Strain Fitness in Pseudomonas putida KT2440 around PP_2076

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,360,433	-		PP_2075	0.36	+1.1
2,360,538	-		PP_2075	0.45	+0.1
2,360,672	-		PP_2075	0.57	+2.4
2,360,726	-		PP_2075	0.62	-0.2
2,360,753	+		PP_2075	0.64	+0.0
2,360,754	-		PP_2075	0.64	+1.2
2,360,783	-		PP_2075	0.67	-0.5
2,360,784	+		PP_2075	0.67	+0.5
2,360,799	+		PP_2075	0.68	-0.8
2,360,863	+		PP_2075	0.74	-0.7
2,360,864	-		PP_2075	0.74	+0.5
2,360,910	+		PP_2075	0.78	-0.8
2,360,911	-		PP_2075	0.78	+0.3
2,360,913	+		PP_2075	0.78	-0.7
2,360,979	+		PP_2075	0.84	-0.2
2,360,979	+		PP_2075	0.84	+0.1
2,361,004	-		PP_2075	0.87	+0.0
2,361,047	+				-0.5
2,361,089	+				+0.2
2,361,089	+				+0.7
2,361,090	-				-0.1
2,361,090	-				-0.2
2,361,149	+				+0.4
2,361,150	-				+0.9
2,361,160	-				+1.9
2,361,160	-				+0.2
2,361,160	-				+1.4
2,361,160	-				+1.4
2,361,284	-				+1.9
2,361,284	-				-1.1
2,361,338	+				+0.9
2,361,342	+				+0.1
2,361,342	+				+1.2
2,361,343	-				-1.0
2,361,400	-				-0.3
2,361,478	-		PP_2076	0.12	-0.2
2,361,503	+		PP_2076	0.13	-1.1
2,361,504	-		PP_2076	0.13	-0.7
2,361,590	-		PP_2076	0.16	-1.0
2,362,145	-		PP_2076	0.36	-2.4
2,362,154	-		PP_2076	0.37	+0.1
2,362,154	-		PP_2076	0.37	-0.0
2,362,154	-		PP_2076	0.37	-1.2
2,363,324	-		PP_2076	0.80	-0.1
2,363,399	-		PP_2076	0.82	+0.5
2,363,399	-		PP_2076	0.82	-1.2
2,363,399	-		PP_2076	0.82	-1.1
2,363,489	-		PP_2076	0.86	-0.1
2,363,489	-		PP_2076	0.86	-0.5
2,363,537	-		PP_2076	0.88	+3.9
2,363,827	+				+0.5
2,363,827	+				-0.7
2,363,827	+				-1.1
2,363,827	+				+2.9
2,363,827	+				-0.9
2,363,827	+				-0.7
2,363,827	+				+0.2
2,363,828	-				+0.6
2,363,828	-				+0.3
2,363,828	-				+1.6
2,363,828	-				+0.0
2,363,828	-				-1.0
2,363,828	-				+0.1
2,363,829	+				-0.9
2,363,830	-				-1.0
2,363,830	-				+0.2
2,363,848	+				-1.7
2,363,849	-				-0.6
2,363,936	+				+0.4
2,363,936	+				-1.8
2,363,945	-				+1.6
2,364,071	+				+0.5
2,364,210	+				-0.7
2,364,283	-				-0.3
2,364,318	+				+0.8
2,364,319	-				-1.8
2,364,319	-				+0.5
2,364,343	-		PP_2077	0.11	+0.4
2,364,488	+		PP_2077	0.23	+0.8
2,364,488	+		PP_2077	0.23	-0.7
2,364,489	-		PP_2077	0.23	-0.0
2,364,537	+		PP_2077	0.27	-0.9
2,364,537	+		PP_2077	0.27	-2.4
2,364,578	-		PP_2077	0.30	-0.1
2,364,589	+		PP_2077	0.31	-0.7
2,364,589	+		PP_2077	0.31	-0.2
2,364,590	-		PP_2077	0.31	-1.1
2,364,633	+		PP_2077	0.34	-1.0
2,364,634	-		PP_2077	0.34	-0.8
2,364,876	+		PP_2077	0.54	+0.2