Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4756 and PP_4757 are separated by 560 nucleotides PP_4757 and PP_4758 are separated by 19 nucleotides
PP_4756: PP_4756 - gamma-aminobutyrate permease, at 5,414,385 to 5,415,776
_4756
PP_4757: PP_4757 - D-glucarate dehydratase / L-idarate epimerase, at 5,416,337 to 5,417,683
_4757
PP_4758: PP_4758 - Major facilitator family transporter, at 5,417,703 to 5,419,070
_4758
Position (kb) 5416
5417
5418 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 5415.456 kb on - strand, within PP_4756 at 5415.485 kb on - strand, within PP_4756 at 5415.500 kb on + strand, within PP_4756 at 5415.500 kb on + strand, within PP_4756 at 5415.500 kb on + strand, within PP_4756 at 5415.501 kb on - strand, within PP_4756 at 5415.501 kb on - strand, within PP_4756 at 5415.501 kb on - strand, within PP_4756 at 5415.501 kb on - strand, within PP_4756 at 5415.502 kb on + strand, within PP_4756 at 5415.535 kb on + strand, within PP_4756 at 5415.535 kb on + strand, within PP_4756 at 5415.536 kb on - strand, within PP_4756 at 5415.536 kb on - strand, within PP_4756 at 5415.601 kb on - strand, within PP_4756 at 5415.634 kb on - strand, within PP_4756 at 5415.634 kb on - strand, within PP_4756 at 5415.721 kb on - strand at 5415.823 kb on - strand at 5415.885 kb on - strand at 5415.935 kb on - strand at 5415.935 kb on - strand at 5415.959 kb on + strand at 5415.960 kb on - strand at 5416.032 kb on - strand at 5416.123 kb on + strand at 5416.124 kb on - strand at 5416.124 kb on - strand at 5416.269 kb on + strand at 5416.302 kb on + strand at 5416.302 kb on + strand at 5416.303 kb on - strand at 5416.438 kb on - strand at 5416.763 kb on + strand, within PP_4757 at 5416.764 kb on - strand, within PP_4757 at 5417.079 kb on + strand, within PP_4757 at 5417.217 kb on + strand, within PP_4757 at 5417.218 kb on - strand, within PP_4757 at 5417.290 kb on + strand, within PP_4757 at 5417.345 kb on + strand, within PP_4757 at 5417.345 kb on + strand, within PP_4757 at 5417.345 kb on + strand at 5417.345 kb on + strand, within PP_4757 at 5417.346 kb on - strand, within PP_4757 at 5417.346 kb on - strand, within PP_4757 at 5417.346 kb on - strand, within PP_4757 at 5417.346 kb on - strand, within PP_4757 at 5417.346 kb on - strand, within PP_4757 at 5417.527 kb on + strand, within PP_4757 at 5417.527 kb on + strand, within PP_4757 at 5417.677 kb on + strand at 5417.678 kb on - strand at 5417.822 kb on - strand at 5418.140 kb on - strand, within PP_4758 at 5418.140 kb on - strand, within PP_4758 at 5418.213 kb on - strand, within PP_4758 at 5418.250 kb on + strand at 5418.250 kb on + strand, within PP_4758 at 5418.250 kb on + strand, within PP_4758 at 5418.250 kb on + strand, within PP_4758 at 5418.250 kb on + strand, within PP_4758 at 5418.250 kb on + strand, within