Strain Fitness in Escherichia coli BW25113 around b3350

Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntslyX and slyD are separated by 48 nucleotidesslyD and yheV are separated by 94 nucleotidesyheV and kefB are separated by 9 nucleotideskefB and kefG overlap by 1 nucleotideskefG and yheS are separated by 127 nucleotides b3348: slyX - hypothetical protein (NCBI), at 3,475,662 to 3,475,880 slyX b3349: slyD - FKBP-type peptidyl prolyl cis-trans isomerase (rotamase) (NCBI), at 3,475,929 to 3,476,519 slyD b4551: yheV - hypothetical protein (NCBI), at 3,476,614 to 3,476,814 yheV b3350: kefB - glutathione-regulated potassium-efflux system protein (NCBI), at 3,476,824 to 3,478,629 kefB b3351: kefG - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 3,478,629 to 3,479,183 kefG b3352: yheS - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,479,311 to 3,481,224 yheS Position (kb) 3476 3477 3478 3479Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 3475.861 kb on + strandat 3475.874 kb on + strandat 3475.874 kb on + strandat 3475.939 kb on - strandat 3475.971 kb on + strandat 3475.991 kb on - strand, within slyDat 3475.991 kb on - strand, within slyDat 3476.000 kb on - strand, within slyDat 3476.042 kb on + strandat 3476.042 kb on + strand, within slyDat 3476.061 kb on + strand, within slyDat 3476.061 kb on + strand, within slyDat 3476.063 kb on + strand, within slyDat 3476.063 kb on + strand, within slyDat 3476.089 kb on - strand, within slyDat 3476.089 kb on - strand, within slyDat 3476.114 kb on - strand, within slyDat 3476.143 kb on + strand, within slyDat 3476.206 kb on + strand, within slyDat 3476.212 kb on - strand, within slyDat 3476.242 kb on + strand, within slyDat 3476.286 kb on + strand, within slyDat 3476.333 kb on - strand, within slyDat 3476.378 kb on - strand, within slyDat 3476.423 kb on - strand, within slyDat 3476.423 kb on - strand, within slyDat 3476.465 kb on + strandat 3476.473 kb on - strandat 3476.473 kb on - strandat 3476.479 kb on + strandat 3476.479 kb on + strandat 3476.485 kb on - strandat 3476.485 kb on - strandat 3476.522 kb on - strandat 3476.525 kb on + strandat 3476.525 kb on + strandat 3476.525 kb on + strandat 3476.525 kb on + strandat 3476.544 kb on - strandat 3476.544 kb on - strandat 3476.549 kb on - strandat 3476.612 kb on - strandat 3476.649 kb on - strand, within yheVat 3476.768 kb on - strand, within yheVat 3476.810 kb on + strandat 3476.853 kb on - strandat 3476.917 kb on - strandat 3476.917 kb on - strandat 3476.933 kb on + strandat 3476.941 kb on - strandat 3476.941 kb on - strandat 3476.941 kb on - strandat 3476.972 kb on - strandat 3477.015 kb on - strand, within kefBat 3477.021 kb on - strandat 3477.047 kb on - strand, within kefBat 3477.096 kb on + strand, within kefBat 3477.096 kb on + strand, within kefBat 3477.101 kb on + strand, within kefBat 3477.101 kb on + strand, within kefBat 3477.109 kb on - strand, within kefBat 3477.109 kb on - strand, within kefBat 3477.111 kb on + strand, within