to 912,345
_0793
PP_0794: PP_0794 - 1-phosphofructokinase monomer, at 912,346 to 913,293
_0794
PP_0795: PP_0795 - fructose PTS permease - IIBC component, at 913,290 to 915,032
_0795
Position (kb)
909
910
911
912
913 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 908.566 kb on - strand, within PP_0792 at 908.586 kb on + strand, within PP_0792 at 908.586 kb on + strand, within PP_0792 at 908.587 kb on - strand, within PP_0792 at 908.809 kb on - strand, within PP_0792 at 908.934 kb on + strand, within PP_0792 at 908.982 kb on + strand, within PP_0792 at 908.983 kb on - strand, within PP_0792 at 908.983 kb on - strand, within PP_0792 at 909.029 kb on - strand, within PP_0792 at 909.109 kb on + strand at 909.153 kb on + strand at 909.153 kb on + strand at 909.153 kb on + strand at 909.224 kb on - strand at 909.224 kb on - strand at 909.224 kb on - strand at 909.256 kb on + strand at 909.256 kb on + strand at 909.345 kb on + strand at 909.435 kb on - strand at 909.435 kb on - strand at 909.435 kb on - strand at 909.453 kb on + strand at 909.454 kb on - strand at 909.454 kb on - strand at 909.454 kb on - strand at 909.454 kb on - strand at 909.607 kb on + strand at 909.607 kb on + strand at 909.681 kb on + strand at 909.681 kb on + strand at 909.682 kb on - strand at 909.706 kb on + strand at 909.719 kb on + strand at 909.747 kb on - strand at 909.772 kb on + strand at 909.773 kb on - strand at 909.786 kb on + strand, within PP_0793 at 909.786 kb on + strand, within PP_0793 at 909.786 kb on + strand, within PP_0793 at 909.787 kb on - strand, within PP_0793 at 910.661 kb on + strand, within PP_0793 at 910.662 kb on - strand, within PP_0793 at 910.662 kb on - strand, within PP_0793 at 911.122 kb on + strand, within PP_0793 at 911.122 kb on + strand, within PP_0793 at 911.122 kb on + strand at 911.122 kb on + strand, within PP_0793 at 911.122 kb on + strand, within PP_0793 at 911.122 kb on + strand, within PP_0793 at 911.123 kb on - strand, within PP_0793 at 911.123 kb on - strand, within PP_0793 at 911.123 kb on - strand, within PP_0793 at 911.123 kb on - strand, within PP_0793 at 911.123 kb on - strand, within PP_0793 at 911.252 kb on + strand, within PP_0793 at 911.252 kb on + strand, within PP_0793 at 911.282 kb on + strand, within PP_0793 at 911.282 kb on + strand, within PP_0793 at 911.282 kb on + strand, within PP_0793 at 911.301 kb on + strand, within PP_0793 at 911.302 kb on - strand, within PP_0793 at 911.487 kb on + strand, within PP_0793 at 911.686 kb on + strand, within PP_0793 at 911.687 kb on - strand, within PP_0793 at 911.687 kb on - strand, within PP_0793 at 911.817 kb on + strand, within PP_0793 at 911.817 kb on + strand, within PP_0793 at 911.922 kb on + strand, within PP_0793 at 912.013 kb on + strand, within PP_0793 at 912.014 kb on - strand, within PP_0793 at 912.081 kb on - strand at 912.387 kb on + strand at 912.510 kb on - strand, within PP_0794 at 912.621 kb on + strand, within PP_0794 at 912.622 kb on - strand, within PP_0794 at 912.622 kb on - strand, within PP_0794 at 912.833 kb on + strand, within PP_0794 at 912.833 kb on + strand, within PP_0794 at 912.834 kb on - strand, within PP_0794 at 912.904 kb on - strand, within PP_0794 at 913.304 kb on - strand at 913.304 kb on - strand at 913.339 kb on + strand at 913.340 kb on - strand at 913.340 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 908,566 - PP_0792 0.35 -0.9 908,586 + PP_0792 0.37 -0.8 908,586 + PP_0792 0.37 -0.0 908,587 - PP_0792 0.38 -0.7 908,809 - PP_0792 0.60 -0.4 908,934 + PP_0792 0.72 +0.1 908,982 + PP_0792 0.77 -0.1 908,983 - PP_0792 0.77 +0.6 908,983 - PP_0792 0.77 +2.3 909,029 - PP_0792 0.82 -0.1 909,109 + +0.0 909,153 + +0.3 909,153 + -0.9 909,153 + +0.4 909,224 - +1.3 909,224 - +0.1 909,224 - -0.3 909,256 + +0.7 909,256 + -0.1 909,345 + -0.7 909,435 - +1.2 909,435 - +1.1 909,435 - +0.5 909,453 + +0.9 909,454 - +2.9 909,454 - -0.5 909,454 - -0.6 909,454 - -0.9 909,607 + +1.1 909,607 + +0.6 909,681 + +1.3 909,681 + -2.8 909,682 - +0.7 909,706 + +2.1 909,719 + +1.7 909,747 - -0.3 909,772 + +1.3 909,773 - -0.8 909,786 + PP_0793 0.10 -1.1 909,786 + PP_0793 0.10 -1.9 909,786 + PP_0793 0.10 +1.8 909,787 - PP_0793 0.10 -1.8 910,661 + PP_0793 0.41 +0.1 910,662 - PP_0793 0.41 -1.4 910,662 - PP_0793 0.41 -0.9 911,122 + PP_0793 0.57 +0.3 911,122 + PP_0793 0.57 -1.0 911,122 + +3.7 911,122 + PP_0793 0.57 -1.9 911,122 + PP_0793 0.57 -0.5 911,122 + PP_0793 0.57 -1.0 911,123 - PP_0793 0.57 -0.9 911,123 - PP_0793 0.57 -1.4 911,123 - PP_0793 0.57 +0.4 911,123 - PP_0793 0.57 -0.3 911,123 - PP_0793 0.57 +0.1 911,252 + PP_0793 0.62 -0.2 911,252 + PP_0793 0.62 -0.4 911,282 + PP_0793 0.63 +0.8 911,282 + PP_0793 0.63 -0.7 911,282 + PP_0793 0.63 -0.1 911,301 + PP_0793 0.63 +0.2 911,302 - PP_0793 0.63 -0.9 911,487 + PP_0793 0.70 -1.4 911,686 + PP_0793 0.77 -1.6 911,687 - PP_0793 0.77 -0.1 911,687 - PP_0793 0.77 -0.1 911,817 + PP_0793 0.81 +2.7 911,817 + PP_0793 0.81 -1.1 911,922 + PP_0793 0.85 +2.7 912,013 + PP_0793 0.88 -0.9 912,014 - PP_0793 0.88 -1.2 912,081 - -0.6 912,387 + +0.9 912,510 - PP_0794 0.17 +1.9 912,621 + PP_0794 0.29 +2.7 912,622 - PP_0794 0.29 -0.6 912,622 - PP_0794 0.29 -0.5 912,833 + PP_0794 0.51 +2.7 912,833 + PP_0794 0.51 +2.1 912,834 - PP_0794 0.51 +2.1 912,904 - PP_0794 0.59 -0.2 913,304 - +0.8 913,304 - -0.9 913,339 + -1.9 913,340 - +0.1 913,340 - -0.3
Or see this region's nucleotide sequence