Strain Fitness in Escherichia coli BW25113 around b4258

Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjgN and valS are separated by 55 nucleotidesvalS and holC overlap by 1 nucleotidesholC and pepA are separated by 159 nucleotides b4257: yjgN - conserved inner membrane protein (NCBI), at 4,477,753 to 4,478,949 yjgN b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860 valS b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303 holC b4260: pepA - leucyl aminopeptidase (NCBI), at 4,482,463 to 4,483,974 pepA Position (kb) 4479 4480 4481 4482Strain fitness (log2 ratio) -2 -1 0 1 2at 4478.268 kb on + strand, within yjgNat 4478.268 kb on + strand, within yjgNat 4478.343 kb on + strand, within yjgNat 4478.530 kb on + strand, within yjgNat 4478.668 kb on + strand, within yjgNat 4478.675 kb on + strand, within yjgNat 4478.675 kb on + strand, within yjgNat 4478.678 kb on - strand, within yjgNat 4478.702 kb on - strand, within yjgNat 4478.702 kb on - strand, within yjgNat 4478.726 kb on + strand, within yjgNat 4478.727 kb on + strand, within yjgNat 4478.727 kb on + strand, within yjgNat 4478.727 kb on + strand, within yjgNat 4478.741 kb on + strand, within yjgNat 4478.744 kb on + strand, within yjgNat 4478.747 kb on + strand, within yjgNat 4478.750 kb on + strand, within yjgNat 4478.770 kb on + strand, within yjgNat 4478.770 kb on - strand, within yjgNat 4478.780 kb on + strand, within yjgNat 4478.784 kb on + strand, within yjgNat 4478.789 kb on + strand, within yjgNat 4478.858 kb on + strandat 4478.858 kb on + strandat 4478.898 kb on - strandat 4478.926 kb on + strandat 4478.926 kb on + strandat 4478.930 kb on + strandat 4482.341 kb on + strandat 4482.349 kb on - strandat 4482.354 kb on - strandat 4482.369 kb on - strandat 4482.369 kb on - strandat 4482.379 kb on - strandat 4482.394 kb on + strandat 4482.394 kb on - strandat 4482.402 kb on - strandat 4482.402 kb on - strandat 4482.404 kb on + strandat 4482.445 kb on + strandat 4482.452 kb on + strandat 4482.458 kb on + strandat 4482.460 kb on + strandat 4482.494 kb on + strandat 4482.582 kb on - strandat 4482.650 kb on + strand, within pepAat 4482.690 kb on - strand, within pepAat 4482.698 kb on - strand, within pepAat 4482.706 kb on + strandat 4482.706 kb on + strandat 4482.726 kb on - strand, within pepAat 4482.741 kb on + strand, within pepAat 4482.749 kb on - strand, within pepAat 4482.749 kb on - strand, within pepAat 4482.749 kb on - strand, within pepAat 4482.787 kb on + strand, within pepAat 4482.787 kb on + strand, within pepAat 4482.800 kb on - strand, within pepAat 4482.805 kb on - strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.822 kb on + strand, within pepAat 4482.822 kb on + strand, within pepA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM
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4,478,268 + yjgN b4257 0.43 +1.1
4,478,268 + yjgN b4257 0.43 +1.1
4,478,343 + yjgN b4257 0.49 +1.1
4,478,530 + yjgN b4257 0.65 +1.1
4,478,668 + yjgN b4257 0.76 -1.2
4,478,675 + yjgN b4257 0.77 +2.1
4,478,675 + yjgN b4257 0.77 +0.1
4,478,678 - yjgN b4257 0.77 +1.1
4,478,702 - yjgN b4257 0.79 +1.1
4,478,702 - yjgN b4257 0.79 -0.5
4,478,726 + yjgN b4257 0.81 -1.9
4,478,727 + yjgN b4257 0.81 +0.1
4,478,727 + yjgN b4257 0.81 -0.0
4,478,727 + yjgN b4257 0.81 -1.5
4,478,741 + yjgN b4257 0.83 -1.9
4,478,744 + yjgN b4257 0.83 +0.3
4,478,747 + yjgN b4257 0.83 -0.6
4,478,750 + yjgN b4257 0.83 +0.1
4,478,770 + yjgN b4257 0.85 +0.4
4,478,770 - yjgN b4257 0.85 -0.5
4,478,780 + yjgN b4257 0.86 +0.2
4,478,784 + yjgN b4257 0.86 -0.9
4,478,789 + yjgN b4257 0.87 +0.2
4,478,858 + +0.7
4,478,858 + +0.9
4,478,898 - -1.7
4,478,926 + +0.9
4,478,926 + +0.2
4,478,930 + +0.8
4,482,341 + -1.5
4,482,349 - +0.2
4,482,354 - -0.5
4,482,369 - +0.2
4,482,369 - -0.1
4,482,379 - +0.5
4,482,394 + -0.9
4,482,394 - +0.5
4,482,402 - -0.1
4,482,402 - -0.1
4,482,404 + +0.4
4,482,445 + -0.2
4,482,452 + -0.5
4,482,458 + -0.5
4,482,460 + -0.7
4,482,494 + -1.5
4,482,582 - -0.8
4,482,650 + pepA b4260 0.12 -1.9
4,482,690 - pepA b4260 0.15 -0.5
4,482,698 - pepA b4260 0.16 -0.6
4,482,706 + -0.5
4,482,706 + +1.1
4,482,726 - pepA b4260 0.17 -0.5
4,482,741 + pepA b4260 0.18 +0.3
4,482,749 - pepA b4260 0.19 -1.7
4,482,749 - pepA b4260 0.19 -1.2
4,482,749 - pepA b4260 0.19 +1.1
4,482,787 + pepA b4260 0.21 +1.5
4,482,787 + pepA b4260 0.21 -2.2
4,482,800 - pepA b4260 0.22 -1.2
4,482,805 - pepA b4260 0.23 -1.0
4,482,822 + pepA b4260 0.24 -0.5
4,482,822 + pepA b4260 0.24 +1.1
4,482,822 + pepA b4260 0.24 -0.1
4,482,822 + pepA b4260 0.24 +0.1

Or see this region's nucleotide sequence