Strain Fitness in Pseudomonas putida KT2440 around PP_4050

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4049 and PP_4050 are separated by 897 nucleotidesPP_4050 and PP_4051 are separated by 14 nucleotides PP_4049: PP_4049 - type 6 secretion system protein, at 4,561,545 to 4,563,860 _4049 PP_4050: PP_4050 - glycogen synthase, at 4,564,758 to 4,566,317 _4050 PP_4051: PP_4051 - malto-oligosyltrehalose trehalohydrolase, at 4,566,332 to 4,568,074 _4051 Position (kb) 4564 4565 4566 4567Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 4563.931 kb on + strandat 4563.972 kb on - strandat 4564.079 kb on + strandat 4564.139 kb on + strandat 4564.140 kb on - strandat 4564.214 kb on - strandat 4564.243 kb on + strandat 4564.245 kb on + strandat 4564.245 kb on + strandat 4564.246 kb on - strandat 4564.246 kb on - strandat 4564.298 kb on + strandat 4564.299 kb on - strandat 4564.299 kb on - strandat 4564.369 kb on + strandat 4564.369 kb on + strandat 4564.369 kb on + strandat 4564.370 kb on - strandat 4564.390 kb on - strandat 4564.390 kb on - strandat 4564.407 kb on + strandat 4564.463 kb on + strandat 4564.549 kb on + strandat 4564.549 kb on + strandat 4564.549 kb on + strandat 4564.549 kb on + strandat 4564.549 kb on + strandat 4564.550 kb on - strandat 4564.550 kb on - strandat 4564.550 kb on - strandat 4564.550 kb on - strandat 4564.550 kb on - strandat 4564.553 kb on + strandat 4564.553 kb on + strandat 4564.741 kb on + strandat 4564.741 kb on + strandat 4564.742 kb on - strandat 4564.742 kb on - strandat 4564.742 kb on - strandat 4564.757 kb on + strandat 4564.757 kb on + strandat 4564.757 kb on + strandat 4564.758 kb on - strandat 4564.758 kb on - strandat 4564.758 kb on - strandat 4565.039 kb on + strand, within PP_4050at 4565.039 kb on + strand, within PP_4050at 4565.040 kb on - strand, within PP_4050at 4565.040 kb on - strand, within PP_4050at 4565.040 kb on - strand, within PP_4050at 4565.040 kb on - strand, within PP_4050at 4565.040 kb on - strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.041 kb on + strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.042 kb on - strand, within PP_4050at 4565.045 kb on + strand, within PP_4050at 4565.045 kb on + strand, within PP_4050at 4565.045 kb on + strand, within PP_4050at 4565.046 kb on - strand, within PP_4050at 4565.046 kb on - strand, within PP_4050at 4565.046 kb on - strand, within PP_4050at 4565.046 kb on - strand, within PP_4050at 4565.046 kb on - strand, within PP_4050at 4565.046 kb on - strand, within PP_4050at 4565.127 kb on + strand, within PP_4050at 4565.127 kb on + strand, within PP_4050at 4565.127 kb on + strand, within PP_4050at 4565.127 kb on + strand, within PP_4050at 4565.127 kb on + strandat 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.128 kb on - strand, within PP_4050at 4565.172 kb on + strand, within PP_4050at 4565.172 kb on + strand, within PP_4050at 4565.173 kb on - strand, within PP_4050at 4565.173 kb on - strand, within PP_4050at 4565.173 kb on - strand, within PP_4050at 