Strain Fitness in Pseudomonas putida KT2440 around PP_2980

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
3,377,746	+				+0.2
3,377,746	+				-2.1
3,377,747	-				-0.8
3,377,747	-				+0.2
3,377,748	+				+0.3
3,377,748	+				-0.5
3,377,749	-				-0.2
3,377,749	-				-0.1
3,377,749	-				-0.8
3,377,749	-				-0.3
3,377,829	-				-0.5
3,377,829	-				-0.7
3,377,836	+				-0.3
3,377,837	-				-0.9
3,377,844	-				+0.8
3,377,877	-				+0.4
3,377,877	-				+0.1
3,377,906	-				+1.2
3,377,990	+				-0.9
3,377,990	+				+0.0
3,377,991	-				+0.6
3,378,117	-		PP_2978	0.41	-1.5
3,378,117	-		PP_2978	0.41	+2.7
3,378,163	+		PP_2978	0.52	-1.0
3,378,275	+		PP_2978	0.80	+0.2
3,378,275	+		PP_2978	0.80	-1.1
3,378,276	-		PP_2978	0.81	+0.5
3,378,276	-		PP_2978	0.81	+0.3
3,378,291	-		PP_2978	0.84	+2.0
3,378,291	-		PP_2978	0.84	-0.4
3,378,349	+				-0.2
3,378,349	+				-0.4
3,378,350	-				-1.1
3,378,351	+				-0.3
3,378,351	+				+0.8
3,378,351	+				-0.7
3,378,351	+				+0.1
3,378,352	-				+2.0
3,378,479	+		PP_2979	0.41	-0.7
3,378,479	+		PP_2979	0.41	+1.4
3,378,503	+		PP_2979	0.47	-0.6
3,378,503	+		PP_2979	0.47	-1.4
3,378,504	-		PP_2979	0.47	+1.6
3,378,504	-		PP_2979	0.47	-1.1
3,378,525	-		PP_2979	0.52	-0.4
3,378,547	-		PP_2979	0.57	+0.0
3,378,606	-		PP_2979	0.71	-0.1
3,378,606	-		PP_2979	0.71	-0.4
3,378,617	+		PP_2979	0.73	+0.4
3,378,618	-		PP_2979	0.74	+0.0
3,378,618	-		PP_2979	0.74	-0.4
3,378,659	-		PP_2979	0.83	-0.0
3,378,659	-		PP_2979	0.83	-1.1
3,378,719	+				-0.0
3,378,735	+				-0.5
3,378,762	-		PP_2980	0.12	-0.7
3,378,813	+		PP_2980	0.33	+4.6
3,378,814	-		PP_2980	0.34	+0.2
3,378,835	+		PP_2980	0.43	-0.3
3,378,875	+		PP_2980	0.59	-2.2
3,378,908	-		PP_2980	0.73	-1.0
3,378,908	-		PP_2980	0.73	-1.3
3,378,908	-		PP_2980	0.73	-0.2
3,378,916	-		PP_2980	0.77	-0.4
3,379,014	+				-1.7
3,379,015	-				+0.4
3,379,066	+				-0.4
3,379,081	+				+0.3
3,379,115	+				+1.8
3,379,116	-				+0.5
3,379,116	-				-1.5
3,379,137	-				+1.6
3,379,178	+				+0.8
3,379,179	-				-1.3
3,379,233	+				-1.5
3,379,233	+				+0.3
3,379,255	+				-0.3
3,379,272	-				+0.2
3,379,303	+		PP_2981	0.13	-1.5
3,379,303	+		PP_2981	0.13	-0.3
3,379,304	-		PP_2981	0.13	+0.0
3,379,304	-		PP_2981	0.13	+0.0
3,379,307	+		PP_2981	0.13	+0.2
3,379,307	+		PP_2981	0.13	-0.4
3,379,307	+		PP_2981	0.13	-0.2
3,379,307	+		PP_2981	0.13	+1.2
3,379,307	+		PP_2981	0.13	-1.2
3,379,307	+		PP_2981	0.13	-1.2
3,379,307	+		PP_2981	0.13	-0.8
3,379,307	+		PP_2981	0.13	-0.3
3,379,307	+		PP_2981	0.13	-0.9
3,379,308	-		PP_2981	0.13	-0.6
3,379,308	-		PP_2981	0.13	+0.5
3,379,308	-		PP_2981	0.13	+0.1
3,379,308	-		PP_2981	0.13	+0.6
3,379,308	-		PP_2981	0.13	-0.1
3,379,308	-		PP_2981	0.13	+1.0
3,379,385	+		PP_2981	0.21	-0.7
3,379,386	-		PP_2981	0.22	+0.1
3,379,386	-		PP_2981	0.22	-1.0
3,379,400	-		PP_2981	0.23	+0.2
3,379,401	+		PP_2981	0.23	+0.1
3,379,541	+		PP_2981	0.37	+1.3
3,379,576	+		PP_2981	0.41	+1.0
3,379,577	-		PP_2981	0.41	+0.8
3,379,580	+		PP_2981	0.41	+0.7
3,379,581	-		PP_2981	0.42	+0.0
3,379,614	+		PP_2981	0.45	-0.4
3,379,614	+		PP_2981	0.45	-0.5
3,379,615	-		PP_2981	0.45	+0.0
3,379,663	-		PP_2981	0.50	+0.2
3,379,941	+		PP_2981	0.79	+0.3
3,379,942	-				+1.2
3,379,942	-		PP_2981	0.79	-1.8
3,379,942	-		PP_2981	0.79	+2.2
3,379,942	-		PP_2981	0.79	+0.7