kefBat 3477.111 kb on + strand, within kefBat 3477.113 kb on - strand, within kefBat 3477.113 kb on - strand, within kefBat 3477.129 kb on - strandat 3477.129 kb on - strand, within kefBat 3477.131 kb on + strand, within kefBat 3477.131 kb on - strand, within kefBat 3477.131 kb on - strand, within kefBat 3477.164 kb on - strand, within kefBat 3477.164 kb on - strand, within kefBat 3477.189 kb on + strand, within kefBat 3477.189 kb on + strand, within kefBat 3477.197 kb on - strand, within kefBat 3477.197 kb on - strand, within kefBat 3477.201 kb on + strand, within kefBat 3477.218 kb on - strand, within kefBat 3477.219 kb on - strand, within kefBat 3477.256 kb on + strand, within kefBat 3477.256 kb on + strand, within kefBat 3477.271 kb on - strand, within kefBat 3477.271 kb on - strand, within kefBat 3477.313 kb on - strand, within kefBat 3477.313 kb on - strand, within kefBat 3477.326 kb on + strand, within kefBat 3477.337 kb on - strand, within kefBat 3477.337 kb on - strand, within kefBat 3477.350 kb on - strand, within kefBat 3477.388 kb on + strand, within kefBat 3477.417 kb on - strand, within kefBat 3477.506 kb on + strand, within kefBat 3477.515 kb on - strand, within kefBat 3477.574 kb on + strand, within kefBat 3477.579 kb on + strand, within kefBat 3477.617 kb on + strand, within kefBat 3477.617 kb on + strand, within kefBat 3477.634 kb on - strand, within kefBat 3477.636 kb on + strand, within kefBat 3477.668 kb on + strand, within kefBat 3477.678 kb on + strand, within kefBat 3477.688 kb on - strand, within kefBat 3477.724 kb on + strand, within kefBat 3477.727 kb on + strand, within kefBat 3477.735 kb on - strand, within kefBat 3477.752 kb on - strand, within kefBat 3477.757 kb on - strand, within kefBat 3477.757 kb on - strand, within kefBat 3477.757 kb on - strand, within kefBat 3477.762 kb on - strand, within kefBat 3477.764 kb on + strand, within kefBat 3477.764 kb on + strand, within kefBat 3477.764 kb on + strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.772 kb on - strand, within kefBat 3477.827 kb on + strand, within kefBat 3477.827 kb on + strand, within kefBat 3477.827 kb on + strand, within kefBat 3477.835 kb on + strand, within kefBat 3477.863 kb on - strand, within kefBat 3477.863 kb on - strand, within kefBat 3477.921 kb on + strand, within kefBat 3477.956 kb on + strand, within kefBat 3477.982 kb on + strand, within kefBat 3477.982 kb on + strand, within kefBat 3477.993 kb on + strand, within kefBat 3478.001 kb on - strand, within kefBat 3478.001 kb on - strand, within kefBat 3478.001 kb on - strand, within kefBat 3478.062 kb on - strand, within kefBat 3478.062 kb on - strand, within kefBat 3478.071 kb on + strand, within kefBat 3478.079 kb on - strand, within kefBat 3478.082 kb on + strand, within kefBat 3478.099 kb on - strand, within kefBat 3478.118 kb on + strand, within kefBat 3478.130 kb on + strand, within kefBat 3478.135 kb on + strand, within kefBat 3478.135 kb on + strand, within kefBat 3478.138 kb on - strand, within kefBat 3478.223 kb on + strand, within kefBat 3478.223 kb on + strand, within kefBat 3478.247 kb on + strand, within kefBat 3478.255 kb on - strand, within kefBat 3478.255 kb on - strand, within kefBat 3478.272 kb on - strand, within kefBat 3478.279 kb on - strand, within kefBat 3478.279 kb on - strand, within kefBat 3478.291 kb on - strand, within kefBat 3478.291 kb on - strand, within kefBat 3478.408 kb on - strand, within kefBat 3478.427 kb on - strand, within kefBat 3478.440 kb on - strand, within kefBat 3478.470 kb on - strandat 3478.494 kb on - strandat 3478.528 kb on + strandat 3478.547 kb on - strandat 3478.576 kb on + strandat 3478.578 kb on - strandat 3478.578 kb on - strandat 3478.637 kb on + strandat 3478.639 kb on + strandat 3478.639 kb on + strandat 3478.669 kb on + strandat 3478.680 kb on + strandat 3478.694 kb on + strand, within kefGat 3478.694 kb on + strand, within kefGat 3478.736 kb on + strand, within kefGat 3478.755 kb on + strand, within kefGat 3478.755 kb on + strand, within kefGat 3478.780 kb on + strand, within kefGat 3478.780 kb on + strand, within kefGat 3478.780 kb on - strand, within kefGat 3478.780 kb on - strand, within kefGat 3478.794 kb on + strand, within kefGat 3478.794 kb on + strand, within kefGat 3478.819 kb on + strand, within kefGat 3478.845 kb on + strand, within kefGat 3478.869 kb on - strand, within kefGat 3478.937 kb on - strand, within kefGat 3478.941 kb on + strand, within kefGat 3478.944 kb on + strand, within kefGat 3478.965 kb on + strand, within kefGat 3478.965 kb on + strand, within kefGat 3478.966 kb on + strand, within kefGat 3478.966 kb on + strand, within kefGat 3478.988 kb on + strand, within kefGat 3478.988 kb on + strand, within kefGat 3479.017 kb on - strand, within kefGat 3479.017 kb on - strand, within kefGat 3479.057 kb on + strand, within kefGat 3479.057 kb on + strand, within kefGat 3479.080 kb on + strand, within kefGat 3479.085 kb on + strand, within kefGat 3479.088 kb on - strand, within kefGat 3479.098 kb on + strand, within kefGat 3479.108 kb on + strand, within kefGat 3479.154 kb on - strandat 3479.170 kb on + strandat 3479.178 kb on - strandat 3479.178 kb on - strandat 3479.205 kb on + strandat 3479.205 kb on + strandat 3479.205 kb on + strandat 3479.305 kb on - strandat 3479.305 kb on - strandat 3479.437 kb on - strandat 3479.437 kb on - strandat 3479.445 kb on - strandat 3479.445 kb on - strandat 3479.486 kb on + strandat 3479.599 kb on + strand, within yheSat 3479.607 kb on - strand, within yheSat 3479.607 kb on - strand, within yheSat 3479.628 kb on - strand, within yheS

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM
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3,475,861 + +1.6
3,475,874 + -1.4
3,475,874 + +2.5
3,475,939 - -2.0
3,475,971 + -1.7
3,475,991 - slyD b3349 0.10 -1.6
3,475,991 - slyD b3349 0.10 -2.1
3,476,000 - slyD b3349 0.12 -1.8
3,476,042 + +0.2
3,476,042 + slyD b3349 0.19 -0.1
3,476,061 + slyD b3349 0.22 -1.6
3,476,061 + slyD b3349 0.22 -1.2