4565.328 kb on + strand, within PP_4050at 4565.352 kb on - strand, within PP_4050at 4565.385 kb on + strand, within PP_4050at 4565.397 kb on + strand, within PP_4050at 4565.397 kb on + strand, within PP_4050at 4565.535 kb on + strandat 4565.535 kb on + strand, within PP_4050at 4565.536 kb on - strand, within PP_4050at 4565.536 kb on - strand, within PP_4050at 4565.536 kb on - strand, within PP_4050at 4565.714 kb on + strand, within PP_4050at 4565.832 kb on + strand, within PP_4050at 4565.832 kb on + strand, within PP_4050at 4565.833 kb on - strand, within PP_4050at 4565.833 kb on - strand, within PP_4050at 4566.039 kb on + strand, within PP_4050at 4566.040 kb on - strand, within PP_4050at 4566.074 kb on + strand, within PP_4050at 4566.075 kb on - strand, within PP_4050at 4566.075 kb on - strand, within PP_4050at 4566.075 kb on - strand, within PP_4050at 4566.075 kb on - strand, within PP_4050at 4566.075 kb on - strand, within PP_4050at 4566.151 kb on - strand, within PP_4050at 4566.151 kb on - strand, within PP_4050at 4566.177 kb on + strandat 4566.178 kb on - strandat 4566.178 kb on - strandat 4566.184 kb on - strandat 4566.264 kb on + strandat 4566.265 kb on - strandat 4566.277 kb on - strandat 4566.313 kb on - strandat 4566.503 kb on + strandat 4566.509 kb on + strand, within PP_4051at 4566.588 kb on + strand, within PP_4051at 4566.593 kb on + strand, within PP_4051at 4566.788 kb on + strand, within PP_4051at 4566.788 kb on + strand, within PP_4051at 4566.798 kb on + strand, within PP_4051at 4566.806 kb on + strand, within PP_4051at 4566.824 kb on + strand, within PP_4051at 4566.902 kb on + strand, within PP_4051at 4566.929 kb on + strand, within PP_4051at 4567.023 kb on - strand, within PP_4051at 4567.221 kb on - strand, within PP_4051at 4567.289 kb on + strand, within PP_4051at 4567.289 kb on + strand, within PP_4051at 4567.290 kb on - strand, within PP_4051at 4567.292 kb on + strand, within PP_4051at 4567.292 kb on + strand, within PP_4051at 4567.292 kb on + strand, within PP_4051at 4567.293 kb on - strand, within PP_4051at 4567.293 kb on - strand, within PP_4051

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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4,563,931 + -0.6
4,563,972 - -0.1
4,564,079 + +1.6
4,564,139 + +1.0
4,564,140 - +0.5
4,564,214 - +0.5
4,564,243 + -0.9
4,564,245 + -3.0
4,564,245 + -0.4
4,564,246 - -1.4
4,564,246 - -0.4
4,564,298 + +1.1
4,564,299 - -0.4
4,564,299 - -1.1
4,564,369 + +1.2
4,564,369 + +0.9
4,564,369 + +0.1
4,564,370 - -0.2
4,564,390 - +0.3
4,564,390 - +0.1
4,564,407 + +4.0
4,564,463 + +4.0
4,564,549 + +4.0
4,564,549 + +2.4
4,564,549 + +4.0
4,564,549 + +5.0
4,564,549 + +3.0
4,564,550 - +0.9
4,564,550 - +0.8
4,564,550 - +1.2
4,564,550 - -0.3
4,564,550 - -1.1
4,564,553 + +1.0
4,564,553 + +2.0
4,564,741 + -0.3
4,564,741 + +2.0
4,564,742 - +1.6
4,564,742 - -0.0
4,564,742 - +0.1
4,564,757 + +1.2
4,564,757 + +0.4
4,564,757 + +0.4
4,564,758 - +0.4
4,564,758 - +0.9
4,564,758 - +0.9
4,565,039 + PP_4050 0.18 +0.6
4,565,039 + PP_4050 0.18 -0.2
