Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5247 and PP_5248 are separated by 348 nucleotides PP_5248 and PP_5249 are separated by 228 nucleotides
PP_5247: PP_5247 - conserved protein of unknown function, at 5,989,290 to 5,989,688
_5247
PP_5248: PP_5248 - putative hydrolase, at 5,990,037 to 5,990,666
_5248
PP_5249: PP_5249 - Transcriptional regulator, LysR family, at 5,990,895 to 5,991,806
_5249
Position (kb) 5990
5991 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 5989.076 kb on + strand at 5989.094 kb on + strand at 5989.095 kb on - strand at 5989.095 kb on - strand at 5989.141 kb on + strand at 5989.141 kb on + strand at 5989.141 kb on + strand at 5989.142 kb on - strand at 5989.145 kb on - strand at 5989.146 kb on + strand at 5989.146 kb on + strand at 5989.147 kb on - strand at 5989.195 kb on + strand at 5989.196 kb on - strand at 5989.264 kb on + strand at 5989.477 kb on + strand, within PP_5247 at 5989.478 kb on - strand, within PP_5247 at 5989.533 kb on + strand, within PP_5247 at 5989.534 kb on - strand, within PP_5247 at 5989.534 kb on - strand, within PP_5247 at 5989.534 kb on - strand, within PP_5247 at 5989.548 kb on + strand, within PP_5247 at 5989.549 kb on - strand, within PP_5247 at 5989.549 kb on - strand, within PP_5247 at 5989.560 kb on + strand, within PP_5247 at 5989.561 kb on - strand, within PP_5247 at 5989.757 kb on - strand at 5989.807 kb on + strand at 5989.807 kb on + strand at 5989.808 kb on - strand at 5989.808 kb on - strand at 5989.813 kb on + strand at 5989.813 kb on + strand at 5989.813 kb on + strand at 5989.813 kb on + strand at 5989.814 kb on - strand at 5989.814 kb on - strand at 5989.814 kb on - strand at 5989.814 kb on - strand at 5989.814 kb on - strand at 5989.851 kb on - strand at 5989.996 kb on + strand at 5989.997 kb on - strand at 5989.997 kb on - strand at 5990.038 kb on + strand at 5990.038 kb on + strand at 5990.038 kb on + strand at 5990.038 kb on + strand at 5990.038 kb on + strand at 5990.039 kb on - strand at 5990.039 kb on - strand at 5990.061 kb on - strand at 5990.061 kb on - strand at 5990.061 kb on - strand at 5990.062 kb on + strand at 5990.062 kb on + strand at 5990.062 kb on + strand at 5990.062 kb on + strand at 5990.063 kb on - strand at 5990.063 kb on - strand at 5990.277 kb on + strand at 5990.422 kb on + strand, within PP_5248 at 5990.422 kb on + strand, within PP_5248 at 5990.680 kb on - strand at 5990.687 kb on - strand at 5990.734 kb on + strand at 5990.758 kb on - strand at 5990.846 kb on + strand at 5990.846 kb on + strand at 5990.846 kb on + strand at 5990.847 kb on - strand at 5990.864 kb on + strand at 5990.916 kb on + strand at 5990.917 kb on - strand at 5990.919 kb on + strand at 5990.919 kb on + strand at 5990.919 kb on + strand at 5990.920 kb on - strand at 5990.920 kb on - strand at 5991.088 kb on - strand, within PP_5249 at 5991.088 kb on - strand, within PP_5249 at 5991.090 kb on + strand, within PP_5249 at 5991.090 