Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0641 and PP_5452 are separated by 982 nucleotides PP_5452 and PP_0642 overlap by 11 nucleotides PP_0642 and PP_0643 are separated by 31 nucleotides PP_0643 and PP_0645 are separated by 167 nucleotides
PP_0641: PP_0641 - conserved protein of unknown function, at 752,456 to 752,761
_0641
PP_5452: PP_5452 - protein of unknown function, at 753,744 to 753,926
_5452
PP_0642: PP_0642 - conserved membrane protein of unknown function, at 753,916 to 754,530
_0642
PP_0643: PP_0643 - protein of unknown function, at 754,562 to 754,744
_0643
PP_0645: PP_0645 - protein of unknown function, at 754,912 to 755,154
_0645
Position (kb) 753
754 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 752.745 kb on - strand at 752.849 kb on + strand at 752.850 kb on - strand at 752.850 kb on - strand at 752.942 kb on + strand at 752.976 kb on + strand at 752.976 kb on + strand at 752.977 kb on - strand at 752.977 kb on - strand at 753.005 kb on + strand at 753.005 kb on + strand at 753.005 kb on + strand at 753.006 kb on - strand at 753.009 kb on + strand at 753.009 kb on + strand at 753.071 kb on + strand at 753.071 kb on + strand at 753.072 kb on - strand at 753.206 kb on + strand at 753.207 kb on - strand at 753.207 kb on - strand at 753.207 kb on - strand at 753.216 kb on - strand at 753.222 kb on + strand at 753.223 kb on - strand at 753.228 kb on - strand at 753.231 kb on + strand at 753.231 kb on + strand at 753.231 kb on + strand at 753.232 kb on - strand at 753.238 kb on + strand at 753.239 kb on - strand at 753.241 kb on + strand at 753.241 kb on + strand at 753.251 kb on + strand at 753.409 kb on + strand at 753.436 kb on + strand at 753.436 kb on + strand at 753.437 kb on - strand at 753.437 kb on - strand at 753.437 kb on - strand at 753.440 kb on + strand at 753.440 kb on + strand at 753.440 kb on + strand at 753.441 kb on - strand at 753.465 kb on + strand at 753.466 kb on - strand at 753.518 kb on - strand at 753.624 kb on - strand at 753.759 kb on + strand at 753.813 kb on + strand, within PP_5452 at 753.813 kb on + strand, within PP_5452 at 753.813 kb on + strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.814 kb on - strand, within PP_5452 at 753.827 kb on + strand, within PP_5452 at 753.827 kb on + strand, within PP_5452 at 753.915 kb on + strand at 753.915 kb on + strand at 753.915 kb on + strand at 753.941 kb on - strand at 753.959 kb on + strand at 753.959 kb on + strand at 753.959 kb on + strand at 754.210 kb on - strand, within PP_0642 at 754.229 kb on + strand, within PP_0642 at 754.432 kb on + strand, within PP_0642 at 754.432 kb on + strand, within PP_0642 at 754.432 kb on + strand, within PP_0642 at 754.433 kb on - strand, within PP_0642 at 754.664 kb on + strand, within PP_0643 at 754.664 kb on + strand, within PP_0643 at 754.665 kb on - strand, within PP_0643 at 754.856 kb on + strand at 754.857 kb on - strand at 754.857 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 752,745 - +0.5 752,849 + -1.5 752,850 - -1.2 752,850 - -1.9 752,942 + +3.0 752,976 + -0.6 752,976 + +2.0 752,977 - -1.1 752,977 - +0.4 753,005 + -0.1 753,005 + -1.0 753,005 + -1.2 753,006 - -1.4 753,009 + -0.7 753,009 + -2.4 753,071 + -0.8 753,071 + -0.6 753,072 - -0.4 753,206 + -0.0 753,207 - +0.1 753,207 - -0.4 753,207 - -0.3 753,216 - -0.6 753,222 + -0.2 753,223 - -2.1 753,228 - -2.0 753,231 + +1.2 753,231 + -1.2 753,231 + +0.1 753,232 - -1.2 753,238 + +1.0 753,239 - -0.1 753,241 + -0.5 753,241 + +1.3 753,251 + -0.6 753,409 + -1.6 753,436 + -0.6 753,436 + +0.8 753,437 - -0.3 753,437 - -0.0 753,437 - +1.0 753,440 + +0.1 753,440 + +0.3 753,440 + +0.6 753,441 - +1.2 753,465 + +4.0 753,466 - +1.2 753,518 - -0.1 753,624 - -1.6 753,759 + -1.1 753,813 + PP_5452 0.38 +1.7 753,813 + PP_5452 0.38 -1.2 753,813 + PP_5452 0.38 -1.6 753,814 - PP_5452 0.38 -0.8 753,814 - PP_5452 0.38 +0.2 753,814 - PP_5452 0.38 -0.3 753,814 - PP_5452 0.38 -1.9 753,814 - PP_5452 0.38 -1.1 753,814 - PP_5452 0.38 -0.2 753,814 - PP_5452 0.38 +0.3 753,827 + PP_5452 0.45 -2.0 753,827 + PP_5452 0.45 -0.6 753,915 + -0.8 753,915 + -0.6 753,915 + -0.1 753,941 - +1.8 753,959 + -0.9 753,959 + +0.7 753,959 + -0.1 754,210 - PP_0642 0.48 -2.1 754,229 + PP_0642 0.51 +1.5 754,432 + PP_0642 0.84 -2.3 754,432 + PP_0642 0.84 -1.8 754,432 + PP_0642 0.84 +1.4 754,433 - PP_0642 0.84 -1.3 754,664 + PP_0643 0.56 -0.8 754,664 + PP_0643 0.56 -1.0 754,665 - PP_0643 0.56 +0.7 754,856 + -0.5 754,857 - -0.7 754,857 - -0.5
Or see this region's nucleotide sequence