Strain Fitness in Pseudomonas putida KT2440 around PP_3543

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
4,013,682	-		PP_3541	0.16	-0.2
4,013,765	+		PP_3541	0.32	-0.5
4,013,815	+		PP_3541	0.42	+0.3
4,014,142	+				-0.4
4,014,199	-				+1.0
4,014,199	-				-1.3
4,014,261	-				-0.3
4,014,519	+				+0.6
4,014,519	+				-0.4
4,014,562	+				-1.1
4,014,605	+				+0.3
4,014,606	-				+0.1
4,014,606	-				-0.9
4,014,654	-				-0.6
4,014,654	-				+0.4
4,014,666	-				-1.0
4,014,725	+				-1.3
4,014,748	-				+0.1
4,014,752	+				-0.6
4,014,752	+				-0.8
4,014,958	-		PP_3543	0.22	-0.8
4,014,981	+		PP_3543	0.24	-1.1
4,015,263	+		PP_3543	0.44	+3.9
4,015,263	+		PP_3543	0.44	-1.0
4,015,264	-		PP_3543	0.44	-0.5
4,015,264	-		PP_3543	0.44	-0.1
4,015,264	-		PP_3543	0.44	-0.6
4,015,264	-		PP_3543	0.44	+0.3
4,015,265	+		PP_3543	0.44	+0.0
4,015,265	+		PP_3543	0.44	-0.4
4,015,266	-		PP_3543	0.44	-0.4
4,015,266	-		PP_3543	0.44	-0.2
4,015,266	-		PP_3543	0.44	-1.1
4,015,591	-		PP_3543	0.67	-0.8
4,015,591	-		PP_3543	0.67	-0.8
4,015,786	-		PP_3543	0.81	-1.3
4,015,786	-		PP_3543	0.81	-0.6
4,015,817	+		PP_3543	0.83	-0.8
4,015,817	+				+1.3
4,015,827	+		PP_3543	0.84	-2.6
4,015,827	+		PP_3543	0.84	-0.8
4,016,054	+				+0.6
4,016,054	+				-0.3
4,016,055	-				-1.9
4,016,055	-				-0.1
4,016,055	-				-0.9
4,016,190	-				-0.3
4,016,237	+				+0.0
4,016,237	+				+0.4
4,016,240	+		PP_3544	0.10	-0.8
4,016,241	-		PP_3544	0.10	+1.1
4,016,241	-		PP_3544	0.10	-0.8
4,016,241	-		PP_3544	0.10	-0.1
4,016,241	-		PP_3544	0.10	+1.0
4,016,241	-		PP_3544	0.10	+0.4
4,016,382	+		PP_3544	0.20	-0.9
4,016,576	+		PP_3544	0.34	+0.0
4,016,577	-				+0.5
4,016,708	+		PP_3544	0.43	+0.3
4,016,708	+		PP_3544	0.43	+0.1
4,016,708	+		PP_3544	0.43	-0.6
4,016,708	+		PP_3544	0.43	+0.7
4,016,708	+		PP_3544	0.43	-0.6
4,016,708	+		PP_3544	0.43	+0.6
4,016,709	-		PP_3544	0.43	-0.6
4,016,709	-		PP_3544	0.43	+0.3
4,016,709	-		PP_3544	0.43	-2.6
4,016,709	-		PP_3544	0.43	-1.5
4,016,709	-		PP_3544	0.43	-0.4
4,016,709	-		PP_3544	0.43	-0.4
4,016,709	-		PP_3544	0.43	+0.2
4,016,709	-		PP_3544	0.43	+0.2
4,016,961	-		PP_3544	0.61	-0.7
4,016,961	-		PP_3544	0.61	-0.4
4,016,961	-		PP_3544	0.61	-0.1
4,016,963	+		PP_3544	0.61	+0.5
4,016,963	+		PP_3544	0.61	+0.4