Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_3805 and PP_3806 are separated by 26 nucleotides PP_3806 and PP_3807 are separated by 56 nucleotides PP_3807 and PP_3808 overlap by 4 nucleotides
PP_3805: PP_3805 - lipoprotein required for swarming, at 4,333,144 to 4,334,196
_3805
PP_3806: PP_3806 - Hydrolase, isochorismatase family, at 4,334,223 to 4,334,774
_3806
PP_3807: PP_3807 - Thioesterase, at 4,334,831 to 4,335,550
_3807
PP_3808: PP_3808 - Antibiotic synthesis protein MbtH, at 4,335,547 to 4,335,768
_3808
Position (kb)
4334
4335 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4333.372 kb on - strand, within PP_3805 at 4333.372 kb on - strand, within PP_3805 at 4333.554 kb on - strand, within PP_3805 at 4333.568 kb on + strand, within PP_3805 at 4333.568 kb on + strand, within PP_3805 at 4333.568 kb on + strand, within PP_3805 at 4333.569 kb on - strand, within PP_3805 at 4333.607 kb on + strand, within PP_3805 at 4333.607 kb on + strand, within PP_3805 at 4333.607 kb on + strand, within PP_3805 at 4333.607 kb on + strand, within PP_3805 at 4333.608 kb on - strand, within PP_3805 at 4333.608 kb on - strand, within PP_3805 at 4333.608 kb on - strand, within PP_3805 at 4333.661 kb on + strand, within PP_3805 at 4333.787 kb on + strand, within PP_3805 at 4333.787 kb on + strand, within PP_3805 at 4333.838 kb on + strand, within PP_3805 at 4333.838 kb on + strand, within PP_3805 at 4333.896 kb on + strand, within PP_3805 at 4333.896 kb on + strand, within PP_3805 at 4333.897 kb on - strand, within PP_3805 at 4333.897 kb on - strand, within PP_3805 at 4334.102 kb on - strand at 4334.245 kb on + strand at 4334.322 kb on + strand, within PP_3806 at 4334.322 kb on + strand, within PP_3806 at 4334.322 kb on + strand, within PP_3806 at 4334.575 kb on - strand, within PP_3806 at 4334.801 kb on + strand at 4335.051 kb on + strand, within PP_3807 at 4335.051 kb on + strand, within PP_3807 at 4335.051 kb on + strand, within PP_3807 at 4335.052 kb on - strand, within PP_3807 at 4335.052 kb on - strand, within PP_3807 at 4335.207 kb on + strand, within PP_3807 at 4335.207 kb on + strand, within PP_3807 at 4335.208 kb on - strand, within PP_3807 at 4335.208 kb on - strand, within PP_3807 at 4335.223 kb on + strand, within PP_3807 at 4335.339 kb on + strand, within PP_3807 at 4335.339 kb on + strand, within PP_3807 at 4335.339 kb on + strand, within PP_3807 at 4335.339 kb on + strand, within PP_3807 at 4335.339 kb on + strand, within PP_3807 at 4335.340 kb on - strand, within PP_3807 at 4335.447 kb on - strand, within PP_3807 at 4335.455 kb on + strand, within PP_3807 at 4335.456 kb on - strand, within PP_3807 at 4335.496 kb on - strand at 4335.520 kb on - strand at 4335.620 kb on + strand, within PP_3808 at 4335.704 kb on + strand, within PP_3808
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 4,333,372 - PP_3805 0.22 +0.0 4,333,372 - PP_3805 0.22 -2.0 4,333,554 - PP_3805 0.39 -0.2 4,333,568 + PP_3805 0.40 -0.8 4,333,568 + PP_3805 0.40 +0.8 4,333,568 + PP_3805 0.40 +0.8 4,333,569 - PP_3805 0.40 -0.2 4,333,607 + PP_3805 0.44 +0.2 4,333,607 + PP_3805 0.44 -0.3 4,333,607 + PP_3805 0.44 +0.6 4,333,607 + PP_3805 0.44 -0.4 4,333,608 - PP_3805 0.44 -1.1 4,333,608 - PP_3805 0.44 -0.5 4,333,608 - PP_3805 0.44 +2.0 4,333,661 + PP_3805 0.49 -1.7 4,333,787 + PP_3805 0.61 -0.7 4,333,787 + PP_3805 0.61 -0.6 4,333,838 + PP_3805 0.66 -0.9 4,333,838 + PP_3805 0.66 +0.1 4,333,896 + PP_3805 0.71 +0.9 4,333,896 + PP_3805 0.71 -0.4 4,333,897 - PP_3805 0.72 -0.3 4,333,897 - PP_3805 0.72 -0.1 4,334,102 - -0.0 4,334,245 + +0.0 4,334,322 + PP_3806 0.18 +0.4 4,334,322 + PP_3806 0.18 -0.7 4,334,322 + PP_3806 0.18 -0.9 4,334,575 - PP_3806 0.64 -0.6 4,334,801 + -0.6 4,335,051 + PP_3807 0.31 +1.2 4,335,051 + PP_3807 0.31 +1.0 4,335,051 + PP_3807 0.31 +1.8 4,335,052 - PP_3807 0.31 +2.0 4,335,052 - PP_3807 0.31 +1.0 4,335,207 + PP_3807 0.52 -1.0 4,335,207 + PP_3807 0.52 -0.1 4,335,208 - PP_3807 0.52 +0.4 4,335,208 - PP_3807 0.52 -0.1 4,335,223 + PP_3807 0.54 +1.4 4,335,339 + PP_3807 0.71 +0.2 4,335,339 + PP_3807 0.71 +0.6 4,335,339 + PP_3807 0.71 -0.8 4,335,339 + PP_3807 0.71 +1.7 4,335,339 + PP_3807 0.71 -0.1 4,335,340 - PP_3807 0.71 +0.5 4,335,447 - PP_3807 0.86 -0.4 4,335,455 + PP_3807 0.87 +0.5 4,335,456 - PP_3807 0.87 +0.0 4,335,496 - -1.3 4,335,520 - -0.5 4,335,620 + PP_3808 0.33 +0.5 4,335,704 + PP_3808 0.71 +0.1
Or see this region's nucleotide sequence