Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_5139 and PP_5140 are separated by 83 nucleotides PP_5140 and PP_5141 are separated by 56 nucleotides PP_5141 and PP_5142 are separated by 51 nucleotides PP_5142 and PP_5143 are separated by 16 nucleotides
PP_5139: PP_5139 - cadmium translocating P-type ATPase, at 5,863,898 to 5,866,150
_5139
PP_5140: PP_5140 - Transcriptional regulator, MerR family, at 5,866,234 to 5,866,677
_5140
PP_5141: PP_5141 - Thymidylate synthase, at 5,866,734 to 5,867,705
_5141
PP_5142: PP_5142 - Prolipoprotein diacylglyceryl transferase, at 5,867,757 to 5,868,563
_5142
PP_5143: PP_5143 - conserved membrane protein of unknown function, at 5,868,580 to 5,869,362
_5143
Position (kb) 5866
5867
5868 Strain fitness (log2 ratio) -1
0
1
2 at 5866.203 kb on + strand at 5866.288 kb on + strand, within PP_5140 at 5866.288 kb on + strand, within PP_5140 at 5866.291 kb on + strand, within PP_5140 at 5866.292 kb on - strand, within PP_5140 at 5866.292 kb on - strand, within PP_5140 at 5866.339 kb on + strand at 5866.340 kb on - strand, within PP_5140 at 5866.349 kb on - strand, within PP_5140 at 5866.724 kb on + strand at 5866.725 kb on - strand at 5866.725 kb on - strand at 5866.732 kb on + strand at 5866.732 kb on + strand at 5866.732 kb on + strand at 5866.732 kb on + strand at 5867.775 kb on - strand at 5867.787 kb on - strand at 5867.787 kb on - strand at 5867.789 kb on - strand at 5867.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,866,203 + -0.6 5,866,288 + PP_5140 0.12 +0.4 5,866,288 + PP_5140 0.12 -0.5 5,866,291 + PP_5140 0.13 -1.2 5,866,292 - PP_5140 0.13 +0.5 5,866,292 - PP_5140 0.13 +1.0 5,866,339 + +1.2 5,866,340 - PP_5140 0.24 +0.1 5,866,349 - PP_5140 0.26 +1.5 5,866,724 + -1.1 5,866,725 - +0.2 5,866,725 - -0.1 5,866,732 + -1.0 5,866,732 + -1.2 5,866,732 + -0.2 5,866,732 + +0.4 5,867,775 - -1.1 5,867,787 - -0.2 5,867,787 - -0.2 5,867,789 - -1.0 5,867,789 - +2.2
Or see this region's nucleotide sequence