Strain Fitness in Pseudomonas putida KT2440 around PP_2043

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
2,322,382	+		PP_2042	0.50	-1.7
2,322,382	+		PP_2042	0.50	+1.3
2,322,382	+		PP_2042	0.50	-1.3
2,322,383	-		PP_2042	0.50	-0.6
2,322,649	+		PP_2042	0.64	+1.1
2,322,649	+		PP_2042	0.64	+0.3
2,322,650	-		PP_2042	0.64	-2.0
2,322,762	+		PP_2042	0.70	-0.4
2,322,763	-		PP_2042	0.71	-0.0
2,322,810	-		PP_2042	0.73	+0.1
2,322,863	+		PP_2042	0.76	+0.1
2,322,884	-		PP_2042	0.77	+2.0
2,322,884	-		PP_2042	0.77	+0.1
2,322,907	+		PP_2042	0.78	-0.0
2,322,908	-		PP_2042	0.78	+1.4
2,323,063	+		PP_2042	0.87	+1.1
2,323,210	+				-0.1
2,323,210	+				-1.0
2,323,210	+				+0.1
2,323,211	-				-0.5
2,323,211	-				+0.2
2,323,228	+				+0.1
2,323,465	+		PP_2043	0.11	-0.4
2,323,533	-		PP_2043	0.17	+1.1
2,323,553	+		PP_2043	0.18	+2.4
2,323,824	-		PP_2043	0.38	-0.0
2,323,824	-		PP_2043	0.38	-0.9
2,323,943	+		PP_2043	0.47	-0.8
2,323,943	+		PP_2043	0.47	+0.3
2,323,944	-		PP_2043	0.47	-0.2
2,323,946	+		PP_2043	0.48	-0.4
2,323,947	-		PP_2043	0.48	+0.3
2,323,947	-		PP_2043	0.48	-0.1
2,324,048	+		PP_2043	0.55	-0.8
2,324,049	-		PP_2043	0.55	-1.9
2,324,249	+		PP_2043	0.70	+0.9
2,324,297	+		PP_2043	0.74	+0.3
2,324,353	+		PP_2043	0.78	-0.1
2,324,353	+		PP_2043	0.78	-2.0
2,324,353	+		PP_2043	0.78	+0.7
2,324,353	+		PP_2043	0.78	-0.5
2,324,353	+		PP_2043	0.78	+0.2
2,324,353	+		PP_2043	0.78	-0.6
2,324,353	+		PP_2043	0.78	+0.2
2,324,353	+		PP_2043	0.78	+0.3
2,324,354	-		PP_2043	0.78	+0.6
2,324,354	-		PP_2043	0.78	+0.5
2,324,354	-		PP_2043	0.78	-1.3
2,324,354	-		PP_2043	0.78	-1.7
2,324,354	-		PP_2043	0.78	+0.9
2,324,354	-		PP_2043	0.78	+0.8
2,324,354	-		PP_2043	0.78	+0.3
2,324,354	-		PP_2043	0.78	+1.7
2,324,354	-		PP_2043	0.78	-0.1
2,324,354	-		PP_2043	0.78	+0.3
2,324,354	-		PP_2043	0.78	+0.1
2,324,354	-		PP_2043	0.78	+0.8
2,324,356	-		PP_2043	0.78	-1.3
2,324,357	+		PP_2043	0.79	+1.1
2,324,357	+		PP_2043	0.79	-0.1
2,324,358	-		PP_2043	0.79	-0.0
2,324,358	-		PP_2043	0.79	+0.6
2,324,376	-		PP_2043	0.80	+0.1
2,324,417	+		PP_2043	0.83	+0.2
2,324,510	+				-0.7
2,324,511	-				+0.2
2,324,531	+				+0.4
2,324,733	+		PP_2044	0.24	-0.9
2,324,733	+		PP_2044	0.24	+0.8
2,325,125	+		PP_5499	0.36	+0.7
2,325,125	+		PP_5499	0.36	-0.3
2,325,125	+		PP_5499	0.36	+0.0
2,325,125	+		PP_5499	0.36	-0.2
2,325,125	+		PP_5499	0.36	-0.1
2,325,126	-		PP_5499	0.36	+0.6
2,325,513	+				-0.6
2,325,514	-				+0.4
2,325,514	-				-1.1
2,325,581	-		PP_2045	0.12	-0.6
2,325,640	+				+0.5
2,325,641	-		PP_2045	0.15	-0.4
2,325,641	-		PP_2045	0.15	+0.7
2,325,641	-		PP_2045	0.15	-0.7