Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atpE and atpB are separated by 46 nucleotides atpB and atpI are separated by 8 nucleotides atpI and gidB are separated by 616 nucleotides
b3737: atpE - F0F1 ATP synthase subunit C (NCBI), at 3,918,973 to 3,919,212
atpE
b3738: atpB - F0F1 ATP synthase subunit A (NCBI), at 3,919,259 to 3,920,074
atpB
b3739: atpI - membrane-bound ATP synthase, dispensable protein, affects expression of atpB (VIMSS), at 3,920,083 to 3,920,463
atpI
b3740: gidB - glucose-inhibited division protein B (NCBI), at 3,921,080 to 3,921,703
gidB
Position (kb)
3920
3921 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3919.227 kb on - strand at 3919.248 kb on - strand at 3919.265 kb on - strand at 3919.265 kb on - strand at 3920.048 kb on - strand at 3920.063 kb on - strand at 3920.063 kb on - strand at 3920.119 kb on - strand at 3920.212 kb on - strand, within atpI at 3920.212 kb on - strand, within atpI at 3920.223 kb on - strand, within atpI at 3920.270 kb on - strand, within atpI at 3920.270 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.362 kb on - strand, within atpI at 3920.365 kb on - strand, within atpI at 3920.375 kb on - strand, within atpI at 3920.408 kb on - strand, within atpI at 3920.410 kb on - strand, within atpI at 3920.412 kb on - strand, within atpI at 3920.425 kb on - strand, within atpI at 3920.462 kb on - strand at 3920.488 kb on - strand at 3920.511 kb on - strand at 3920.514 kb on - strand at 3920.533 kb on - strand at 3920.538 kb on - strand at 3920.558 kb on - strand at 3920.561 kb on - strand at 3920.594 kb on - strand at 3920.594 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.638 kb on - strand at 3920.641 kb on + strand at 3920.649 kb on - strand at 3920.651 kb on + strand at 3920.658 kb on - strand at 3920.675 kb on + strand at 3920.675 kb on + strand at 3920.696 kb on - strand at 3920.696 kb on - strand at 3920.698 kb on + strand at 3920.699 kb on + strand at 3920.699 kb on + strand at 3920.707 kb on + strand at 3920.707 kb on - strand at 3920.717 kb on + strand at 3920.722 kb on + strand at 3920.722 kb on + strand at 3920.722 kb on + strand at 3920.725 kb on - strand at 3920.726 kb on - strand at 3920.726 kb on - strand at 3920.729 kb on - strand at 3920.729 kb on - strand at 3920.751 kb on - strand at 3920.768 kb on + strand at 3920.773 kb on + strand at 3920.867 kb on + strand at 3920.877 kb on + strand at 3920.885 kb on - strand at 3920.885 kb on - strand at 3920.920 kb on - strand at 3920.920 kb on - strand at 3920.963 kb on + strand at 3920.963 kb on + strand at 3920.982 kb on - strand at 3920.985 kb on + strand at 3920.992 kb on + strand at 3920.997 kb on + strand at 3920.997 kb on + strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.014 kb on - strand at 3921.036 kb on + strand at 3921.039 kb on + strand at 3921.039 kb on + strand at 3921.047 kb on - strand at 3921.101 kb on - strand at 3921.113 kb on - strand at 3921.113 kb on - strand at 3921.122 kb on + strand at 3921.122 kb on + strand at 3921.125 kb on - strand at 3921.125 kb on - strand at 3921.125 kb on - strand at 3921.225 kb on - strand, within gidB at 3921.240 kb on + strand, within gidB at 3921.252 kb on - strand, within gidB at 3921.255 kb on + strand, within gidB at 3921.263 kb on - strand, within gidB at 3921.265 