Strain Fitness in Pseudomonas putida KT2440 around PP_5425

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
80,341	+				+0.2
80,341	+				-2.0
80,345	+				+1.1
80,345	+				-0.1
80,346	-				+0.2
80,346	-				-0.7
80,346	-				+0.2
80,365	+				+0.3
80,944	+				-0.5
80,944	+				+0.3
80,944	+				-0.0
80,944	+				+0.6
80,944	+				-0.5
80,945	-				+0.1
80,945	-				-1.2
81,007	+				-0.4
81,007	+				+1.0
81,007	+				+0.1
81,116	+		PP_5425	0.19	-0.2
81,116	+		PP_5425	0.19	-1.8
81,116	+		PP_5425	0.19	-0.9
81,117	-		PP_5425	0.20	+0.0
81,183	-		PP_5425	0.53	-1.0
81,183	-		PP_5425	0.53	+0.1
81,205	+		PP_5425	0.64	-0.8
81,206	-		PP_5425	0.64	-1.4
81,206	-		PP_5425	0.64	-2.1
81,244	-		PP_5425	0.83	-0.3
81,244	-		PP_5425	0.83	-1.2
81,270	+				+0.3
81,271	-				-0.5
81,318	+				-0.7
81,334	+				-0.3
81,334	+				-0.1
81,368	+				+0.5
81,369	-				-0.5
81,369	-				-0.2
81,369	-				+1.0
81,391	+				-0.6
81,392	-				+0.4
81,451	+				+1.1
81,451	+				+0.8
81,452	-				+2.9
81,456	+				-0.8
81,457	-				+0.5
81,457	-				-1.5
81,505	+				-1.0
81,506	-				+0.6
81,548	-				-0.7
81,618	+				-0.5
81,636	+				+3.6
81,648	+				+0.7
81,649	-				+0.3
81,649	-				+1.4
81,673	-				-0.6
81,704	+				+0.3
81,704	+				-0.6
81,704	+				-0.6
81,705	-				-0.4
81,705	-				+0.3
81,705	-				+0.0
81,705	-				+0.4
81,737	+		PP_0071	0.14	+0.6
81,839	+		PP_0071	0.44	+0.1
81,839	+		PP_0071	0.44	+0.8
81,840	-		PP_0071	0.45	+0.1
81,840	-		PP_0071	0.45	+0.3
81,845	+		PP_0071	0.46	+0.1
81,845	+		PP_0071	0.46	+1.3
81,846	-		PP_0071	0.47	-1.4
81,846	-		PP_0071	0.47	-1.2
81,847	+		PP_0071	0.47	+0.7
81,848	-		PP_0071	0.47	-0.4
81,848	-		PP_0071	0.47	-0.4
81,848	-		PP_0071	0.47	-0.1
81,848	-		PP_0071	0.47	-0.4
81,848	-		PP_0071	0.47	+0.6
81,862	+		PP_0071	0.51	+0.2
81,969	-		PP_0071	0.83	-0.8
82,022	-				+0.9
82,047	+				+0.2
82,047	+				+0.5
82,120	+				+0.5
82,132	+				-0.6
82,133	-				-0.4
82,163	+				+0.3
82,163	+				+0.3
82,164	-				-0.2