kb on + strand, within PP_5249 at 5991.090 kb on + strand, within PP_5249 at 5991.091 kb on - strand, within PP_5249 at 5991.234 kb on + strand, within PP_5249 at 5991.234 kb on + strand, within PP_5249 at 5991.235 kb on - strand, within PP_5249 at 5991.235 kb on - strand, within PP_5249 at 5991.246 kb on + strand, within PP_5249 at 5991.247 kb on - strand, within PP_5249 at 5991.247 kb on - strand, within PP_5249 at 5991.276 kb on - strand, within PP_5249 at 5991.411 kb on + strand at 5991.412 kb on - strand, within PP_5249 at 5991.495 kb on + strand, within PP_5249 at 5991.551 kb on + strand, within PP_5249 at 5991.551 kb on + strand, within PP_5249 at 5991.552 kb on - strand, within PP_5249 at 5991.552 kb on - strand, within PP_5249
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,989,076 + +0.3 5,989,094 + +0.9 5,989,095 - +0.3 5,989,095 - -0.7 5,989,141 + +1.2 5,989,141 + +1.5 5,989,141 + +0.6 5,989,142 - -0.1 5,989,145 - -0.2 5,989,146 + +5.0 5,989,146 + +0.5 5,989,147 - -0.2 5,989,195 + +1.5 5,989,196 - -1.0 5,989,264 + -0.1 5,989,477 + PP_5247 0.47 -0.3 5,989,478 - PP_5247 0.47 +0.8 5,989,533 + PP_5247 0.61 -0.4 5,989,534 - PP_5247 0.61 -0.2 5,989,534 - PP_5247 0.61 -0.7 5,989,534 - PP_5247 0.61 -0.1 5,989,548 + PP_5247 0.65 -0.3 5,989,549 - PP_5247 0.65 -0.0 5,989,549 - PP_5247 0.65 +0.7 5,989,560 + PP_5247 0.68 +1.4 5,989,561 - PP_5247 0.68 +1.2 5,989,757 - +0.2 5,989,807 + +2.4 5,989,807 + -1.7 5,989,808 - +0.6 5,989,808 - -0.5 5,989,813 + +0.1 5,989,813 + -1.5 5,989,813 + -1.7 5,989,813 + -1.0 5,989,814 - +0.0 5,989,814 - +2.4 5,989,814 - -1.2 5,989,814 - +4.0 5,989,814 - -1.9 5,989,851 - -1.0 5,989,996 + -1.0 5,989,997 - -0.1 5,989,997 - +0.3 5,990,038 + -0.7 5,990,038 + +0.5 5,990,038 + +0.1 5,990,038 + -0.7 5,990,038 + -0.3 5,990,039 - -2.4 5,990,039 - -1.5 5,990,061 - -0.3 5,990,061 - -0.8 5,990,061 - -0.3 5,990,062 + +0.1 5,990,062 + +1.3 5,990,062 + -0.4 5,990,062 + +0.4 5,990,063 - +1.2 5,990,063 - +1.0 5,990,277 + +2.0 5,990,422 + PP_5248 0.61 -1.5 5,990,422 + PP_5248 0.61 +1.2 5,990,680 - -1.5 5,990,687 - -0.0 5,990,734 + -1.0 5,990,758 - -0.3 5,990,846 + -0.5 5,990,846 + -0.1 5,990,846 + -0.3 5,990,847 - +0.3 5,990,864 + -0.9 5,990,916 + -0.1 5,990,917 - +1.0 5,990,919 + +1.4 5,990,919 + -0.0 5,990,919 + -0.1 5,990,920 - -1.1 5,990,920 - -0.3 5,991,088 - PP_5249 0.21 +2.4 5,991,088 - PP_5249 0.21 -0.2 5,991,090 + PP_5249 0.21 +0.7 5,991,090 + PP_5249 0.21 +0.1 5,991,090 + PP_5249 0.21 -1.5 5,991,091 - PP_5249 0.21 +0.9 5,991,234 + PP_5249 0.37 -0.4 5,991,234 + PP_5249 0.37 +0.2 5,991,235 - PP_5249 0.37 -0.4 5,991,235 - PP_5249 0.37 +1.0 5,991,246 + PP_5249 0.38 -0.6 5,991,247 - PP_5249 0.39 +0.2 5,991,247 - PP_5249 0.39 +0.3 5,991,276 - PP_5249 0.42 -0.3 5,991,411 + +2.0 5,991,412 - PP_5249 0.57 +1.6 5,991,495 + PP_5249 0.66 +0.7 5,991,551 + PP_5249 0.72 +1.3 5,991,551 + PP_5249 0.72 +1.8 5,991,552 - PP_5249 0.72 +1.0 5,991,552 - PP_5249 0.72 +0.3
Or see this region's nucleotide sequence