Strain Fitness in Pseudomonas putida KT2440 around PP_4070

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
4,594,605	+		PP_4068	0.87	-0.6
4,594,605	+		PP_4068	0.87	-1.5
4,594,606	-		PP_4068	0.87	-1.7
4,594,606	-		PP_4068	0.87	-1.6
4,594,606	-		PP_4068	0.87	+4.0
4,594,606	-		PP_4068	0.87	-1.0
4,594,643	+				-1.5
4,594,643	+				+0.5
4,594,644	-				+2.4
4,594,644	-				-0.6
4,594,644	-				-2.1
4,594,649	+				+0.3
4,594,649	+				+2.4
4,594,650	-				-0.2
4,594,650	-				-2.4
4,594,668	-				-0.2
4,594,778	+				-0.1
4,594,792	+				+0.2
4,594,851	+		PP_4069	0.22	-0.8
4,594,851	+		PP_4069	0.22	-1.6
4,594,851	+		PP_4069	0.22	-1.1
4,594,852	-		PP_4069	0.22	-0.8
4,595,093	-				+1.4
4,595,093	-				+0.2
4,595,093	-				-0.9
4,595,094	+				-1.4
4,595,094	+				-2.5
4,595,095	-				+0.1
4,595,095	-				-0.8
4,595,095	-				+0.5
4,595,115	+				+0.2
4,595,115	+				-0.5
4,595,115	+				-1.6
4,595,116	-				-1.9
4,595,192	+				+0.3
4,595,293	+				-1.1
4,595,309	+				+1.2
4,595,310	-				-2.5
4,595,310	-				-0.5
4,595,393	-				+0.5
4,595,513	+				-0.8
4,595,513	+				+1.6
4,595,513	+				-0.5
4,595,514	-				-0.6
4,595,514	-				-1.2
4,595,514	-				-0.1
4,595,514	-				-0.9
4,595,516	-				+0.4
4,595,516	-				-0.6
4,595,516	-				-0.1
4,595,516	-				+1.3
4,595,642	+		PP_4070	0.21	-1.1
4,595,642	+		PP_4070	0.21	-0.4
4,595,642	+		PP_4070	0.21	-0.1
4,595,643	-		PP_4070	0.21	-0.0
4,595,643	-		PP_4070	0.21	-1.4
4,595,643	-		PP_4070	0.21	-1.7
4,595,794	+		PP_4070	0.51	-0.0
4,595,794	+				+1.6
4,595,794	+		PP_4070	0.51	-2.1
4,595,795	-		PP_4070	0.51	+1.2
4,595,795	-		PP_4070	0.51	-0.8
4,595,836	-		PP_4070	0.59	-2.0
4,595,892	+		PP_4070	0.70	-1.2
4,596,070	-				-0.6
4,596,070	-				+1.6
4,596,070	-				+0.7
4,596,070	-				-0.1
4,596,070	-				-0.1
4,596,124	-				+0.3
4,596,133	-				-0.0
4,596,184	-				-1.7
4,596,184	-				-0.3
4,596,280	+				-0.0
4,596,284	-				-0.4
4,596,284	-				+2.0
4,596,423	+				+0.1
4,596,423	+				-0.1
4,596,423	+				+0.4
4,596,439	+				-0.2
4,596,439	+				-1.9
4,596,440	-				-0.4
4,596,440	-				+0.1
4,596,440	-				+1.4
4,596,509	-				+0.3
4,596,537	+				-1.7
4,596,538	-				-0.7
4,596,607	+		PP_4071	0.12	+0.3
4,596,765	+		PP_4071	0.16	+0.3
4,596,766	-		PP_4071	0.16	-0.4
4,596,766	-		PP_4071	0.16	-0.4
4,596,766	-		PP_4071	0.16	+1.2
4,596,848	+		PP_4071	0.18	-0.9
4,596,849	-				+2.4
4,596,858	+		PP_4071	0.19	-0.8
4,596,973	+		PP_4071	0.22	-1.4
4,596,973	+		PP_4071	0.22	+0.3
4,596,973	+		PP_4071	0.22	+0.0
4,596,974	-		PP_4071	0.22	-0.0