Strain Fitness in Pseudomonas putida KT2440 around PP_0118

Experiment: 48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	48hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
122,973	-				+2.7
122,997	+				-0.1
122,998	-				-0.9
122,998	-				-0.2
123,030	+				-0.8
123,030	+				+0.5
123,031	-				+0.6
123,118	+				-0.7
123,118	+				+0.4
123,119	-				+1.7
123,119	-				+0.7
123,291	+		PP_0117	0.26	-0.4
123,292	-		PP_0117	0.26	-1.8
123,331	+		PP_0117	0.31	+0.5
123,331	+		PP_0117	0.31	+1.1
123,527	+		PP_0117	0.56	-0.0
123,527	+		PP_0117	0.56	-1.2
123,527	+		PP_0117	0.56	-0.1
123,527	+		PP_0117	0.56	+0.3
123,527	+		PP_0117	0.56	-0.2
123,528	-		PP_0117	0.57	-0.5
123,528	-		PP_0117	0.57	+0.4
123,788	-		PP_0117	0.90	+2.2
123,788	-		PP_0117	0.90	+1.0
123,951	+		PP_0118	0.12	+0.1
123,951	+		PP_0118	0.12	-1.0
123,951	+		PP_0118	0.12	-1.7
123,951	+		PP_0118	0.12	-1.5
123,952	-		PP_0118	0.12	+0.4
123,952	-		PP_0118	0.12	+0.1
123,952	-		PP_0118	0.12	+0.7
123,952	-		PP_0118	0.12	+1.0
123,952	-		PP_0118	0.12	-1.1
124,152	+		PP_0118	0.38	-1.4
124,152	+		PP_0118	0.38	-0.1
124,153	-		PP_0118	0.38	-0.9
124,153	-		PP_0118	0.38	-0.8
124,153	-		PP_0118	0.38	+0.0
124,187	+		PP_0118	0.42	-0.2
124,187	+		PP_0118	0.42	-0.1
124,188	-		PP_0118	0.42	+2.7
124,271	-		PP_0118	0.53	-0.5
124,271	-		PP_0118	0.53	-1.7
124,343	+		PP_0118	0.62	+0.7
124,344	-		PP_0118	0.63	+0.8
124,419	+		PP_0118	0.72	+1.1
124,419	+		PP_0118	0.72	-0.7
124,419	+		PP_0118	0.72	-0.6
124,420	-		PP_0118	0.72	-1.3
124,448	+		PP_0118	0.76	-1.3
124,482	-		PP_0118	0.80	-1.2
124,482	-		PP_0118	0.80	-0.3
124,650	+				-0.6
124,651	-				-1.2
124,853	-		PP_0119	0.46	+0.6
124,889	+		PP_0119	0.53	-1.8
124,889	+		PP_0119	0.53	-0.4
125,065	+		PP_0119	0.90	+0.1
125,065	+		PP_0119	0.90	-2.7
125,065	+		PP_0119	0.90	-1.3
125,066	-		PP_0119	0.90	-0.2
125,066	-		PP_0119	0.90	-0.3
125,144	+				+0.2
125,145	-				-0.5
125,145	-				-0.1
125,145	-				-1.0
125,145	-				+0.1
125,145	-				+0.4
125,147	+				-1.0
125,147	+				-0.1
125,147	+				+0.7
125,147	+				-2.2
125,148	-				+0.8
125,153	-				-0.1
125,174	+				-0.7
125,256	+				+0.6
125,256	+				-0.3
125,347	+		PP_0120	0.18	-0.3
125,398	+		PP_0120	0.24	+0.6
125,452	-		PP_0120	0.30	-2.9
125,452	-		PP_0120	0.30	-1.2
125,452	-		PP_0120	0.30	-0.7
125,572	-		PP_0120	0.43	+0.0
125,572	-		PP_0120	0.43	-1.6