Strain Fitness in Pseudomonas putida KT2440 around PP_4267

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
4,850,861	+		PP_4265	0.80	+0.2
4,850,861	+		PP_4265	0.80	+2.0
4,850,861	+		PP_4265	0.80	+1.0
4,850,862	-		PP_4265	0.81	+2.0
4,850,877	+		PP_4265	0.83	+1.2
4,851,015	+				-0.3
4,851,024	+				+4.0
4,851,024	+				-0.1
4,851,024	+				+0.5
4,851,025	-				-1.6
4,851,025	-				+0.7
4,851,143	+		PP_4266	0.15	-1.8
4,851,177	+		PP_4266	0.21	-0.2
4,851,178	-		PP_4266	0.21	+0.4
4,851,178	-		PP_4266	0.21	+0.7
4,851,349	-		PP_4266	0.53	+0.3
4,851,440	+		PP_4266	0.69	-1.9
4,851,440	+		PP_4266	0.69	-1.5
4,851,449	+		PP_4266	0.71	-0.2
4,851,450	-		PP_4266	0.71	+1.0
4,851,569	+				+2.7
4,851,569	+				-0.0
4,851,570	-				+1.8
4,851,570	-				-0.2
4,851,570	-				-0.6
4,851,570	-				-1.4
4,851,604	-				+0.7
4,851,604	-				+0.7
4,851,659	-				+1.0
4,851,867	-		PP_4267	0.23	-1.4
4,851,867	-		PP_4267	0.23	-0.7
4,851,867	-		PP_4267	0.23	+0.3
4,852,055	+		PP_4267	0.54	+0.8
4,852,055	+		PP_4267	0.54	-1.3
4,852,055	+		PP_4267	0.54	-1.0
4,852,055	+		PP_4267	0.54	+0.4
4,852,055	+		PP_4267	0.54	-1.0
4,852,055	+		PP_4267	0.54	-1.0
4,852,056	-		PP_4267	0.55	-1.2
4,852,056	-		PP_4267	0.55	-0.6
4,852,351	+				+1.0
4,852,351	+				-0.7
4,852,364	-				-0.8
4,852,365	+				+0.5
4,852,627	+				-0.5
4,852,628	-				+0.2
4,852,628	-				-1.2
4,852,752	+		PP_4268	0.38	+1.2
4,852,753	-		PP_4268	0.38	+0.0
4,852,967	-				+0.8
4,852,967	-				+2.4
4,852,969	-				+0.8
4,852,969	-				+0.6
4,852,969	-				+0.2
4,853,027	-				-1.0
4,853,039	+				-0.5
4,853,040	-				+0.6
4,853,053	-				+0.7
4,853,063	+				+4.0
4,853,063	+				+4.0
4,853,063	+				+3.0
4,853,064	-				+0.5
4,853,064	-				-0.4
4,853,064	-				+0.4
4,853,064	-				+0.2
4,853,064	-				+3.0
4,853,064	-				+2.0
4,853,066	+				+3.0
4,853,067	-				-0.3
4,853,067	-				+1.7
4,853,067	-				+1.0