Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu5045 and Atu5046 are separated by 356 nucleotides Atu5046 and Atu5047 are separated by 0 nucleotides Atu5047 and Atu5048 are separated by 84 nucleotides Atu5048 and Atu8099 are separated by 11 nucleotides
Atu5045: Atu5045 - hypothetical protein, at 45,460 to 46,200
Atu5045
Atu5046: Atu5046 - hypothetical protein, at 46,557 to 46,904
Atu5046
Atu5047: Atu5047 - hypothetical protein, at 46,905 to 47,141
Atu5047
Atu5048: Atu5048 - exodeoxyribonuclease III, at 47,226 to 48,002
Atu5048
Atu8099: Atu8099 - hypothetical protein, at 48,014 to 48,190
Atu8099
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-1
0
1
2
3 at 46.308 kb on + strand at 46.323 kb on - strand at 46.425 kb on - strand at 46.444 kb on + strand at 46.455 kb on - strand at 46.455 kb on - strand at 46.520 kb on + strand at 46.529 kb on + strand at 46.585 kb on + strand at 46.633 kb on + strand, within Atu5046 at 46.729 kb on - strand, within Atu5046 at 46.764 kb on + strand, within Atu5046 at 46.792 kb on + strand, within Atu5046 at 46.800 kb on - strand, within Atu5046 at 46.857 kb on - strand, within Atu5046 at 46.945 kb on + strand, within Atu5047 at 46.953 kb on - strand, within Atu5047 at 46.965 kb on - strand, within Atu5047 at 46.989 kb on - strand, within Atu5047 at 46.993 kb on - strand, within Atu5047 at 47.027 kb on + strand, within Atu5047 at 47.059 kb on + strand, within Atu5047 at 47.125 kb on - strand at 47.157 kb on - strand at 47.157 kb on - strand at 47.170 kb on - strand at 47.188 kb on + strand at 47.240 kb on + strand at 47.240 kb on + strand at 47.245 kb on + strand at 47.253 kb on - strand at 47.253 kb on - strand at 47.275 kb on + strand at 47.283 kb on - strand at 47.307 kb on + strand, within Atu5048 at 47.307 kb on + strand, within Atu5048 at 47.315 kb on - strand, within Atu5048 at 47.315 kb on - strand, within Atu5048 at 47.327 kb on + strand, within Atu5048 at 47.327 kb on + strand, within Atu5048 at 47.327 kb on - strand, within Atu5048 at 47.335 kb on - strand, within Atu5048 at 47.417 kb on + strand, within Atu5048 at 47.446 kb on + strand, within Atu5048 at 47.455 kb on + strand, within Atu5048 at 47.459 kb on - strand, within Atu5048 at 47.469 kb on + strand, within Atu5048 at 47.477 kb on - strand, within Atu5048 at 47.477 kb on - strand, within Atu5048 at 47.491 kb on + strand, within Atu5048 at 47.491 kb on + strand, within Atu5048 at 47.494 kb on - strand, within Atu5048 at 47.598 kb on - strand, within Atu5048 at 47.600 kb on + strand, within Atu5048 at 47.608 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.622 kb on - strand, within Atu5048 at 47.652 kb on + strand, within Atu5048 at 47.786 kb on + strand, within Atu5048 at 47.925 kb on - strand at 47.954 kb on - strand at 47.973 kb on - strand at 48.017 kb on + strand at 48.038 kb on + strand, within Atu8099 at 48.103 kb on + strand, within Atu8099 at 48.126 kb on + strand, within Atu8099
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 46,308 + +3.0 46,323 - -1.0 46,425 - -1.0 46,444 + -0.3 46,455 - +0.3 46,455 - +0.9 46,520 + +0.1 46,529 + -0.5 46,585 + -0.3 46,633 + Atu5046 0.22 +0.3 46,729 - Atu5046 0.49 +1.6 46,764 + Atu5046 0.59 -0.2 46,792 + Atu5046 0.68 +0.1 46,800 - Atu5046 0.70 +0.4 46,857 - Atu5046 0.86 -1.6 46,945 + Atu5047 0.17 +1.6 46,953 - Atu5047 0.20 +0.6 46,965 - Atu5047 0.25 +0.6 46,989 - Atu5047 0.35 +0.3 46,993 - Atu5047 0.37 +0.7 47,027 + Atu5047 0.51 +0.5 47,059 + Atu5047 0.65 +0.2 47,125 - -0.2 47,157 - +0.4 47,157 - +0.0 47,170 - +0.1 47,188 + +0.2 47,240 + +1.5 47,240 + -0.1 47,245 + +1.9 47,253 - -0.1 47,253 - +1.0 47,275 + +0.0 47,283 - +1.2 47,307 + Atu5048 0.10 -0.2 47,307 + Atu5048 0.10 -0.0 47,315 - Atu5048 0.11 +0.3 47,315 - Atu5048 0.11 -0.0 47,327 + Atu5048 0.13 +0.8 47,327 + Atu5048 0.13 -0.3 47,327 - Atu5048 0.13 +0.2 47,335 - Atu5048 0.14 +0.5 47,417 + Atu5048 0.25 +0.2 47,446 + Atu5048 0.28 -0.5 47,455 + Atu5048 0.29 -0.6 47,459 - Atu5048 0.30 +0.7 47,469 + Atu5048 0.31 -0.7 47,477 - Atu5048 0.32 -0.6 47,477 - Atu5048 0.32 +0.7 47,491 + Atu5048 0.34 -0.1 47,491 + Atu5048 0.34 -0.9 47,494 - Atu5048 0.34 +0.0 47,598 - Atu5048 0.48 +0.4 47,600 + Atu5048 0.48 -0.7 47,608 - Atu5048 0.49 +0.2 47,622 - Atu5048 0.51 +1.1 47,622 - Atu5048 0.51 +1.2 47,622 - Atu5048 0.51 +1.0 47,622 - Atu5048 0.51 +1.8 47,652 + Atu5048 0.55 -1.5 47,786 + Atu5048 0.72 -0.8 47,925 - +0.1 47,954 - -0.2 47,973 - -0.8 48,017 + -0.4 48,038 + Atu8099 0.14 -0.6 48,103 + Atu8099 0.50 +0.9 48,126 + Atu8099 0.63 +0.2
Or see this region's nucleotide sequence