Strain Fitness in Pseudomonas putida KT2440 around PP_0244

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
298,008	+				+3.7
298,009	-				+0.3
298,049	+				-0.8
298,057	+				+0.9
298,057	+				-0.5
298,066	-				-0.7
298,066	-				+0.5
298,066	-				-0.1
298,143	+				+0.7
298,144	-				+1.2
298,158	+				-1.9
298,158	+				-1.3
298,158	+				-1.8
298,159	-				-0.2
298,159	-				-0.8
298,211	+		PP_0244	0.14	-0.4
298,211	+				+3.7
298,211	+		PP_0244	0.14	+0.4
298,211	+		PP_0244	0.14	-0.8
298,212	-		PP_0244	0.14	-1.0
298,299	+		PP_0244	0.37	-1.4
298,299	+		PP_0244	0.37	+0.0
298,299	+		PP_0244	0.37	+0.5
298,299	+		PP_0244	0.37	-2.6
298,300	-		PP_0244	0.37	-2.1
298,521	+				-0.3
298,522	-				+0.2
298,532	+				+0.8
298,532	+				+0.2
298,533	-				+0.1
298,541	+				-0.2
298,541	+				-1.1
298,541	+				-1.8
298,542	-				-0.1
298,833	+		PP_0245	0.11	-0.4
298,834	-		PP_0245	0.11	-1.4
298,834	-				+1.1
298,888	-		PP_0245	0.13	+2.7
299,022	-		PP_0245	0.19	+0.5
299,462	+		PP_0245	0.36	+0.4
299,462	+		PP_0245	0.36	+0.4
299,463	-		PP_0245	0.36	+0.4