Strain Fitness in Pseudomonas putida KT2440 around PP_3005

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,398,336	+				-0.3
3,398,356	+				-0.3
3,398,356	+				+0.5
3,398,356	+				-0.2
3,398,356	+				+1.0
3,398,356	+				+0.8
3,398,357	-				-0.3
3,398,357	-				-0.5
3,398,357	-				+0.6
3,398,413	-		PP_3003	0.16	-1.4
3,398,589	+		PP_3003	0.56	+0.4
3,398,589	+		PP_3003	0.56	-0.1
3,398,590	-		PP_3003	0.56	-0.7
3,398,595	+		PP_3003	0.58	-0.8
3,398,693	-		PP_3003	0.80	-0.4
3,398,743	-				+1.0
3,398,867	+				+2.4
3,398,867	+				-0.9
3,398,868	-				+1.8
3,398,868	-				+0.3
3,398,868	-				-0.6
3,398,868	-				+0.4
3,398,921	-				+0.0
3,398,940	+				+0.2
3,398,940	+				+0.2
3,398,940	+				-1.2
3,398,940	+				+0.8
3,398,941	-				-1.0
3,398,941	-				-0.1
3,398,941	-				+1.1
3,398,941	-				-0.2
3,398,941	-				-1.7
3,398,941	-				+0.3
3,398,943	+				-0.2
3,398,944	-				-1.4
3,398,944	-				-0.5
3,398,944	-				-0.6
3,399,034	-				+0.2
3,399,034	-				-0.2
3,399,205	+		PP_3004	0.71	+1.7
3,399,283	+				-1.8
3,399,283	+				-0.3
3,399,283	+				+2.4
3,399,472	-		PP_3005	0.20	-0.4
3,399,674	-		PP_3005	0.49	-1.7
3,399,697	+		PP_3005	0.52	-1.7
3,399,795	-		PP_3005	0.66	+0.6
3,399,948	+		PP_3005	0.88	-1.1
3,399,948	+		PP_3005	0.88	+0.0
3,399,948	+		PP_3005	0.88	-1.0
3,399,948	+		PP_3005	0.88	-0.4
3,399,949	-		PP_3005	0.88	-1.8
3,399,949	-		PP_3005	0.88	-0.8
3,399,949	-		PP_3005	0.88	-0.9
3,399,949	-		PP_3005	0.88	-1.1
3,399,979	-				-0.7
3,399,979	-				+0.4
3,400,133	+		PP_3006	0.20	+0.7
3,400,134	-		PP_3006	0.21	+0.8
3,400,274	-		PP_3006	0.46	-1.4
3,400,298	-		PP_3006	0.50	-2.2
3,400,324	-		PP_3006	0.55	+0.3
3,400,331	+		PP_3006	0.56	-0.9
3,400,331	+		PP_3006	0.56	+0.4
3,400,332	-		PP_3006	0.57	-1.2
3,400,332	-		PP_3006	0.57	-0.5
3,400,368	-		PP_3006	0.63	+0.7
3,400,485	-		PP_3006	0.85	-1.0
3,400,526	-				-0.3
3,400,590	-				-0.9
3,400,590	-				+0.2
3,400,636	+				-0.7
3,400,636	+				+0.5
3,400,657	+				-0.7
3,400,658	-				-0.1
3,400,658	-				-2.2
3,400,695	+				+0.4
3,400,696	-				+0.2
3,400,696	-				+0.9
3,400,696	-				-0.7
3,400,696	-				+2.4
3,400,696	-				-0.4
3,400,696	-				+0.9
3,400,696	-				-0.8
3,400,696	-				+0.7
3,400,707	+				-0.2
3,400,794	+		PP_3007	0.23	+0.8
3,400,794	+		PP_3007	0.23	+0.5
3,400,794	+		PP_3007	0.23	-1.2
3,400,795	-				+4.0
3,400,795	-		PP_3007	0.23	+1.9
3,400,795	-		PP_3007	0.23	+0.8
3,400,795	-		PP_3007	0.23	+0.5
3,400,795	-		PP_3007	0.23	-0.4
3,400,795	-		PP_3007	0.23	+0.5
3,400,930	+		PP_3007	0.49	-0.0
3,400,948	-		PP_3007	0.52	+0.3
3,400,948	-		PP_3007	0.52	-1.1