Strain Fitness in Pseudomonas putida KT2440 around PP_3194

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,621,760	+		PP_3192	0.54	+0.9
3,621,855	-		PP_3192	0.70	+0.8
3,621,923	+		PP_3192	0.81	-1.8
3,621,933	-		PP_3192	0.83	+1.5
3,621,955	-		PP_3192	0.86	-0.3
3,621,985	+				+0.4
3,622,043	-				-1.4
3,622,069	-				+0.8
3,622,069	-				-0.4
3,622,087	-				-0.1
3,622,157	-				+0.6
3,622,626	-		PP_3193	0.79	+0.5
3,622,752	+				-1.0
3,622,753	-				-0.3
3,622,804	-				+1.5
3,622,842	+				+1.1
3,622,842	+				-0.6
3,622,842	+				+1.8
3,622,843	-				-0.8
3,623,030	+		PP_3194	0.24	-1.0
3,623,112	-		PP_3194	0.30	-0.2
3,623,195	-		PP_3194	0.36	-1.6
3,623,305	-		PP_3194	0.44	-1.2
3,623,409	+		PP_3194	0.52	-2.0
3,623,578	-		PP_3194	0.65	+0.3
3,624,089	-				+0.7
3,624,122	+				+0.4
3,624,123	-				-1.8
3,624,123	-				-1.0
3,624,271	+				+0.8
3,624,271	+				-0.1
3,624,272	-				-1.4
3,624,272	-				-0.6
3,624,442	-		PP_3195	0.17	+0.2
3,624,474	+		PP_3195	0.20	-1.1
3,624,670	+		PP_3195	0.35	+0.6
3,624,747	+		PP_3195	0.41	+2.4