3,476,063 + slyD b3349 0.23 -1.9
3,476,063 + slyD b3349 0.23 +1.2
3,476,089 - slyD b3349 0.27 -1.9
3,476,089 - slyD b3349 0.27 -1.7
3,476,114 - slyD b3349 0.31 -0.6
3,476,143 + slyD b3349 0.36 -1.0
3,476,206 + slyD b3349 0.47 +0.2
3,476,212 - slyD b3349 0.48 -0.8
3,476,242 + slyD b3349 0.53 -1.7
3,476,286 + slyD b3349 0.60 -2.1
3,476,333 - slyD b3349 0.68 -1.7
3,476,378 - slyD b3349 0.76 -0.7
3,476,423 - slyD b3349 0.84 -0.9
3,476,423 - slyD b3349 0.84 -1.6
3,476,465 + +0.6
3,476,473 - -1.5
3,476,473 - -1.1
3,476,479 + -0.6
3,476,479 + +0.5
3,476,485 - -0.5
3,476,485 - -1.1
3,476,522 - -1.7
3,476,525 + -1.0
3,476,525 + -1.2
3,476,525 + -3.0
3,476,525 + -0.8
3,476,544 - -0.4
3,476,544 - +1.6
3,476,549 - +0.4
3,476,612 - +1.9
3,476,649 - yheV b4551 0.17 +1.1
3,476,768 - yheV b4551 0.77 -1.4
3,476,810 + +0.6
3,476,853 - +0.8
3,476,917 - +2.8
3,476,917 - +2.0
3,476,933 + +0.9
3,476,941 - -0.5
3,476,941 - +1.4
3,476,941 - +1.1
3,476,972 - +1.2
3,477,015 - kefB b3350 0.11 +0.7
3,477,021 - -0.4
3,477,047 - kefB b3350 0.12 +1.6
3,477,096 + kefB b3350 0.15 -0.2
3,477,096 + kefB b3350 0.15 +0.2
3,477,101 + kefB b3350 0.15 -0.8
3,477,101 + kefB b3350 0.15 +0.4
3,477,109 - kefB b3350 0.16 +2.1
3,477,109 - kefB b3350 0.16 +2.7
3,477,111 + kefB b3350 0.16 -2.4
3,477,111 + kefB b3350 0.16 +0.7
3,477,113 - kefB b3350 0.16 +2.6
3,477,113 - kefB b3350 0.16 +0.6
3,477,129 - +1.2
3,477,129 - kefB b3350 0.17 +1.9
3,477,131 + kefB b3350 0.17 +0.1
3,477,131 - kefB b3350 0.17 +2.6
3,477,131 - kefB b3350 0.17 +1.4
3,477,164 - kefB b3350 0.19 +1.0
3,477,164 - kefB b3350 0.19 -0.4
3,477,189 + kefB b3350 0.20 +0.5
3,477,189 + kefB b3350 0.20 +0.2
3,477,197 - kefB b3350 0.21 +1.2
3,477,197 - kefB b3350 0.21 +0.2
3,477,201 + kefB b3350 0.21 +0.3
3,477,218 - kefB b3350 0.22 +1.4
3,477,219 - kefB b3350 0.22 +0.9
3,477,256 + kefB b3350 0.24 +0.4
3,477,256 + kefB b3350 0.24 +0.4
3,477,271 - kefB b3350 0.25 +0.6
3,477,271 - kefB b3350 0.25 +0.7
3,477,313 - kefB b3350 0.27 +0.6
3,477,313 - kefB b3350 0.27 +1.4
3,477,326 + kefB b3350 0.28 -0.4
3,477,337 - kefB b3350 0.28 +1.6
3,477,337 - kefB b3350 0.28 +1.6
3,477,350 - kefB b3350 0.29 +0.8
3,477,388 + kefB b3350 0.31 -0.2
3,477,417 - kefB b3350 0.33 +1.6
3,477,506 + kefB b3350 0.38 -0.8
3,477,515 - kefB b3350 0.38 +0.8
3,477,574 + kefB b3350 0.42 -0.1
3,477,579 + kefB b3350 0.42 +0.2
3,477,617 + kefB b3350 0.44 +0.0
3,477,617 + kefB b3350 0.44 +0.3
3,477,634 - kefB b3350 0.45 +1.0
3,477,636 + kefB b3350 0.45 -0.1
3,477,668 + kefB b3350 0.47 +1.1
3,477,678 + kefB b3350 0.47 +0.3
3,477,688 - kefB b3350 0.48 +1.0
3,477,724 + kefB b3350 0.50 +0.4
3,477,727 + kefB b3350 0.50 -0.5
3,477,735 - kefB b3350 0.50 +0.9
3,477,752 - kefB b3350 0.51 +0.8
3,477,757 - kefB b3350 0.52 +1.1
3,477,757 - kefB b3350 0.52 +1.2
3,477,757 - kefB b3350 0.52 -1.1
3,477,762 - kefB b3350 0.52 +0.9
3,477,764 + kefB b3350 0.52 +1.2
3,477,764 + kefB b3350 0.52 +1.0
3,477,764 + kefB b3350 0.52 +1.0
3,477,772 - kefB b3350 0.52 +0.1
3,477,772 - kefB b3350 0.52 -0.2
