Strain Fitness in Pseudomonas putida KT2440 around PP_2794
Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 3,182,617 | - | PP_2793 | 0.24 | -1.8 | |
| 3,183,009 | + | PP_2793 | 0.57 | -0.1 | |
| 3,183,056 | - | PP_2793 | 0.61 | +0.0 | |
| 3,183,439 | + | -0.3 | |||
| 3,183,439 | + | +0.1 | |||
| 3,183,820 | + | PP_2794 | 0.40 | -0.5 | |
| 3,183,988 | - | PP_2794 | 0.62 | -0.1 | |
| 3,184,084 | - | PP_2794 | 0.74 | -1.1 | |
| 3,184,278 | + | +0.5 | |||
| 3,184,297 | - | +0.3 | |||
| 3,184,315 | + | +1.6 | |||
| 3,184,319 | + | -1.1 | |||
| 3,184,319 | + | +0.4 | |||
| 3,184,320 | - | -0.2 | |||
| 3,184,320 | - | -0.1 | |||
| 3,184,320 | - | -1.1 | |||
| 3,184,320 | - | -0.8 | |||
| 3,184,320 | - | +1.5 | |||
| 3,184,320 | - | +0.7 | |||
| 3,184,320 | - | +1.4 | |||
| 3,184,320 | - | -0.3 | |||
| 3,184,320 | - | -1.3 | |||
| 3,184,460 | + | -1.3 | |||
| 3,184,484 | + | +2.4 | |||
| 3,184,485 | - | +0.5 | |||
| 3,184,722 | + | PP_2795 | 0.22 | -0.3 | |
| 3,184,850 | + | PP_2795 | 0.30 | +0.2 | |
| 3,184,851 | - | PP_2795 | 0.30 | +2.4 | |
| 3,184,932 | - | PP_2795 | 0.34 | -1.2 | |
| 3,184,932 | - | PP_2795 | 0.34 | +0.6 | |
| 3,184,932 | - | PP_2795 | 0.34 | +1.4 | |
| 3,184,989 | - | PP_2795 | 0.38 | +1.4 | |
| 3,185,086 | + | PP_2795 | 0.44 | +1.0 |
Or see this region's nucleotide sequence