Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1743 and PP_1744 are separated by 261 nucleotides PP_1744 and PP_1745 are separated by 206 nucleotides PP_1745 and PP_1746 are separated by 120 nucleotides PP_1746 and PP_1747 are separated by 14 nucleotides PP_1747 and PP_1748 are separated by 83 nucleotides
PP_1743: PP_1743 - acetate permease, at 1,942,835 to 1,944,499
_1743
PP_1744: PP_1744 - conserved exported protein of unknown function, at 1,944,761 to 1,944,913
_1744
PP_1745: PP_1745 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,945,120 to 1,945,929
_1745
PP_1746: PP_1746 - putative carbon storage regulator, csrA-like, at 1,946,050 to 1,946,229
_1746
PP_1747: PP_1747 - conserved protein of unknown function, UPF0270 family, at 1,946,244 to 1,946,471
_1747
PP_1748: PP_1748 - conserved protein of unknown function, at 1,946,555 to 1,947,739
_1748
Position (kb)
1945
1946 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1944.204 kb on - strand, within PP_1743 at 1944.204 kb on - strand, within PP_1743 at 1944.329 kb on + strand, within PP_1743 at 1944.335 kb on + strand at 1944.336 kb on - strand at 1944.342 kb on - strand at 1944.497 kb on + strand at 1944.497 kb on + strand at 1944.497 kb on + strand at 1944.497 kb on + strand at 1944.498 kb on - strand at 1944.661 kb on + strand at 1944.662 kb on - strand at 1944.668 kb on + strand at 1944.668 kb on + strand at 1944.669 kb on - strand at 1944.749 kb on - strand at 1944.764 kb on + strand at 1944.764 kb on + strand at 1944.764 kb on + strand at 1944.764 kb on + strand at 1944.764 kb on + strand at 1944.764 kb on + strand at 1944.764 kb on + strand at 1944.765 kb on - strand at 1944.782 kb on + strand, within PP_1744 at 1944.782 kb on + strand, within PP_1744 at 1944.782 kb on + strand, within PP_1744 at 1944.782 kb on + strand, within PP_1744 at 1944.785 kb on + strand at 1944.785 kb on + strand, within PP_1744 at 1944.785 kb on + strand, within PP_1744 at 1944.786 kb on - strand, within PP_1744 at 1944.831 kb on + strand, within PP_1744 at 1944.898 kb on + strand at 1944.933 kb on + strand at 1944.992 kb on - strand at 1945.080 kb on + strand at 1945.081 kb on - strand at 1945.081 kb on - strand at 1945.306 kb on + strand, within PP_1745 at 1945.306 kb on + strand, within PP_1745 at 1945.309 kb on - strand, within PP_1745 at 1945.309 kb on - strand, within PP_1745 at 1945.309 kb on - strand, within PP_1745 at 1945.516 kb on - strand, within PP_1745 at 1945.516 kb on - strand at 1945.568 kb on - strand, within PP_1745 at 1945.568 kb on - strand, within PP_1745 at 1945.774 kb on + strand, within PP_1745 at 1945.774 kb on + strand, within PP_1745 at 1945.774 kb on + strand, within PP_1745 at 1945.774 kb on + strand, within PP_1745 at 1945.774 kb on + strand, within PP_1745 at 1945.774 kb on + strand, within PP_1745 at 1945.776 kb on + strand, within PP_1745 at 1945.777 kb on - strand, within PP_1745 at 1945.873 kb on + strand at 1945.874 kb on - strand at 1945.939 kb on - strand at 1945.939 kb on - strand at 1945.944 kb on + strand at 1945.944 kb on + strand at 1945.944 kb on + strand at 1945.944 kb on + strand at 1945.945 kb on - strand at 1945.945 kb on - strand at 1945.945 kb on - strand at 1945.945 kb on - strand at 1945.945 kb on - strand at 