Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gidA and mioC are separated by 378 nucleotides mioC and asnC are separated by 89 nucleotides asnC and asnA are separated by 151 nucleotides
b3741: gidA - glucose-inhibited division protein A (NCBI), at 3,921,767 to 3,923,656
gidA
b3742: mioC - flavodoxin (NCBI), at 3,924,035 to 3,924,478
mioC
b3743: asnC - DNA-binding transcriptional dual regulator (NCBI), at 3,924,568 to 3,925,026
asnC
b3744: asnA - asparagine synthetase AsnA (NCBI), at 3,925,178 to 3,926,170
asnA
Position (kb)
3924
3925 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3923.097 kb on - strand, within gidA at 3923.136 kb on - strand, within gidA at 3923.142 kb on + strand, within gidA at 3923.245 kb on - strand, within gidA at 3923.251 kb on - strand, within gidA at 3923.277 kb on - strand, within gidA at 3923.326 kb on - strand, within gidA at 3923.332 kb on + strand, within gidA at 3923.374 kb on - strand, within gidA at 3923.374 kb on - strand, within gidA at 3923.374 kb on - strand, within gidA at 3923.409 kb on + strand, within gidA at 3923.470 kb on - strand at 3923.506 kb on + strand at 3923.525 kb on - strand at 3923.535 kb on - strand at 3923.579 kb on - strand at 3923.597 kb on - strand at 3923.608 kb on + strand at 3923.721 kb on - strand at 3924.034 kb on + strand at 3924.034 kb on + strand at 3924.159 kb on + strand, within mioC at 3924.205 kb on + strand, within mioC at 3924.206 kb on + strand, within mioC at 3924.206 kb on + strand, within mioC at 3924.307 kb on + strand, within mioC at 3924.323 kb on - strand, within mioC at 3924.330 kb on - strand, within mioC at 3924.447 kb on - strand at 3924.458 kb on + strand at 3924.458 kb on + strand at 3924.465 kb on + strand at 3924.471 kb on - strand at 3924.507 kb on + strand at 3924.507 kb on + strand at 3924.507 kb on + strand at 3924.515 kb on + strand at 3924.515 kb on - strand at 3924.515 kb on - strand at 3924.515 kb on - strand at 3924.525 kb on - strand at 3924.530 kb on + strand at 3924.664 kb on + strand, within asnC at 3924.668 kb on - strand, within asnC at 3924.668 kb on - strand, within asnC at 3924.672 kb on - strand, within asnC at 3924.696 kb on + strand, within asnC at 3924.696 kb on + strand, within asnC at 3924.708 kb on - strand, within asnC at 3924.720 kb on - strand, within asnC at 3924.720 kb on - strand, within asnC at 3924.720 kb on - strand, within asnC at 3924.722 kb on + strand, within asnC at 3924.730 kb on - strand, within asnC at 3924.730 kb on - strand, within asnC at 3924.742 kb on - strand, within asnC at 3924.742 kb on - strand, within asnC at 3924.793 kb on - strand, within asnC at 3924.796 kb on + strand, within asnC at 3924.823 kb on - strand, within asnC at 3924.840 kb on + strand, within asnC at 3924.850 kb on - strand, within asnC at 3924.852 kb on + strand, within asnC at 3924.860 kb on - strand, within asnC at 3924.904 kb on + strand, within asnC at 3924.911 kb on + strand, within asnC at 3924.911 kb on + strand, within asnC at 3924.923 kb on - strand, within asnC at 3924.965 kb on + strand, within asnC at 3924.990 kb on - strand at 3925.206 kb on + strand at 3925.206 kb on + strand at 3925.210 kb on + strand at 3925.254 kb on + strand at 3925.301 kb on - strand, within asnA at 3925.301 kb on - strand, within asnA at 3925.301 kb on - strand, within asnA at 3925.305 kb on - strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.312 kb on + strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.320 kb on - strand, within asnA at 3925.348 kb on - strand, within asnA at 3925.358 kb on + strand, within asnA at 3925.388 kb on - strand, within asnA at 3925.411 kb on + strand, within asnA at 3925.417 kb on - strand, within asnA at 3925.443 kb on + strand, within asnA at 3925.443 kb on + strand, within asnA at 3925.458 kb on - strand, within asnA at 3925.462 kb on - strand, within asnA at 3925.470 kb on - strand, within asnA at 3925.470 kb on - strand, within asnA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM remove 3,923,097 - gidA b3741 0.70 +2.9 3,923,136 - gidA b3741 0.72 +2.4 3,923,142 + gidA b3741 0.73 +1.4 3,923,245 - gidA b3741 0.78 -0.6 3,923,251 - gidA b3741 0.79 +1.4 3,923,277 - gidA b3741 0.80 -1.0 3,923,326 - gidA b3741 0.82 -0.6 3,923,332 + gidA b3741 0.83 +1.4 3,923,374 - gidA b3741 0.85 +1.4 3,923,374 - gidA b3741 0.85 +1.4 3,923,374 - gidA b3741 0.85 +1.4 3,923,409 + gidA b3741 0.87 +1.4 3,923,470 - +1.4 3,923,506 + -0.2 3,923,525 - +1.4 3,923,535 - +0.4 3,923,579 - +0.4 3,923,597 - +1.4 3,923,608 + +0.4 3,923,721 - +0.5 3,924,034 + +0.7 3,924,034 + +1.0 3,924,159 + mioC b3742 0.28 -1.7 3,924,205 + mioC b3742 0.38 -1.0 3,924,206 + mioC b3742 0.39 -1.8 3,924,206 + mioC b3742 0.39 -0.8 3,924,307 + mioC b3742 0.61 -2.1 3,924,323 - mioC b3742 0.65 -1.2 3,924,330 - mioC b3742 0.66 -0.0 3,924,447 - -1.1 3,924,458 + -0.4 3,924,458 + -0.1 3,924,465 + -0.0 3,924,471 - -0.4 3,924,507 + +0.3 3,924,507 + +0.2 3,924,507 + -0.2 3,924,515 + +0.6 3,924,515 - -0.4 3,924,515 - -0.7 3,924,515 - -1.0 3,924,525 - +0.9 3,924,530 + +0.5 3,924,664 + asnC b3743 0.21 +1.8 3,924,668 - asnC b3743 0.22 -0.2 3,924,668 - asnC b3743 0.22 +0.6 3,924,672 - asnC b3743 0.23 -0.5 3,924,696 + asnC b3743 0.28 +0.5 3,924,696 + asnC b3743 0.28 -0.4 3,924,708 - asnC b3743 0.31 +0.4 3,924,720 - asnC b3743 0.33 +0.1 3,924,720 - asnC b3743 0.33 -0.5 3,924,720 - asnC b3743 0.33 +0.8 3,924,722 + asnC b3743 0.34 +0.2 3,924,730 - asnC b3743 0.35 -1.5 3,924,730 - asnC b3743 0.35 +1.0 3,924,742 - asnC b3743 0.38 +1.1 3,924,742 - asnC b3743 0.38 -0.1 3,924,793 - asnC b3743 0.49 -0.2 3,924,796 + asnC b3743 0.50 +0.1 3,924,823 - asnC b3743 0.56 +0.3 3,924,840 + asnC b3743 0.59 +0.9 3,924,850 - asnC b3743 0.61 -1.2 3,924,852 + asnC b3743 0.62 +1.4 3,924,860 - asnC b3743 0.64 -0.3 3,924,904 + asnC b3743 0.73 -0.3 3,924,911 + asnC b3743 0.75 +0.4 3,924,911 + asnC b3743 0.75 +2.9 3,924,923 - asnC b3743 0.77 +0.9 3,924,965 + asnC b3743 0.86 -0.4 3,924,990 - +0.0 3,925,206 + -0.4 3,925,206 + -0.6 3,925,210 + -0.4 3,925,254 + +1.1 3,925,301 - asnA b3744 0.12 +0.8 3,925,301 - asnA b3744 0.12 +0.5 3,925,301 - asnA b3744 0.12 +0.4 3,925,305 - asnA b3744 0.13 -0.8 3,925,312 + asnA b3744 0.13 -0.1 3,925,312 + asnA b3744 0.13 +1.6 3,925,312 + asnA b3744 0.13 -0.4 3,925,320 - asnA b3744 0.14 +0.2 3,925,320 - asnA b3744 0.14 -0.1 3,925,320 - asnA b3744 0.14 -0.6 3,925,348 - asnA b3744 0.17 -0.4 3,925,358 + asnA b3744 0.18 +1.4 3,925,388 - asnA b3744 0.21 +0.4 3,925,411 + asnA b3744 0.23 -1.2 3,925,417 - asnA b3744 0.24 +0.1 3,925,443 + asnA b3744 0.27 -0.0 3,925,443 + asnA b3744 0.27 +0.2 3,925,458 - asnA b3744 0.28 +0.5 3,925,462 - asnA b3744 0.29 +0.8 3,925,470 - asnA b3744 0.29 -1.4 3,925,470 - asnA b3744 0.29 +0.4
Or see this region's nucleotide sequence