Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4012 and PP_4013 are separated by 192 nucleotides PP_4013 and PP_4014 are separated by 59 nucleotides PP_4014 and PP_4015 overlap by 4 nucleotides PP_4015 and PP_4016 are separated by 137 nucleotides
PP_4012: PP_4012 - isocitrate dehydrogenase, at 4,522,152 to 4,524,377
_4012
PP_4013: PP_4013 - MutT/nudix family protein, at 4,524,570 to 4,525,010
_4013
PP_4014: PP_4014 - tRNA-specific 2-thiouridylase MnmA, at 4,525,070 to 4,526,194
_4014
PP_4015: PP_4015 - HflD-like high frequency lysogenization protein, at 4,526,191 to 4,526,817
_4015
PP_4016: PP_4016 - 5'-phosphoribosyl-4-(N-succinocarboxamide)-5- aminoimidazole lyase (adenylosuccinate lyase), at 4,526,955 to 4,528,325
_4016
Position (kb) 4525
4526
4527 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4524.375 kb on + strand at 4524.376 kb on - strand at 4524.391 kb on + strand at 4524.391 kb on + strand at 4524.444 kb on + strand at 4524.444 kb on + strand at 4524.471 kb on - strand at 4524.471 kb on - strand at 4524.483 kb on - strand at 4524.536 kb on + strand at 4524.537 kb on - strand at 4524.777 kb on + strand, within PP_4013 at 4524.777 kb on + strand, within PP_4013 at 4524.778 kb on - strand, within PP_4013 at 4524.778 kb on - strand, within PP_4013 at 4524.778 kb on - strand, within PP_4013 at 4524.783 kb on + strand, within PP_4013 at 4524.784 kb on - strand, within PP_4013 at 4524.875 kb on + strand, within PP_4013 at 4524.967 kb on - strand at 4525.055 kb on + strand at 4526.596 kb on + strand, within PP_4015 at 4526.596 kb on + strand, within PP_4015 at 4526.596 kb on + strand, within PP_4015 at 4526.596 kb on + strand at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.597 kb on - strand, within PP_4015 at 4526.910 kb on + strand at 4526.910 kb on + strand at 4526.910 kb on + strand at 4526.915 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,524,375 + -0.1 4,524,376 - +0.1 4,524,391 + +1.4 4,524,391 + -0.4 4,524,444 + -1.2 4,524,444 + +0.5 4,524,471 - -0.2 4,524,471 - +2.0 4,524,483 - +0.6 4,524,536 + +0.6 4,524,537 - -0.5 4,524,777 + PP_4013 0.47 -0.6 4,524,777 + PP_4013 0.47 -0.0 4,524,778 - PP_4013 0.47 -0.2 4,524,778 - PP_4013 0.47 -1.8 4,524,778 - PP_4013 0.47 +0.8 4,524,783 + PP_4013 0.48 -0.8 4,524,784 - PP_4013 0.49 +0.1 4,524,875 + PP_4013 0.69 -0.8 4,524,967 - -1.9 4,525,055 + +3.0 4,526,596 + PP_4015 0.65 -0.3 4,526,596 + PP_4015 0.65 +0.2 4,526,596 + PP_4015 0.65 -0.4 4,526,596 + -0.8 4,526,597 - PP_4015 0.65 -1.5 4,526,597 - PP_4015 0.65 -1.4 4,526,597 - PP_4015 0.65 -0.1 4,526,597 - PP_4015 0.65 -2.4 4,526,597 - PP_4015 0.65 +0.0 4,526,910 + +0.3 4,526,910 + +1.1 4,526,910 + -0.5 4,526,915 + +3.0
Or see this region's nucleotide sequence