3,477,772 - kefB b3350 0.52 +1.2
3,477,772 - kefB b3350 0.52 +0.1
3,477,827 + kefB b3350 0.56 -0.6
3,477,827 + kefB b3350 0.56 +0.7
3,477,827 + kefB b3350 0.56 +0.4
3,477,835 + kefB b3350 0.56 +2.0
3,477,863 - kefB b3350 0.58 +1.1
3,477,863 - kefB b3350 0.58 +0.8
3,477,921 + kefB b3350 0.61 +0.2
3,477,956 + kefB b3350 0.63 -0.9
3,477,982 + kefB b3350 0.64 +1.6
3,477,982 + kefB b3350 0.64 -0.4
3,477,993 + kefB b3350 0.65 +0.6
3,478,001 - kefB b3350 0.65 +0.8
3,478,001 - kefB b3350 0.65 +1.7
3,478,001 - kefB b3350 0.65 +0.4
3,478,062 - kefB b3350 0.69 +0.6
3,478,062 - kefB b3350 0.69 +1.8
3,478,071 + kefB b3350 0.69 -0.0
3,478,079 - kefB b3350 0.69 +0.8
3,478,082 + kefB b3350 0.70 +0.0
3,478,099 - kefB b3350 0.71 +0.6
3,478,118 + kefB b3350 0.72 +1.5
3,478,130 + kefB b3350 0.72 +0.9
3,478,135 + kefB b3350 0.73 -1.9
3,478,135 + kefB b3350 0.73 -0.4
3,478,138 - kefB b3350 0.73 -4.8
3,478,223 + kefB b3350 0.77 +1.5
3,478,223 + kefB b3350 0.77 -0.9
3,478,247 + kefB b3350 0.79 +0.1
3,478,255 - kefB b3350 0.79 +2.0
3,478,255 - kefB b3350 0.79 +0.2
3,478,272 - kefB b3350 0.80 +0.4
3,478,279 - kefB b3350 0.81 +0.6
3,478,279 - kefB b3350 0.81 +1.9
3,478,291 - kefB b3350 0.81 +0.2
3,478,291 - kefB b3350 0.81 +0.7
3,478,408 - kefB b3350 0.88 +0.9
3,478,427 - kefB b3350 0.89 -0.6
3,478,440 - kefB b3350 0.89 +0.6
3,478,470 - +0.5
3,478,494 - +1.0
3,478,528 + +0.6
3,478,547 - +0.8
3,478,576 + -0.3
3,478,578 - +0.6
3,478,578 - +1.0
3,478,637 + -0.5
3,478,639 + +1.6
3,478,639 + +0.1
3,478,669 + +0.3
3,478,680 + +0.2
3,478,694 + kefG b3351 0.12 -0.7
3,478,694 + kefG b3351 0.12 -0.1
3,478,736 + kefG b3351 0.19 +0.3
3,478,755 + kefG b3351 0.23 -1.8
3,478,755 + kefG b3351 0.23 +0.4
3,478,780 + kefG b3351 0.27 -0.4
3,478,780 + kefG b3351 0.27 +1.6
3,478,780 - kefG b3351 0.27 +0.4
3,478,780 - kefG b3351 0.27 -1.6
3,478,794 + kefG b3351 0.30 -0.5
3,478,794 + kefG b3351 0.30 +0.1
3,478,819 + kefG b3351 0.34 +0.5
3,478,845 + kefG b3351 0.39 -0.6
3,478,869 - kefG b3351 0.43 -0.1
3,478,937 - kefG b3351 0.55 +0.7
3,478,941 + kefG b3351 0.56 -0.3
3,478,944 + kefG b3351 0.57 +1.2
3,478,965 + kefG b3351 0.61 +0.6
3,478,965 + kefG b3351 0.61 +0.4
3,478,966 + kefG b3351 0.61 -1.0
3,478,966 + kefG b3351 0.61 -0.4
3,478,988 + kefG b3351 0.65 +1.6
3,478,988 + kefG b3351 0.65 -1.1
3,479,017 - kefG b3351 0.70 -2.0
3,479,017 - kefG b3351 0.70 -3.2
3,479,057 + kefG b3351 0.77 -1.4
3,479,057 + kefG b3351 0.77 -0.6
3,479,080 + kefG b3351 0.81 -0.7
3,479,085 + kefG b3351 0.82 -0.6
3,479,088 - kefG b3351 0.83 -0.4
3,479,098 + kefG b3351 0.85 +0.2
3,479,108 + kefG b3351 0.86 -1.7
3,479,154 - -0.6
3,479,170 + -0.4
3,479,178 - +1.0
3,479,178 - +1.0
3,479,205 + -0.4
3,479,205 + -0.6
3,479,205 + -0.8
3,479,305 - -0.2
3,479,305 - +0.1
3,479,437 - +1.0
3,479,437 - +1.2
3,479,445 - +0.0
3,479,445 - +0.0
3,479,486 + +0.5
3,479,599 + yheS b3352 0.15 +0.4
3,479,607 - yheS b3352 0.15 +0.5
3,479,607 - yheS b3352 0.15 -0.9
3,479,628 - yheS b3352 0.17 -0.5

Or see this region's nucleotide sequence