Experiment: R2A with Vogels_fungal_media 0.8X
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1000 and Atu1001 are separated by 394 nucleotides Atu1001 and Atu1002 are separated by 89 nucleotides
Atu1000: Atu1000 - MFS permease, at 996,762 to 997,847
Atu1000
Atu1001: Atu1001 - hypothetical protein, at 998,242 to 999,150
Atu1001
Atu1002: Atu1002 - Aminotransferase class-III, at 999,240 to 1,000,670
Atu1002
Position (kb)
998
999
1000 Strain fitness (log2 ratio)
-2
-1
0
1 at 997.242 kb on - strand, within Atu1000 at 997.252 kb on + strand, within Atu1000 at 997.314 kb on - strand, within Atu1000 at 997.357 kb on - strand, within Atu1000 at 997.402 kb on + strand, within Atu1000 at 997.402 kb on + strand, within Atu1000 at 997.410 kb on - strand, within Atu1000 at 997.449 kb on + strand, within Atu1000 at 997.449 kb on + strand, within Atu1000 at 997.452 kb on - strand, within Atu1000 at 997.457 kb on - strand, within Atu1000 at 997.493 kb on + strand, within Atu1000 at 997.516 kb on - strand, within Atu1000 at 997.540 kb on + strand, within Atu1000 at 997.542 kb on + strand, within Atu1000 at 997.563 kb on - strand, within Atu1000 at 997.628 kb on + strand, within Atu1000 at 997.636 kb on - strand, within Atu1000 at 997.897 kb on + strand at 997.930 kb on - strand at 997.930 kb on - strand at 997.947 kb on + strand at 997.965 kb on + strand at 997.967 kb on + strand at 998.063 kb on + strand at 998.326 kb on + strand at 998.334 kb on - strand, within Atu1001 at 998.357 kb on + strand, within Atu1001 at 998.358 kb on - strand, within Atu1001 at 998.384 kb on - strand, within Atu1001 at 998.407 kb on - strand, within Atu1001 at 998.477 kb on + strand, within Atu1001 at 998.481 kb on - strand, within Atu1001 at 998.509 kb on - strand, within Atu1001 at 998.540 kb on + strand, within Atu1001 at 998.579 kb on - strand, within Atu1001 at 998.740 kb on + strand, within Atu1001 at 998.924 kb on + strand, within Atu1001 at 999.150 kb on + strand at 999.177 kb on + strand at 999.214 kb on - strand at 999.283 kb on - strand at 999.525 kb on - strand, within Atu1002 at 999.571 kb on + strand, within Atu1002 at 999.854 kb on + strand, within Atu1002 at 999.862 kb on - strand, within Atu1002 at 1000.001 kb on - strand, within Atu1002 at 1000.058 kb on - strand, within Atu1002
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vogels_fungal_media 0.8X remove 997,242 - Atu1000 0.44 +0.8 997,252 + Atu1000 0.45 -0.0 997,314 - Atu1000 0.51 -0.4 997,357 - Atu1000 0.55 -0.1 997,402 + Atu1000 0.59 +0.1 997,402 + Atu1000 0.59 -2.0 997,410 - Atu1000 0.60 +0.2 997,449 + Atu1000 0.63 -1.1 997,449 + Atu1000 0.63 -0.4 997,452 - Atu1000 0.64 -0.0 997,457 - Atu1000 0.64 +0.2 997,493 + Atu1000 0.67 -0.2 997,516 - Atu1000 0.69 +0.2 997,540 + Atu1000 0.72 -1.8 997,542 + Atu1000 0.72 -0.0 997,563 - Atu1000 0.74 +1.0 997,628 + Atu1000 0.80 -0.4 997,636 - Atu1000 0.80 +0.7 997,897 + -1.3 997,930 - -0.5 997,930 - -0.3 997,947 + -0.2 997,965 + -0.7 997,967 + +0.5 998,063 + +1.0 998,326 + +0.9 998,334 - Atu1001 0.10 +0.0 998,357 + Atu1001 0.13 -0.1 998,358 - Atu1001 0.13 -0.3 998,384 - Atu1001 0.16 -0.6 998,407 - Atu1001 0.18 -1.1 998,477 + Atu1001 0.26 -0.2 998,481 - Atu1001 0.26 -0.4 998,509 - Atu1001 0.29 +0.5 998,540 + Atu1001 0.33 +0.3 998,579 - Atu1001 0.37 -0.0 998,740 + Atu1001 0.55 -0.4 998,924 + Atu1001 0.75 +1.3 999,150 + -0.4 999,177 + +0.1 999,214 - +0.1 999,283 - +0.0 999,525 - Atu1002 0.20 -0.3 999,571 + Atu1002 0.23 -0.1 999,854 + Atu1002 0.43 +0.1 999,862 - Atu1002 0.43 -0.1 1,000,001 - Atu1002 0.53 +0.8 1,000,058 - Atu1002 0.57 +0.1
Or see this region's nucleotide sequence