Strain Fitness in Pseudomonas putida KT2440 around PP_5110
Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 5,829,017 | - | -1.2 | |||
| 5,829,017 | - | +0.8 | |||
| 5,829,019 | - | -0.7 | |||
| 5,829,096 | + | PP_5109 | 0.13 | -0.3 | |
| 5,829,096 | + | PP_5109 | 0.13 | +0.3 | |
| 5,829,186 | + | PP_5109 | 0.22 | -0.1 | |
| 5,829,186 | + | PP_5109 | 0.22 | +0.5 | |
| 5,829,186 | + | PP_5109 | 0.22 | -0.0 | |
| 5,829,186 | + | PP_5109 | 0.22 | -0.2 | |
| 5,829,187 | - | PP_5109 | 0.22 | -1.6 | |
| 5,829,187 | - | PP_5109 | 0.22 | +1.7 | |
| 5,829,201 | + | PP_5109 | 0.23 | -0.5 | |
| 5,829,201 | + | PP_5109 | 0.23 | -1.2 | |
| 5,829,201 | + | PP_5109 | 0.23 | -0.8 | |
| 5,829,222 | + | PP_5109 | 0.25 | +2.4 | |
| 5,829,223 | - | PP_5109 | 0.25 | -0.7 | |
| 5,829,266 | - | PP_5109 | 0.30 | +0.4 | |
| 5,829,305 | + | PP_5109 | 0.33 | -0.1 | |
| 5,829,306 | - | PP_5109 | 0.33 | -2.3 | |
| 5,829,309 | - | PP_5109 | 0.34 | +0.9 | |
| 5,829,591 | + | PP_5109 | 0.61 | +0.1 | |
| 5,829,591 | + | PP_5109 | 0.61 | -0.8 | |
| 5,829,592 | - | PP_5109 | 0.61 | +4.0 | |
| 5,829,592 | - | PP_5109 | 0.61 | -0.6 | |
| 5,829,675 | + | PP_5109 | 0.69 | -0.1 | |
| 5,829,973 | + | +0.8 | |||
| 5,829,974 | - | -0.8 | |||
| 5,829,974 | - | -0.1 | |||
| 5,830,088 | - | PP_5110 | 0.15 | -0.3 | |
| 5,830,283 | - | PP_5110 | 0.44 | -1.9 |
Or see this region's nucleotide sequence