Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1665 and PP_1666 are separated by 320 nucleotides PP_1666 and PP_1667 are separated by 12 nucleotides
PP_1665: PP_1665 - Phosphoribosylformylglycinamidine cyclo-ligase, at 1,860,936 to 1,861,994
_1665
PP_1666: PP_1666 - conserved exported protein of unknown function, at 1,862,315 to 1,863,382
_1666
PP_1667: PP_1667 - putative Transporter, at 1,863,395 to 1,864,468
_1667
Position (kb)
1862
1863
1864 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1862.009 kb on - strand at 1862.155 kb on + strand at 1862.156 kb on - strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.200 kb on + strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.201 kb on - strand at 1862.202 kb on + strand at 1862.202 kb on + strand at 1862.203 kb on - strand at 1862.203 kb on - strand at 1862.281 kb on + strand at 1862.330 kb on - strand at 1862.397 kb on - strand at 1862.397 kb on - strand at 1862.397 kb on - strand at 1862.467 kb on + strand, within PP_1666 at 1862.468 kb on - strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.497 kb on + strand, within PP_1666 at 1862.614 kb on + strand, within PP_1666 at 1862.615 kb on - strand, within PP_1666 at 1862.883 kb on - strand, within PP_1666 at 1862.907 kb on + strand, within PP_1666 at 1862.908 kb on - strand, within PP_1666 at 1862.908 kb on - strand, within PP_1666 at 1862.908 kb on - strand, within PP_1666 at 1863.056 kb on + strand, within PP_1666 at 1863.056 kb on + strand, within PP_1666 at 1863.056 kb on + strand, within PP_1666 at 1863.056 kb on + strand, within PP_1666 at 1863.057 kb on - strand, within PP_1666 at 1863.062 kb on - strand, within PP_1666 at 1863.123 kb on - strand, within PP_1666 at 1863.152 kb on + strand, within PP_1666 at 1863.202 kb on + strand, within PP_1666 at 1863.222 kb on + strand, within PP_1666 at 1863.222 kb on + strand, within PP_1666 at 1863.222 kb on + strand, within PP_1666 at 1863.223 kb on - strand, within PP_1666 at 1863.223 kb on - strand, within PP_1666 at 1863.223 kb on - strand at 1863.223 kb on - strand, within PP_1666 at 1863.380 kb on + strand at 1863.380 kb on + strand at 1863.456 kb on - strand at 1863.458 kb on - strand at 1863.469 kb on + strand at 1863.469 kb on + strand at 1863.469 kb on + strand at 1863.469 kb on + strand at 1863.469 kb on + strand at 1863.470 kb on - strand at 1863.470 kb on - strand at 1863.507 kb on - strand, within PP_1667 at 1863.799 kb on + strand, within PP_1667 at 1863.865 kb on + strand, within PP_1667 at 1863.866 kb on - strand, within PP_1667 at 1863.893 kb on + strand, within PP_1667 at 1863.893 kb on + strand, within PP_1667 at 1863.893 kb on + strand, within PP_1667 at 1863.894 kb on - strand, within PP_1667 at 1863.894 kb on - strand, within PP_1667 at 1863.894 kb on - strand, within PP_1667 at 1863.894 kb on - strand, within PP_1667 at 1864.052 kb on + strand, within PP_1667 at 1864.052 kb on + strand, within PP_1667 at 1864.053 kb on - strand, within PP_1667 at 1864.053 kb on - strand, within PP_1667 at 1864.053 kb on - strand, within PP_1667 at 1864.053 kb on - strand, within PP_1667 at 1864.134 kb on - strand, within PP_1667 at 1864.134 kb on - strand, within PP_1667 at 1864.256 kb on - strand, within PP_1667
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,862,009 - +0.1 1,862,155 + +0.4 1,862,156 - +1.6 1,862,200 + -0.8 1,862,200 + +0.0 1,862,200 + -1.7 1,862,200 + -1.0 1,862,200 + -0.5 1,862,200 + +0.4 1,862,200 + +0.2 1,862,201 - +1.0 1,862,201 - -1.1 1,862,201 - -0.2 1,862,201 - +1.1 1,862,201 - -0.9 1,862,202 + +0.0 1,862,202 + -0.5 1,862,203 - +0.3 1,862,203 - +0.3 1,862,281 + +0.1 1,862,330 - -0.5 1,862,397 - -0.2 1,862,397 - +3.0 1,862,397 - +1.3 1,862,467 + PP_1666 0.14 -0.0 1,862,468 - PP_1666 0.14 +0.6 1,862,497 + PP_1666 0.17 -1.5 1,862,497 + PP_1666 0.17 -1.8 1,862,497 + PP_1666 0.17 -1.4 1,862,497 + PP_1666 0.17 -1.1 1,862,614 + PP_1666 0.28 -0.3 1,862,615 - PP_1666 0.28 -0.8 1,862,883 - PP_1666 0.53 -2.0 1,862,907 + PP_1666 0.55 +0.1 1,862,908 - PP_1666 0.56 +0.9 1,862,908 - PP_1666 0.56 -1.1 1,862,908 - PP_1666 0.56 -0.6 1,863,056 + PP_1666 0.69 +0.9 1,863,056 + PP_1666 0.69 -1.3 1,863,056 + PP_1666 0.69 -0.2 1,863,056 + PP_1666 0.69 -0.5 1,863,057 - PP_1666 0.69 -1.1 1,863,062 - PP_1666 0.70 +0.4 1,863,123 - PP_1666 0.76 -0.7 1,863,152 + PP_1666 0.78 +0.8 1,863,202 + PP_1666 0.83 -1.0 1,863,222 + PP_1666 0.85 +0.5 1,863,222 + PP_1666 0.85 -1.1 1,863,222 + PP_1666 0.85 -0.2 1,863,223 - PP_1666 0.85 +0.6 1,863,223 - PP_1666 0.85 -1.0 1,863,223 - +1.6 1,863,223 - PP_1666 0.85 -1.2 1,863,380 + +0.7 1,863,380 + -0.7 1,863,456 - -0.6 1,863,458 - +1.3 1,863,469 + -0.2 1,863,469 + -1.1 1,863,469 + +1.8 1,863,469 + +0.9 1,863,469 + -0.5 1,863,470 - +0.1 1,863,470 - +0.3 1,863,507 - PP_1667 0.10 +0.1 1,863,799 + PP_1667 0.38 +1.0 1,863,865 + PP_1667 0.44 -0.2 1,863,866 - PP_1667 0.44 +0.0 1,863,893 + PP_1667 0.46 +1.5 1,863,893 + PP_1667 0.46 -0.2 1,863,893 + PP_1667 0.46 +0.2 1,863,894 - PP_1667 0.46 +0.7 1,863,894 - PP_1667 0.46 -0.7 1,863,894 - PP_1667 0.46 -0.3 1,863,894 - PP_1667 0.46 -1.0 1,864,052 + PP_1667 0.61 -1.0 1,864,052 + PP_1667 0.61 -0.8 1,864,053 - PP_1667 0.61 -1.9 1,864,053 - PP_1667 0.61 +0.1 1,864,053 - PP_1667 0.61 -0.7 1,864,053 - PP_1667 0.61 -0.4 1,864,134 - PP_1667 0.69 +2.4 1,864,134 - PP_1667 0.69 -0.0 1,864,256 - PP_1667 0.80 -1.4
Or see this region's nucleotide sequence