kb on - strand, within gidB at 3921.310 kb on - strand, within gidB at 3921.336 kb on - strand, within gidB at 3921.336 kb on - strand, within gidB at 3921.336 kb on - strand, within gidB at 3921.338 kb on + strand, within gidB at 3921.357 kb on - strand, within gidB at 3921.390 kb on - strand, within gidB at 3921.406 kb on + strand, within gidB at 3921.406 kb on + strand, within gidB at 3921.414 kb on + strand, within gidB at 3921.414 kb on + strand, within gidB at 3921.424 kb on - strand, within gidB at 3921.424 kb on - strand, within gidB at 3921.434 kb on - strand, within gidB at 3921.434 kb on - strand, within gidB at 3921.445 kb on + strand, within gidB at 3921.450 kb on + strand, within gidB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM remove 3,919,227 - +1.4 3,919,248 - -0.2 3,919,265 - -1.6 3,919,265 - -0.5 3,920,048 - -0.6 3,920,063 - -2.0 3,920,063 - -2.0 3,920,119 - +0.0 3,920,212 - atpI b3739 0.34 -0.7 3,920,212 - atpI b3739 0.34 -1.4 3,920,223 - atpI b3739 0.37 -1.0 3,920,270 - atpI b3739 0.49 -0.4 3,920,270 - atpI b3739 0.49 +0.4 3,920,362 - atpI b3739 0.73 -0.4 3,920,362 - atpI b3739 0.73 -3.0 3,920,362 - atpI b3739 0.73 +0.5 3,920,365 - atpI b3739 0.74 +0.0 3,920,375 - atpI b3739 0.77 -2.3 3,920,408 - atpI b3739 0.85 -1.0 3,920,410 - atpI b3739 0.86 -1.3 3,920,412 - atpI b3739 0.86 -0.1 3,920,425 - atpI b3739 0.90 -0.2 3,920,462 - +0.2 3,920,488 - -1.1 3,920,511 - +0.2 3,920,514 - -1.3 3,920,533 - -2.1 3,920,538 - -1.2 3,920,558 - +0.1 3,920,561 - +0.1 3,920,594 - -0.6 3,920,594 - -1.2 3,920,638 - -1.0 3,920,638 - +0.2 3,920,638 - -1.4 3,920,638 - -1.1 3,920,641 + +0.0 3,920,649 - -0.2 3,920,651 + +1.4 3,920,658 - -0.6 3,920,675 + +0.4 3,920,675 + -0.8 3,920,696 - -1.4 3,920,696 - -1.0 3,920,698 + +0.6 3,920,699 + -0.2 3,920,699 + +0.8 3,920,707 + -1.5 3,920,707 - +0.4 3,920,717 + +1.0 3,920,722 + -1.7 3,920,722 + -1.1 3,920,722 + +0.7 3,920,725 - +0.4 3,920,726 - +0.0 3,920,726 - -0.8 3,920,729 - -0.2 3,920,729 - +0.8 3,920,751 - -0.6 3,920,768 + -0.2 3,920,773 + -2.4 3,920,867 + -1.2 3,920,877 + -1.5 3,920,885 - +0.1 3,920,885 - +1.1 3,920,920 - -0.7 3,920,920 - +0.7 3,920,963 + +0.7 3,920,963 + -2.3 3,920,982 - +1.1 3,920,985 + -0.4 3,920,992 + +0.4 3,920,997 + +0.0 3,920,997 + -0.3 3,921,014 - -0.3 3,921,014 - -0.3 3,921,014 - +0.6 3,921,014 - +0.8 3,921,036 + +1.1 3,921,039 + +1.1 3,921,039 + +1.4 3,921,047 - +0.4 3,921,101 - +0.1 3,921,113 - +0.4 3,921,113 - +0.4 3,921,122 + +0.5 3,921,122 + +1.0 3,921,125 - -0.1 3,921,125 - -1.0 3,921,125 - +0.9 3,921,225 - gidB b3740 0.23 -1.5 3,921,240 + gidB b3740 0.26 +1.0 3,921,252 - gidB b3740 0.28 -1.1 3,921,255 + gidB b3740 0.28 -0.6 3,921,263 - gidB b3740 0.29 +0.6 3,921,265 - gidB b3740 0.30 +0.8 3,921,310 - gidB b3740 0.37 +0.2 3,921,336 - gidB b3740 0.41 -0.4 3,921,336 - gidB b3740 0.41 +0.4 3,921,336 - gidB b3740 0.41 +0.6 3,921,338 + gidB b3740 0.41 +0.1 3,921,357 - gidB b3740 0.44 +0.5 3,921,390 - gidB b3740 0.50 -0.3 3,921,406 + gidB b3740 0.52 -1.1 3,921,406 + gidB b3740 0.52 +0.2 3,921,414 + gidB b3740 0.54 -0.1 3,921,414 + gidB b3740 0.54 +0.3 3,921,424 - gidB b3740 0.55 -1.0 3,921,424 - gidB b3740 0.55 -0.3 3,921,434 - gidB b3740 0.57 +0.2 3,921,434 - gidB b3740 0.57 -1.4 3,921,445 + gidB b3740 0.58 -0.6 3,921,450 + gidB b3740 0.59 +0.0
Or see this region's nucleotide sequence