Strain Fitness in Escherichia coli BW25113 around b1300

Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjL and puuR are separated by 26 nucleotidespuuR and puuC are separated by 274 nucleotidespuuC and puuB are separated by 1 nucleotides b1298: ycjL - probable amidotransferase subunit (VIMSS), at 1,359,144 to 1,359,908 ycjL b1299: puuR - DNA-binding transcriptional repressor (NCBI), at 1,359,935 to 1,360,492 puuR b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254 puuC b1301: puuB - gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI), at 1,362,256 to 1,363,536 puuB Position (kb) 1360 1361 1362 1363Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6at 1360.081 kb on - strand, within puuRat 1360.084 kb on - strand, within puuRat 1360.317 kb on - strand, within puuRat 1360.332 kb on - strand, within puuRat 1360.334 kb on + strand, within puuRat 1360.413 kb on - strand, within puuRat 1360.478 kb on - strandat 1360.478 kb on - strandat 1360.521 kb on - strandat 1360.526 kb on - strandat 1360.526 kb on - strandat 1360.698 kb on - strandat 1360.698 kb on - strandat 1360.761 kb on - strandat 1360.903 kb on - strandat 1360.903 kb on - strandat 1360.933 kb on - strand, within puuCat 1361.146 kb on + strand, within puuCat 1361.146 kb on + strand, within puuCat 1361.262 kb on - strand, within puuCat 1361.563 kb on - strand, within puuCat 1361.674 kb on - strand, within puuCat 1361.727 kb on - strand, within puuCat 1362.160 kb on + strandat 1362.229 kb on - strandat 1362.317 kb on + strandat 1362.520 kb on + strand, within puuBat 1362.664 kb on + strand, within puuBat 1362.716 kb on - strand, within puuBat 1362.757 kb on + strand, within puuB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM
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1,360,081 - puuR b1299 0.26 +1.6
1,360,084 - puuR b1299 0.27 +0.7
1,360,317 - puuR b1299 0.68 +0.5
1,360,332 - puuR b1299 0.71 +1.4
1,360,334 + puuR b1299 0.72 +0.3
1,360,413 - puuR b1299 0.86 +0.4
1,360,478 - -0.5
1,360,478 - +0.0
1,360,521 - +0.2
1,360,526 - -0.2
1,360,526 - -0.6
1,360,698 - +0.7
1,360,698 - -1.1
1,360,761 - -0.9
1,360,903 - +0.4
1,360,903 - -0.0
1,360,933 - puuC b1300 0.11 +0.7
1,361,146 + puuC b1300 0.25 +0.4
1,361,146 + puuC b1300 0.25 -1.2
1,361,262 - puuC b1300 0.33 +0.0
1,361,563 - puuC b1300 0.53 +0.4
1,361,674 - puuC b1300 0.61 +0.3
1,361,727 - puuC b1300 0.65 +0.3
1,362,160 + -0.0
1,362,229 - -0.4
1,362,317 + +0.8
1,362,520 + puuB b1301 0.21 -0.0
1,362,664 + puuB b1301 0.32 +5.9
1,362,716 - puuB b1301 0.36 +1.0
1,362,757 + puuB b1301 0.39 -0.8

Or see this region's nucleotide sequence