Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruA and fruK are separated by 16 nucleotides fruK and fruB overlap by 1 nucleotides fruB and setB are separated by 367 nucleotides
b2167: fruA - fused fructose-specific PTS enzymes: IIBcomponent/IIC components (NCBI), at 2,257,741 to 2,259,432
fruA
b2168: fruK - 1-phosphofructokinase (NCBI), at 2,259,449 to 2,260,387
fruK
b2169: fruB - fused fructose-specific PTS enzymes: IIA component/HPr component (NCBI), at 2,260,387 to 2,261,517
fruB
b2170: setB - lactose/glucose efflux system (NCBI), at 2,261,885 to 2,263,066
setB
Position (kb)
2260
2261
2262 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2259.507 kb on + strand at 2259.951 kb on - strand, within fruK at 2260.134 kb on + strand, within fruK at 2260.153 kb on + strand, within fruK at 2260.398 kb on + strand at 2260.398 kb on + strand at 2260.412 kb on - strand at 2260.416 kb on - strand at 2260.592 kb on + strand, within fruB at 2260.635 kb on - strand, within fruB at 2260.821 kb on - strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.911 kb on + strand, within fruB at 2260.938 kb on + strand, within fruB at 2261.006 kb on - strand, within fruB at 2261.202 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.241 kb on + strand, within fruB at 2261.255 kb on + strand, within fruB at 2261.330 kb on - strand, within fruB at 2261.476 kb on - strand at 2261.937 kb on - strand at 2262.160 kb on - strand, within setB at 2262.373 kb on - strand, within setB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM remove 2,259,507 + -0.4 2,259,951 - fruK b2168 0.53 -2.6 2,260,134 + fruK b2168 0.73 -1.0 2,260,153 + fruK b2168 0.75 -0.4 2,260,398 + -0.2 2,260,398 + +0.8 2,260,412 - +0.6 2,260,416 - +0.3 2,260,592 + fruB b2169 0.18 +0.6 2,260,635 - fruB b2169 0.22 -0.4 2,260,821 - fruB b2169 0.38 +0.5 2,260,911 + fruB b2169 0.46 +1.2 2,260,911 + fruB b2169 0.46 -0.4 2,260,938 + fruB b2169 0.49 +1.1 2,261,006 - fruB b2169 0.55 +0.0 2,261,202 + fruB b2169 0.72 +2.0 2,261,241 + fruB b2169 0.76 +1.1 2,261,241 + fruB b2169 0.76 +0.9 2,261,255 + fruB b2169 0.77 +0.7 2,261,330 - fruB b2169 0.83 +0.1 2,261,476 - +0.6 2,261,937 - -0.3 2,262,160 - setB b2170 0.23 -0.9 2,262,373 - setB b2170 0.41 +0.2
Or see this region's nucleotide sequence