PP_4758 at 5418.251 kb on - strand, within PP_4758 at 5418.251 kb on - strand, within PP_4758 at 5418.251 kb on - strand, within PP_4758 at 5418.251 kb on - strand, within PP_4758 at 5418.251 kb on - strand, within PP_4758 at 5418.251 kb on - strand, within PP_4758 at 5418.274 kb on + strand at 5418.275 kb on - strand, within PP_4758 at 5418.275 kb on - strand, within PP_4758 at 5418.275 kb on - strand, within PP_4758 at 5418.404 kb on + strand, within PP_4758 at 5418.404 kb on + strand, within PP_4758 at 5418.405 kb on - strand, within PP_4758 at 5418.405 kb on - strand, within PP_4758 at 5418.643 kb on + strand, within PP_4758 at 5418.643 kb on + strand, within PP_4758
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 5,415,456 - PP_4756 0.77 -0.5 5,415,485 - PP_4756 0.79 +2.4 5,415,500 + PP_4756 0.80 -1.4 5,415,500 + PP_4756 0.80 -0.4 5,415,500 + PP_4756 0.80 -0.1 5,415,501 - PP_4756 0.80 +0.2 5,415,501 - PP_4756 0.80 +0.6 5,415,501 - PP_4756 0.80 -0.3 5,415,501 - PP_4756 0.80 -0.5 5,415,502 + PP_4756 0.80 -1.7 5,415,535 + PP_4756 0.83 +0.7 5,415,535 + PP_4756 0.83 +0.0 5,415,536 - PP_4756 0.83 -0.7 5,415,536 - PP_4756 0.83 +0.2 5,415,601 - PP_4756 0.87 -0.1 5,415,634 - PP_4756 0.90 -0.0 5,415,634 - PP_4756 0.90 -0.6 5,415,721 - -1.1 5,415,823 - -0.3 5,415,885 - +1.3 5,415,935 - -0.4 5,415,935 - +0.5 5,415,959 + -0.2 5,415,960 - -0.5 5,416,032 - +0.1 5,416,123 + -1.3 5,416,124 - -0.5 5,416,124 - +1.4 5,416,269 + -0.8 5,416,302 + -1.8 5,416,302 + -0.5 5,416,303 - -1.3 5,416,438 - -1.1 5,416,763 + PP_4757 0.32 +0.7 5,416,764 - PP_4757 0.32 -1.0 5,417,079 + PP_4757 0.55 -1.3 5,417,217 + PP_4757 0.65 -0.2 5,417,218 - PP_4757 0.65 -0.2 5,417,290 + PP_4757 0.71 -0.3 5,417,345 + PP_4757 0.75 -1.3 5,417,345 + PP_4757 0.75 -0.0 5,417,345 + +1.7 5,417,345 + PP_4757 0.75 +1.4 5,417,346 - PP_4757 0.75 -0.4 5,417,346 - PP_4757 0.75 -1.3 5,417,346 - PP_4757 0.75 +0.6 5,417,346 - PP_4757 0.75 -0.4 5,417,346 - PP_4757 0.75 +1.7 5,417,527 + PP_4757 0.88 +3.0 5,417,527 + PP_4757 0.88 -1.2 5,417,677 + +0.5 5,417,678 - -0.8 5,417,822 - -0.6 5,418,140 - PP_4758 0.32 -0.4 5,418,140 - PP_4758 0.32 +0.7 5,418,213 - PP_4758 0.37 +0.3 5,418,250 + +2.0 5,418,250 + PP_4758 0.40 -0.7 5,418,250 + PP_4758 0.40 +1.1 5,418,250 + PP_4758 0.40 +1.2 5,418,250 + PP_4758 0.40 +0.6 5,418,250 + PP_4758 0.40 +4.0 5,418,251 - PP_4758 0.40 -0.5 5,418,251 - PP_4758 0.40 -0.2 5,418,251 - PP_4758 0.40 -2.0 5,418,251 - PP_4758 0.40 -0.1 5,418,251 - PP_4758 0.40 -0.1 5,418,251 - PP_4758 0.40 +1.7 5,418,274 + +0.9 5,418,275 - PP_4758 0.42 +1.1 5,418,275 - PP_4758 0.42 +0.1 5,418,275 - PP_4758 0.42 +0.1 5,418,404 + PP_4758 0.51 +0.4 5,418,404 + PP_4758 0.51 -0.2 5,418,405 - PP_4758 0.51 -0.6 5,418,405 - PP_4758 0.51 -0.4 5,418,643 + PP_4758 0.69 -0.2 5,418,643 + PP_4758 0.69 -0.5
Or see this region's nucleotide sequence