4,565,040 - PP_4050 0.18 +0.4
4,565,040 - PP_4050 0.18 -0.4
4,565,040 - PP_4050 0.18 +0.3
4,565,040 - PP_4050 0.18 -0.1
4,565,040 - PP_4050 0.18 +0.8
4,565,041 + PP_4050 0.18 -0.7
4,565,041 + PP_4050 0.18 -0.1
4,565,041 + PP_4050 0.18 -0.1
4,565,041 + PP_4050 0.18 +0.4
4,565,041 + PP_4050 0.18 +1.9
4,565,041 + PP_4050 0.18 -0.2
4,565,041 + PP_4050 0.18 +0.7
4,565,041 + PP_4050 0.18 -0.9
4,565,041 + PP_4050 0.18 +0.2
4,565,041 + PP_4050 0.18 +0.3
4,565,041 + PP_4050 0.18 +0.0
4,565,042 - PP_4050 0.18 -0.1
4,565,042 - PP_4050 0.18 +0.1
4,565,042 - PP_4050 0.18 +0.2
4,565,042 - PP_4050 0.18 -0.1
4,565,042 - PP_4050 0.18 +0.9
4,565,042 - PP_4050 0.18 -0.5
4,565,042 - PP_4050 0.18 +0.0
4,565,045 + PP_4050 0.18 +0.6
4,565,045 + PP_4050 0.18 +0.9
4,565,045 + PP_4050 0.18 -0.4
4,565,046 - PP_4050 0.18 +0.6
4,565,046 - PP_4050 0.18 +1.2
4,565,046 - PP_4050 0.18 +1.1
4,565,046 - PP_4050 0.18 -0.3
4,565,046 - PP_4050 0.18 -0.1
4,565,046 - PP_4050 0.18 +1.3
4,565,127 + PP_4050 0.24 +1.2
4,565,127 + PP_4050 0.24 +0.4
4,565,127 + PP_4050 0.24 +0.6
4,565,127 + PP_4050 0.24 +1.2
4,565,127 + +2.4
4,565,128 - PP_4050 0.24 -0.6
4,565,128 - PP_4050 0.24 -0.6
4,565,128 - PP_4050 0.24 -0.1
4,565,128 - PP_4050 0.24 -0.5
4,565,128 - PP_4050 0.24 -0.9
4,565,128 - PP_4050 0.24 -0.0
4,565,128 - PP_4050 0.24 -0.0
4,565,128 - PP_4050 0.24 -0.6
4,565,172 + PP_4050 0.27 +0.6
4,565,172 + PP_4050 0.27 +1.0
4,565,173 - PP_4050 0.27 +1.0
4,565,173 - PP_4050 0.27 +0.6
4,565,173 - PP_4050 0.27 +0.5
4,565,328 + PP_4050 0.37 -0.4
4,565,352 - PP_4050 0.38 -0.8
4,565,385 + PP_4050 0.40 +0.5
4,565,397 + PP_4050 0.41 +0.1
4,565,397 + PP_4050 0.41 +1.3
4,565,535 + +2.4
4,565,535 + PP_4050 0.50 +0.2
4,565,536 - PP_4050 0.50 +0.5
4,565,536 - PP_4050 0.50 +0.0
4,565,536 - PP_4050 0.50 +0.7
4,565,714 + PP_4050 0.61 -1.0
4,565,832 + PP_4050 0.69 +0.2
4,565,832 + PP_4050 0.69 +0.2
4,565,833 - PP_4050 0.69 +0.0
4,565,833 - PP_4050 0.69 +0.5
4,566,039 + PP_4050 0.82 +0.4
4,566,040 - PP_4050 0.82 +0.9
4,566,074 + PP_4050 0.84 -0.1
4,566,075 - PP_4050 0.84 +1.1
4,566,075 - PP_4050 0.84 +1.1
4,566,075 - PP_4050 0.84 -0.5
4,566,075 - PP_4050 0.84 +0.2
4,566,075 - PP_4050 0.84 +0.6
4,566,151 - PP_4050 0.89 -1.4
4,566,151 - PP_4050 0.89 +1.2
4,566,177 + -0.4
4,566,178 - -0.5
4,566,178 - +0.4
4,566,184 - -0.9
4,566,264 + +0.0
4,566,265 - +0.4
4,566,277 - +0.9
4,566,313 - +0.6
4,566,503 + -1.2
4,566,509 + PP_4051 0.10 +1.6
4,566,588 + PP_4051 0.15 +1.8
4,566,593 + PP_4051 0.15 +0.2
4,566,788 + PP_4051 0.26 +0.1
4,566,788 + PP_4051 0.26 -0.8
4,566,798 + PP_4051 0.27 +0.6
4,566,806 + PP_4051 0.27 -0.3
4,566,824 + PP_4051 0.28 +0.4
4,566,902 + PP_4051 0.33 -0.1
4,566,929 + PP_4051 0.34 +1.3
4,567,023 - PP_4051 0.40 -0.9
4,567,221 - PP_4051 0.51 -0.5
4,567,289 + PP_4051 0.55 -1.3
4,567,289 + PP_4051 0.55 +0.1
4,567,290 - PP_4051 0.55 +4.0
4,567,292 + PP_4051 0.55 +0.9
4,567,292 + PP_4051 0.55 +1.4
4,567,292 + PP_4051 0.55 +0.5
4,567,293 - PP_4051 0.55 +0.6
4,567,293 - PP_4051 0.55 -0.3

Or see this region's nucleotide sequence