Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aas and rygA are separated by 87 nucleotides rygA and omrB are separated by 114 nucleotides omrB and galR are separated by 213 nucleotides galR and lysA are separated by 6 nucleotides
b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036
aas
b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211
rygA
b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407
omrB
b2837: galR - DNA-binding transcriptional repressor (NCBI), at 2,974,621 to 2,975,652
galR
b2838: lysA - diaminopimelate decarboxylase, PLP-binding (NCBI), at 2,975,659 to 2,976,921
lysA
Position (kb)
2974
2975
2976 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2973.699 kb on - strand, within aas at 2973.741 kb on + strand, within aas at 2973.754 kb on + strand, within aas at 2973.831 kb on - strand at 2973.831 kb on - strand at 2973.842 kb on - strand at 2973.894 kb on - strand at 2973.916 kb on + strand at 2973.918 kb on + strand at 2973.974 kb on - strand at 2973.995 kb on + strand at 2974.053 kb on - strand at 2974.055 kb on + strand at 2974.055 kb on + strand at 2974.090 kb on + strand at 2974.090 kb on + strand at 2974.097 kb on - strand at 2974.148 kb on + strand, within rygA at 2974.161 kb on - strand, within rygA at 2974.163 kb on - strand, within rygA at 2974.163 kb on - strand, within rygA at 2974.168 kb on - strand, within rygA at 2974.174 kb on - strand, within rygA at 2974.183 kb on - strand, within rygA at 2974.340 kb on + strand, within omrB at 2974.353 kb on - strand, within omrB at 2974.363 kb on - strand, within omrB at 2974.381 kb on - strand, within omrB at 2974.429 kb on - strand at 2974.581 kb on - strand at 2974.635 kb on + strand at 2974.635 kb on + strand at 2974.636 kb on - strand at 2974.636 kb on - strand at 2974.684 kb on + strand at 2974.684 kb on + strand at 2974.723 kb on + strand at 2974.733 kb on + strand, within galR at 2974.733 kb on + strand, within galR at 2974.741 kb on - strand, within galR at 2974.741 kb on - strand, within galR at 2974.751 kb on + strand, within galR at 2974.793 kb on + strand, within galR at 2974.793 kb on + strand, within galR at 2975.062 kb on - strand, within galR at 2975.089 kb on + strand, within galR at 2975.111 kb on - strand, within galR at 2975.111 kb on - strand, within galR at 2975.152 kb on - strand, within galR at 2975.168 kb on + strand, within galR at 2975.176 kb on - strand, within galR at 2975.348 kb on + strand, within galR at 2975.354 kb on - strand, within galR at 2975.357 kb on + strand, within galR at 2975.399 kb on + strand, within galR at 2975.399 kb on + strand, within galR at 2975.406 kb on - strand, within galR at 2975.406 kb on - strand, within galR at 2975.407 kb on - strand, within galR at 2975.457 kb on + strand, within galR at 2975.457 kb on + strand, within galR at 2975.478 kb on - strand, within galR at 2975.516 kb on - strand, within galR at 2975.627 kb on - strand at 2975.794 kb on - strand, within lysA at 2975.794 kb on - strand, within lysA at 2975.806 kb on - strand, within lysA at 2975.816 kb on - strand, within lysA at 2975.871 kb on + strand, within lysA at 2975.883 kb on - strand, within lysA at 2975.917 kb on + strand, within lysA at 2975.984 kb on + strand, within lysA at 2976.036 kb on - strand, within lysA at 2976.036 kb on - strand, within lysA at 2976.041 kb on - strand, within lysA at 2976.216 kb on - strand, within lysA at 2976.302 kb on + strand, within lysA at 2976.305 kb on + strand, within lysA at 2976.305 kb on + strand, within lysA at 2976.305 kb on - strand, within lysA at 2976.305 kb on - strand, within lysA at 