Strain Fitness in Pseudomonas putida KT2440 around PP_4126

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4125 and PP_4126 are separated by 11 nucleotidesPP_4126 and PP_4127 are separated by 10 nucleotidesPP_4127 and PP_4128 are separated by 3 nucleotidesPP_4128 and PP_4129 overlap by 4 nucleotides PP_4125: PP_4125 - NADH-quinone oxidoreductase subunit H, at 4,663,848 to 4,664,855 _4125 PP_4126: PP_4126 - NADH-quinone oxidoreductase subunit I, at 4,664,867 to 4,665,415 _4126 PP_4127: PP_4127 - NADH-quinone oxidoreductase subunit J, at 4,665,426 to 4,665,926 _4127 PP_4128: PP_4128 - NADH-quinone oxidoreductase subunit K, at 4,665,930 to 4,666,238 _4128 PP_4129: PP_4129 - NADH:ubiquinone oxidoreductase, membrane subunit L, at 4,666,235 to 4,668,088 _4129 Position (kb) 4664 4665 4666Strain fitness (log2 ratio) -1 0 1 2 3 4at 4664.313 kb on + strandat 4664.314 kb on - strand, within PP_4125at 4664.574 kb on + strand, within PP_4125at 4664.574 kb on + strand, within PP_4125at 4664.574 kb on + strand, within PP_4125at 4664.575 kb on - strand, within PP_4125at 4664.826 kb on + strandat 4664.826 kb on + strandat 4664.826 kb on + strandat 4664.876 kb on + strandat 4664.876 kb on + strandat 4664.876 kb on + strandat 4664.877 kb on - strandat 4664.893 kb on + strandat 4664.894 kb on - strandat 4665.054 kb on + strand, within PP_4126at 4665.266 kb on + strand, within PP_4126at 4665.266 kb on + strand, within PP_4126at 4665.323 kb on + strand, within PP_4126at 4665.323 kb on + strandat 4665.324 kb on - strand, within PP_4126at 4665.324 kb on - strand, within PP_4126at 4665.441 kb on + strandat 4665.441 kb on + strandat 4665.484 kb on + strand, within PP_4127at 4665.750 kb on + strand, within PP_4127at 4665.750 kb on + strand, within PP_4127at 4665.751 kb on - strand, within PP_4127at 4665.924 kb on + strandat 4666.373 kb on + strandat 4666.373 kb on + strandat 4666.374 kb on - strandat 4666.374 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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4,664,313 + +4.0
4,664,314 - PP_4125 0.46 +3.0
4,664,574 + PP_4125 0.72 +3.0
4,664,574 + PP_4125 0.72 +3.0
4,664,574 + PP_4125 0.72 +4.0
4,664,575 - PP_4125 0.72 -0.2
4,664,826 + -0.8
4,664,826 + +0.5
4,664,826 + -0.3
4,664,876 + +3.0
4,664,876 + +4.0
4,664,876 + +1.0
4,664,877 - +4.0
4,664,893 + +4.0
4,664,894 - +2.0
4,665,054 + PP_4126 0.34 +4.0
4,665,266 + PP_4126 0.73 +4.0
4,665,266 + PP_4126 0.73 +3.0
4,665,323 + PP_4126 0.83 +4.0
4,665,323 + +4.0
4,665,324 - PP_4126 0.83 +1.4
4,665,324 - PP_4126 0.83 +4.0
4,665,441 + +3.0
4,665,441 + +0.8
4,665,484 + PP_4127 0.12 -0.5
4,665,750 + PP_4127 0.65 +0.7
4,665,750 + PP_4127 0.65 +2.0
4,665,751 - PP_4127 0.65 +2.0
4,665,924 + +3.0
4,666,373 + +3.0
4,666,373 + +0.4
4,666,374 - +4.0
4,666,374 - +3.0

Or see this region's nucleotide sequence