Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4406 and PP_4407 are separated by 304 nucleotides
PP_4406: PP_4406 - conserved protein of unknown function, at 4,998,316 to 4,998,843
_4406
PP_4407: PP_4407 - putative Gamma-aminobutyrate transporter, at 4,999,148 to 5,000,530
_4407
Position (kb) 4999
5000
5001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4998.189 kb on + strand at 4998.204 kb on + strand at 4998.204 kb on + strand at 4998.204 kb on + strand at 4998.204 kb on + strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.205 kb on - strand at 4998.320 kb on + strand at 4998.320 kb on + strand at 4998.321 kb on - strand at 4998.370 kb on + strand, within PP_4406 at 4998.536 kb on + strand, within PP_4406 at 4998.682 kb on + strand, within PP_4406 at 4998.868 kb on + strand at 4998.868 kb on + strand at 4999.033 kb on + strand at 4999.033 kb on + strand at 4999.033 kb on + strand at 4999.034 kb on - strand at 4999.034 kb on - strand at 4999.034 kb on - strand at 4999.056 kb on + strand at 4999.056 kb on + strand at 4999.056 kb on + strand at 4999.056 kb on + strand at 4999.057 kb on - strand at 4999.060 kb on + strand at 4999.060 kb on + strand at 4999.061 kb on - strand at 4999.061 kb on - strand at 4999.061 kb on - strand at 4999.061 kb on - strand at 4999.189 kb on + strand at 4999.189 kb on + strand at 4999.190 kb on - strand at 4999.190 kb on - strand at 4999.190 kb on - strand at 4999.190 kb on - strand at 4999.278 kb on + strand at 4999.279 kb on - strand at 4999.286 kb on + strand at 4999.376 kb on + strand, within PP_4407 at 4999.389 kb on - strand, within PP_4407 at 4999.389 kb on - strand, within PP_4407 at 4999.389 kb on - strand, within PP_4407 at 4999.562 kb on + strand, within PP_4407 at 4999.587 kb on + strand, within PP_4407 at 4999.587 kb on + strand at 4999.592 kb on + strand, within PP_4407 at 4999.593 kb on - strand, within PP_4407 at 4999.593 kb on - strand, within PP_4407 at 4999.627 kb on + strand, within PP_4407 at 4999.627 kb on + strand, within PP_4407 at 4999.627 kb on + strand, within PP_4407 at 4999.628 kb on - strand, within PP_4407 at 4999.628 kb on - strand, within PP_4407 at 4999.628 kb on - strand, within PP_4407 at 4999.675 kb on + strand, within PP_4407 at 4999.676 kb on - strand, within PP_4407 at 4999.676 kb on - strand, within PP_4407 at 4999.676 kb on - strand, within PP_4407 at 4999.676 kb on - strand, within PP_4407 at 4999.676 kb on - strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.880 kb on + strand, within PP_4407 at 4999.881 kb on - strand, within PP_4407 at 4999.881 kb on - strand, within PP_4407 at 4999.881 kb on - strand, within PP_4407 at 4999.881 kb on - strand, within PP_4407 at 4999.881 kb on - strand, within PP_4407 at 4999.881 kb on - strand, within PP_4407 at 4999.884 kb on + strand, within PP_4407 at 4999.884 kb on + strand, within PP_4407 at 4999.884 kb on + strand, within PP_4407 at 4999.884 kb on + strand, within PP_4407 at 4999.884 kb on + strand, within PP_4407 at 4999.885 kb on - strand at 4999.885 kb on - strand, within PP_4407 at 4999.885 kb on - strand, within PP_4407 at 4999.885 kb on - strand, within PP_4407 at 4999.949 kb on + strand, within PP_4407 at 4999.949 kb on + strand, within PP_4407 at 4999.949 kb on + strand, within PP_4407 at 5000.130 kb on + strand, within PP_4407 at 5000.231 kb on - strand, within PP_4407 at 5000.253 kb on + strand, within PP_4407 at 5000.254 kb on - strand, within PP_4407 at 5000.254 kb on - strand, within PP_4407 at 5000.254 kb on - strand, within PP_4407 at 5000.348 kb on + strand, within PP_4407 at 5000.356 kb on + strand, within PP_4407 at 5000.356 kb on + strand, within PP_4407 at 5000.356 kb on + strand, within PP_4407 at 5000.357 kb on - strand, within PP_4407 at 5000.357 kb on - strand, within PP_4407 at 5000.357 kb on - strand, within PP_4407 at 5000.357 kb on - strand, within PP_4407 at 5000.448 kb on + strand at 5000.448 kb on + strand at 5000.449 kb on - strand at 5000.639 kb on + strand at 5000.640 kb on - strand at 5000.704 kb on + strand at 5000.704 kb on + strand at 5000.704 kb on + strand at 5000.704 kb on + strand at 5000.704 kb on + strand at 5000.705 kb on - strand at 5000.705 kb on - strand at 5000.705 kb on - strand at 5000.707 kb on + strand at 5000.718 kb on - strand at 5000.718 kb on - strand at 5000.820 kb on - strand at 5000.820 kb on - strand at 5000.820 kb on - strand at 5000.829 kb on - strand at 5000.878 kb on + strand at 5000.896 kb on - strand at 5000.896 kb on - strand at 5001.066 kb on + strand at 5001.066 kb on + strand at 5001.067 kb on - strand at 5001.100 kb on + strand at 5001.101 kb on - strand at 5001.101 kb on - strand at 5001.398 kb on + strand at 5001.466 kb on + strand at 5001.467 kb on - strand at 5001.467 kb on - strand at 5001.491 kb on + strand at 5001.491 kb on + strand at 5001.491 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,998,189 + +0.4 4,998,204 + -0.9 4,998,204 + -0.7 4,998,204 + -0.8 4,998,204 + +0.5 4,998,205 - +0.5 4,998,205 - -0.4 4,998,205 - +0.7 4,998,205 - -0.4 4,998,205 - +1.5 4,998,205 - +0.8 4,998,205 - +1.1 4,998,205 - -2.4 4,998,320 + +2.0 4,998,320 + -0.3 4,998,321 - +0.3 4,998,370 + PP_4406 0.10 +2.4 4,998,536 + PP_4406 0.42 -1.0 4,998,682 + PP_4406 0.69 -0.5 4,998,868 + -0.4 4,998,868 + -0.4 4,999,033 + -1.4 4,999,033 + +0.0 4,999,033 + +0.1 4,999,034 - -0.5 4,999,034 - +0.3 4,999,034 - -0.3 4,999,056 + -0.5 4,999,056 + +0.4 4,999,056 + +0.0 4,999,056 + -0.1 4,999,057 - -0.3 4,999,060 + -0.1 4,999,060 + -0.5 4,999,061 - +0.6 4,999,061 - +0.5 4,999,061 - -0.3 4,999,061 - -0.4 4,999,189 + -1.3 4,999,189 + +0.8 4,999,190 - +0.3 4,999,190 - +1.9 4,999,190 - +0.2 4,999,190 - -2.5 4,999,278 + -0.6 4,999,279 - +2.7 4,999,286 + +0.9 4,999,376 + PP_4407 0.16 -0.6 4,999,389 - PP_4407 0.17 -2.8 4,999,389 - PP_4407 0.17 +1.2 4,999,389 - PP_4407 0.17 -1.8 4,999,562 + PP_4407 0.30 -1.1 4,999,587 + PP_4407 0.32 -1.5 4,999,587 + +4.0 4,999,592 + PP_4407 0.32 +0.9 4,999,593 - PP_4407 0.32 +1.2 4,999,593 - PP_4407 0.32 -0.9 4,999,627 + PP_4407 0.35 +0.3 4,999,627 + PP_4407 0.35 +0.2 4,999,627 + PP_4407 0.35 -0.1 4,999,628 - PP_4407 0.35 -1.7 4,999,628 - PP_4407 0.35 -0.3 4,999,628 - PP_4407 0.35 -0.1 4,999,675 + PP_4407 0.38 -0.4 4,999,676 - PP_4407 0.38 +0.3 4,999,676 - PP_4407 0.38 -0.4 4,999,676 - PP_4407 0.38 -1.4 4,999,676 - PP_4407 0.38 +0.5 4,999,676 - PP_4407 0.38 +1.4 4,999,880 + PP_4407 0.53 -0.1 4,999,880 + PP_4407 0.53 -0.4 4,999,880 + PP_4407 0.53 -0.5 4,999,880 + PP_4407 0.53 -0.6 4,999,880 + PP_4407 0.53 -0.0 4,999,880 + PP_4407 0.53 -1.0 4,999,880 + PP_4407 0.53 +0.5 4,999,880 + PP_4407 0.53 -2.4 4,999,880 + PP_4407 0.53 +0.4 4,999,880 + PP_4407 0.53 -0.2 4,999,880 + PP_4407 0.53 +0.1 4,999,881 - PP_4407 0.53 -0.7 4,999,881 - PP_4407 0.53 -0.3 4,999,881 - PP_4407 0.53 +0.6 4,999,881 - PP_4407 0.53 -0.9 4,999,881 - PP_4407 0.53 -1.8 4,999,881 - PP_4407 0.53 -0.6 4,999,884 + PP_4407 0.53 -1.3 4,999,884 + PP_4407 0.53 -0.8 4,999,884 + PP_4407 0.53 -2.5 4,999,884 + PP_4407 0.53 -0.6 4,999,884 + PP_4407 0.53 -0.9 4,999,885 - +0.7 4,999,885 - PP_4407 0.53 +0.1 4,999,885 - PP_4407 0.53 -0.7 4,999,885 - PP_4407 0.53 -1.8 4,999,949 + PP_4407 0.58 -0.2 4,999,949 + PP_4407 0.58 +0.1 4,999,949 + PP_4407 0.58 -1.2 5,000,130 + PP_4407 0.71 -0.6 5,000,231 - PP_4407 0.78 -0.8 5,000,253 + PP_4407 0.80 -0.2 5,000,254 - PP_4407 0.80 -0.3 5,000,254 - PP_4407 0.80 -0.3 5,000,254 - PP_4407 0.80 +1.6 5,000,348 + PP_4407 0.87 -0.9 5,000,356 + PP_4407 0.87 +0.0 5,000,356 + PP_4407 0.87 -1.4 5,000,356 + PP_4407 0.87 -0.2 5,000,357 - PP_4407 0.87 -1.6 5,000,357 - PP_4407 0.87 -1.2 5,000,357 - PP_4407 0.87 -1.1 5,000,357 - PP_4407 0.87 +0.3 5,000,448 + +1.0 5,000,448 + +0.6 5,000,449 - +0.3 5,000,639 + -0.2 5,000,640 - -0.3 5,000,704 + -0.4 5,000,704 + -1.8 5,000,704 + +0.3 5,000,704 + -0.9 5,000,704 + -1.3 5,000,705 - -0.5 5,000,705 - +0.2 5,000,705 - -0.3 5,000,707 + -0.3 5,000,718 - -1.7 5,000,718 - +2.4 5,000,820 - -1.0 5,000,820 - +1.7 5,000,820 - +0.5 5,000,829 - -1.0 5,000,878 + -0.0 5,000,896 - +1.0 5,000,896 - -0.7 5,001,066 + +1.7 5,001,066 + -0.3 5,001,067 - -0.5 5,001,100 + +2.0 5,001,101 - +0.4 5,001,101 - -1.0 5,001,398 + -0.2 5,001,466 + +4.0 5,001,467 - +0.1 5,001,467 - +0.5 5,001,491 + -1.5 5,001,491 + +1.1 5,001,491 + -1.3
Or see this region's nucleotide sequence