2976.313 kb on - strand, within lysA at 2976.319 kb on - strand, within lysA at 2976.345 kb on - strand, within lysA at 2976.397 kb on - strand, within lysA at 2976.401 kb on + strand, within lysA at 2976.420 kb on + strand, within lysA at 2976.424 kb on - strand, within lysA at 2976.424 kb on - strand, within lysA at 2976.428 kb on - strand, within lysA at 2976.428 kb on - strand, within lysA at 2976.479 kb on - strand, within lysA at 2976.494 kb on + strand, within lysA at 2976.501 kb on + strand, within lysA at 2976.501 kb on + strand, within lysA at 2976.532 kb on - strand, within lysA at 2976.532 kb on - strand, within lysA at 2976.554 kb on - strand, within lysA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM remove 2,973,699 - aas b2836 0.84 +0.2 2,973,741 + aas b2836 0.86 +0.0 2,973,754 + aas b2836 0.87 +0.1 2,973,831 - +0.5 2,973,831 - +0.5 2,973,842 - -0.4 2,973,894 - +0.8 2,973,916 + +0.7 2,973,918 + +0.0 2,973,974 - +0.9 2,973,995 + +0.5 2,974,053 - -1.8 2,974,055 + -0.1 2,974,055 + +0.9 2,974,090 + +0.2 2,974,090 + +0.9 2,974,097 - -1.3 2,974,148 + rygA b4444 0.27 -0.1 2,974,161 - rygA b4444 0.42 -0.1 2,974,163 - rygA b4444 0.44 +1.7 2,974,163 - rygA b4444 0.44 +0.0 2,974,168 - rygA b4444 0.50 -0.8 2,974,174 - rygA b4444 0.57 -0.2 2,974,183 - rygA b4444 0.67 +0.3 2,974,340 + omrB b4445 0.17 +1.3 2,974,353 - omrB b4445 0.33 +0.4 2,974,363 - omrB b4445 0.45 +0.3 2,974,381 - omrB b4445 0.67 +0.0 2,974,429 - -0.2 2,974,581 - +1.5 2,974,635 + +2.2 2,974,635 + +2.6 2,974,636 - -1.5 2,974,636 - +0.6 2,974,684 + +0.2 2,974,684 + +1.6 2,974,723 + +0.4 2,974,733 + galR b2837 0.11 +3.9 2,974,733 + galR b2837 0.11 +1.9 2,974,741 - galR b2837 0.12 +0.4 2,974,741 - galR b2837 0.12 +1.0 2,974,751 + galR b2837 0.13 +1.0 2,974,793 + galR b2837 0.17 +1.5 2,974,793 + galR b2837 0.17 +0.6 2,975,062 - galR b2837 0.43 +1.5 2,975,089 + galR b2837 0.45 +0.8 2,975,111 - galR b2837 0.47 +1.4 2,975,111 - galR b2837 0.47 +3.2 2,975,152 - galR b2837 0.51 +0.1 2,975,168 + galR b2837 0.53 +1.4 2,975,176 - galR b2837 0.54 +0.7 2,975,348 + galR b2837 0.70 +0.9 2,975,354 - galR b2837 0.71 +1.8 2,975,357 + galR b2837 0.71 +1.5 2,975,399 + galR b2837 0.75 +0.6 2,975,399 + galR b2837 0.75 +0.9 2,975,406 - galR b2837 0.76 +1.9 2,975,406 - galR b2837 0.76 +2.3 2,975,407 - galR b2837 0.76 +1.2 2,975,457 + galR b2837 0.81 +1.3 2,975,457 + galR b2837 0.81 +0.4 2,975,478 - galR b2837 0.83 +1.3 2,975,516 - galR b2837 0.87 +2.1 2,975,627 - +0.3 2,975,794 - lysA b2838 0.11 +0.1 2,975,794 - lysA b2838 0.11 +1.1 2,975,806 - lysA b2838 0.12 -0.1 2,975,816 - lysA b2838 0.12 +0.6 2,975,871 + lysA b2838 0.17 -1.5 2,975,883 - lysA b2838 0.18 +2.2 2,975,917 + lysA b2838 0.20 +0.5 2,975,984 + lysA b2838 0.26 +0.3 2,976,036 - lysA b2838 0.30 +1.4 2,976,036 - lysA b2838 0.30 -0.7 2,976,041 - lysA b2838 0.30 -0.7 2,976,216 - lysA b2838 0.44 +0.4 2,976,302 + lysA b2838 0.51 +1.5 2,976,305 + lysA b2838 0.51 -0.1 2,976,305 + lysA b2838 0.51 +2.3 2,976,305 - lysA b2838 0.51 -0.4 2,976,305 - lysA b2838 0.51 -0.1 2,976,313 - lysA b2838 0.52 +0.3 2,976,319 - lysA b2838 0.52 +0.2 2,976,345 - lysA b2838 0.54 +0.9 2,976,397 - lysA b2838 0.58 +0.2 2,976,401 + lysA b2838 0.59 -0.4 2,976,420 + lysA b2838 0.60 +0.6 2,976,424 - lysA b2838 0.61 -1.1 2,976,424 - lysA b2838 0.61 -0.2 2,976,428 - lysA b2838 0.61 +0.1 2,976,428 - lysA b2838 0.61 -0.8 2,976,479 - lysA b2838 0.65 -0.5 2,976,494 + lysA b2838 0.66 -0.2 2,976,501 + lysA b2838 0.67 +0.1 2,976,501 + lysA b2838 0.67 +0.4 2,976,532 - lysA b2838 0.69 -0.2 2,976,532 - lysA b2838 0.69 -0.8 2,976,554 - lysA b2838 0.71 -0.4
Or see this region's nucleotide sequence