Strain Fitness in Pseudomonas putida KT2440 around PP_4013

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_4012 and PP_4013 are separated by 192 nucleotidesPP_4013 and PP_4014 are separated by 59 nucleotides PP_4012: PP_4012 - isocitrate dehydrogenase, at 4,522,152 to 4,524,377 _4012 PP_4013: PP_4013 - MutT/nudix family protein, at 4,524,570 to 4,525,010 _4013 PP_4014: PP_4014 - tRNA-specific 2-thiouridylase MnmA, at 4,525,070 to 4,526,194 _4014 Position (kb) 4524 4525 4526Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4524.375 kb on + strandat 4524.376 kb on - strandat 4524.391 kb on + strandat 4524.391 kb on + strandat 4524.444 kb on + strandat 4524.444 kb on + strandat 4524.471 kb on - strandat 4524.471 kb on - strandat 4524.483 kb on - strandat 4524.536 kb on + strandat 4524.537 kb on - strandat 4524.777 kb on + strand, within PP_4013at 4524.777 kb on + strand, within PP_4013at 4524.778 kb on - strand, within PP_4013at 4524.778 kb on - strand, within PP_4013at 4524.778 kb on - strand, within PP_4013at 4524.783 kb on + strand, within PP_4013at 4524.784 kb on - strand, within PP_4013at 4524.875 kb on + strand, within PP_4013at 4524.967 kb on - strandat 4525.055 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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4,524,375 + -0.1
4,524,376 - +0.1
4,524,391 + +1.4
4,524,391 + -0.4
4,524,444 + -1.2
4,524,444 + +0.5
4,524,471 - -0.2
4,524,471 - +2.0
4,524,483 - +0.6
4,524,536 + +0.6
4,524,537 - -0.5
4,524,777 + PP_4013 0.47 -0.6
4,524,777 + PP_4013 0.47 -0.0
4,524,778 - PP_4013 0.47 -0.2
4,524,778 - PP_4013 0.47 -1.8
4,524,778 - PP_4013 0.47 +0.8
4,524,783 + PP_4013 0.48 -0.8
4,524,784 - PP_4013 0.49 +0.1
4,524,875 + PP_4013 0.69 -0.8
4,524,967 - -1.9
4,525,055 + +3.0

Or see this region's nucleotide sequence