Strain Fitness in Pseudomonas putida KT2440 around PP_3197

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,625,783	+		PP_3196	0.23	-1.3
3,625,856	+		PP_3196	0.31	-0.9
3,625,910	-		PP_3196	0.37	-0.0
3,625,934	+		PP_3196	0.39	-0.2
3,625,934	+		PP_3196	0.39	-0.2
3,625,934	+		PP_3196	0.39	-0.6
3,625,934	+		PP_3196	0.39	-0.0
3,625,935	-		PP_3196	0.40	-0.5
3,626,039	+		PP_3196	0.51	-0.7
3,626,039	+		PP_3196	0.51	-0.3
3,626,040	-		PP_3196	0.51	-0.1
3,626,124	+		PP_3196	0.60	+0.4
3,626,125	-		PP_3196	0.60	-2.1
3,626,170	+		PP_3196	0.65	+1.3
3,626,186	+		PP_3196	0.66	-0.5
3,626,394	+		PP_3196	0.88	+0.6
3,626,508	+				+0.5
3,626,508	+				+0.4
3,626,508	+				+0.5
3,626,508	+				-0.6
3,626,509	-				+0.6
3,626,509	-				-0.1
3,626,509	-				-0.3
3,626,524	+				-0.8
3,626,525	-				+1.7
3,626,529	-				-0.6
3,626,561	+				+0.4
3,626,561	+				-0.8
3,626,585	+				+0.2
3,626,625	-				+0.8
3,626,705	-				+1.4
3,626,755	-				-0.0
3,626,775	-				+1.2
3,626,784	+				+0.5
3,626,785	-				-0.1
3,626,785	-				+1.6
3,626,785	-				-0.4
3,626,855	-				-0.5
3,626,925	+		PP_3197	0.15	-0.2
3,627,163	-		PP_3197	0.41	-0.6
3,627,163	-		PP_3197	0.41	-0.6
3,627,259	+		PP_3197	0.51	+0.7
3,627,259	+		PP_3197	0.51	+1.3
3,627,441	+		PP_3197	0.70	-1.0
3,627,839	+		PP_3198	0.31	+0.5
3,627,934	+		PP_3198	0.59	-0.3
3,627,934	+				+0.5
3,627,935	-		PP_3198	0.60	-0.2
3,628,043	+				+0.4
3,628,044	-				+0.5
3,628,146	+				-0.5
3,628,216	+				+0.2
3,628,216	+				+3.2
3,628,217	-				+0.0
3,628,335	-		PP_3199	0.11	-0.1
3,628,403	+		PP_3199	0.17	+0.0
3,628,442	+		PP_3199	0.21	+0.2
3,628,442	+		PP_3199	0.21	-1.0
3,628,443	-		PP_3199	0.21	+1.4
3,628,443	-		PP_3199	0.21	-1.6
3,628,443	-		PP_3199	0.21	+0.1
3,628,665	-		PP_3199	0.40	-1.5