Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1002 and Atu1003 are separated by 65 nucleotides Atu1003 and Atu1004 are separated by 3 nucleotides Atu1004 and Atu1005 overlap by 4 nucleotides Atu1005 and Atu1006 are separated by 66 nucleotides
Atu1002: Atu1002 - Aminotransferase class-III, at 999,240 to 1,000,670
Atu1002
Atu1003: Atu1003 - hypothetical protein, at 1,000,736 to 1,001,206
Atu1003
Atu1004: Atu1004 - hypothetical protein, at 1,001,210 to 1,001,599
Atu1004
Atu1005: Atu1005 - hypothetical protein, at 1,001,596 to 1,001,826
Atu1005
Atu1006: Atu1006 - AMP nucleosidase, at 1,001,893 to 1,003,398
Atu1006
Position (kb)
1000
1001
1002 Strain fitness (log2 ratio)
-1
0
1 at 999.854 kb on + strand, within Atu1002 at 999.862 kb on - strand, within Atu1002 at 1000.001 kb on - strand, within Atu1002 at 1000.058 kb on - strand, within Atu1002 at 1000.336 kb on - strand, within Atu1002 at 1000.385 kb on - strand, within Atu1002 at 1000.457 kb on + strand, within Atu1002 at 1000.547 kb on + strand at 1000.581 kb on - strand at 1000.594 kb on + strand at 1000.878 kb on - strand, within Atu1003 at 1000.934 kb on + strand, within Atu1003 at 1000.944 kb on + strand, within Atu1003 at 1001.041 kb on - strand, within Atu1003 at 1001.048 kb on + strand, within Atu1003 at 1001.056 kb on - strand, within Atu1003 at 1001.107 kb on - strand, within Atu1003 at 1001.200 kb on + strand at 1001.208 kb on - strand at 1001.319 kb on + strand, within Atu1004 at 1001.343 kb on - strand, within Atu1004 at 1001.358 kb on - strand, within Atu1004 at 1001.358 kb on - strand, within Atu1004 at 1001.530 kb on + strand, within Atu1004 at 1001.583 kb on - strand at 1001.616 kb on + strand at 1001.660 kb on - strand, within Atu1005 at 1001.704 kb on - strand, within Atu1005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 999,854 + Atu1002 0.43 +0.1 999,862 - Atu1002 0.43 -0.1 1,000,001 - Atu1002 0.53 +0.8 1,000,058 - Atu1002 0.57 +0.1 1,000,336 - Atu1002 0.77 -0.2 1,000,385 - Atu1002 0.80 -0.4 1,000,457 + Atu1002 0.85 +0.2 1,000,547 + -1.0 1,000,581 - +0.5 1,000,594 + -0.2 1,000,878 - Atu1003 0.30 +0.4 1,000,934 + Atu1003 0.42 +0.3 1,000,944 + Atu1003 0.44 -1.0 1,001,041 - Atu1003 0.65 +0.1 1,001,048 + Atu1003 0.66 -0.7 1,001,056 - Atu1003 0.68 -0.4 1,001,107 - Atu1003 0.79 -0.5 1,001,200 + -0.6 1,001,208 - -0.1 1,001,319 + Atu1004 0.28 -0.6 1,001,343 - Atu1004 0.34 -0.4 1,001,358 - Atu1004 0.38 -0.0 1,001,358 - Atu1004 0.38 -1.0 1,001,530 + Atu1004 0.82 -0.1 1,001,583 - +0.7 1,001,616 + -0.1 1,001,660 - Atu1005 0.28 +0.1 1,001,704 - Atu1005 0.47 -0.2
Or see this region's nucleotide sequence