1945.945 kb on - strand at 1945.945 kb on - strand at 1945.948 kb on + strand at 1945.948 kb on + strand at 1945.949 kb on - strand at 1945.949 kb on - strand at 1945.949 kb on - strand at 1945.960 kb on + strand at 1945.961 kb on - strand at 1945.961 kb on - strand at 1945.961 kb on - strand at 1945.987 kb on + strand at 1945.988 kb on - strand at 1945.994 kb on + strand at 1945.995 kb on - strand at 1946.191 kb on + strand, within PP_1746 at 1946.356 kb on - strand, within PP_1747 at 1946.459 kb on - strand at 1946.492 kb on - strand at 1946.496 kb on - strand at 1946.506 kb on + strand at 1946.506 kb on + strand at 1946.556 kb on + strand at 1946.656 kb on + strand at 1946.657 kb on - strand at 1946.657 kb on - strand at 1946.704 kb on - strand, within PP_1748 at 1946.704 kb on - strand, within PP_1748 at 1946.804 kb on - strand, within PP_1748 at 1946.919 kb on + strand, within PP_1748 at 1946.919 kb on + strand, within PP_1748 at 1946.926 kb on - strand, within PP_1748
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,944,204 - PP_1743 0.82 +2.0 1,944,204 - PP_1743 0.82 -0.5 1,944,329 + PP_1743 0.90 +0.8 1,944,335 + +0.5 1,944,336 - +1.6 1,944,342 - +2.4 1,944,497 + +0.3 1,944,497 + +0.8 1,944,497 + -0.2 1,944,497 + +3.0 1,944,498 - -1.0 1,944,661 + +0.6 1,944,662 - -0.1 1,944,668 + +0.5 1,944,668 + +0.3 1,944,669 - -0.5 1,944,749 - +0.4 1,944,764 + +1.2 1,944,764 + +0.4 1,944,764 + -1.2 1,944,764 + +1.0 1,944,764 + -0.7 1,944,764 + -0.2 1,944,764 + +1.1 1,944,765 - +0.6 1,944,782 + PP_1744 0.14 +0.4 1,944,782 + PP_1744 0.14 +1.2 1,944,782 + PP_1744 0.14 +0.0 1,944,782 + PP_1744 0.14 -1.8 1,944,785 + +4.0 1,944,785 + PP_1744 0.16 +1.2 1,944,785 + PP_1744 0.16 -0.4 1,944,786 - PP_1744 0.16 -0.4 1,944,831 + PP_1744 0.46 +0.2 1,944,898 + +1.2 1,944,933 + +4.0 1,944,992 - -0.7 1,945,080 + -1.2 1,945,081 - -1.2 1,945,081 - +0.3 1,945,306 + PP_1745 0.23 +0.2 1,945,306 + PP_1745 0.23 -0.9 1,945,309 - PP_1745 0.23 +1.1 1,945,309 - PP_1745 0.23 -0.4 1,945,309 - PP_1745 0.23 -1.2 1,945,516 - PP_1745 0.49 +2.4 1,945,516 - +1.0 1,945,568 - PP_1745 0.55 -0.7 1,945,568 - PP_1745 0.55 -1.2 1,945,774 + PP_1745 0.81 -0.2 1,945,774 + PP_1745 0.81 -1.4 1,945,774 + PP_1745 0.81 -1.0 1,945,774 + PP_1745 0.81 -1.6 1,945,774 + PP_1745 0.81 -1.7 1,945,774 + PP_1745 0.81 -0.5 1,945,776 + PP_1745 0.81 -0.4 1,945,777 - PP_1745 0.81 +0.5 1,945,873 + +1.6 1,945,874 - +0.4 1,945,939 - -1.5 1,945,939 - -0.6 1,945,944 + +0.6 1,945,944 + -1.3 1,945,944 + -0.1 1,945,944 + +0.1 1,945,945 - -1.7 1,945,945 - -0.6 1,945,945 - +0.4 1,945,945 - +0.0 1,945,945 - -0.1 1,945,945 - +0.1 1,945,945 - +1.0 1,945,948 + -0.8 1,945,948 + +0.5 1,945,949 - +0.8 1,945,949 - +0.1 1,945,949 - +0.5 1,945,960 + -0.4 1,945,961 - -1.2 1,945,961 - +0.6 1,945,961 - +1.6 1,945,987 + +4.0 1,945,988 - +0.3 1,945,994 + -1.7 1,945,995 - -0.0 1,946,191 + PP_1746 0.78 -1.3 1,946,356 - PP_1747 0.49 -0.4 1,946,459 - -0.1 1,946,492 - -0.3 1,946,496 - +0.6 1,946,506 + +0.1 1,946,506 + +1.1 1,946,556 + +4.0 1,946,656 + +0.5 1,946,657 - +0.7 1,946,657 - +0.3 1,946,704 - PP_1748 0.13 +0.2 1,946,704 - PP_1748 0.13 +0.2 1,946,804 - PP_1748 0.21 -0.5 1,946,919 + PP_1748 0.31 -0.2 1,946,919 + PP_1748 0.31 +1.7 1,946,926 - PP_1748 0.31 -0.9
Or see this region's nucleotide sequence