Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1570 and PP_1571 overlap by 8 nucleotides PP_1571 and PP_1572 overlap by 4 nucleotides PP_1572 and PP_1573 are separated by 65 nucleotides PP_1573 and PP_1574 are separated by 8 nucleotides PP_1574 and PP_1575 are separated by 62 nucleotides PP_1575 and PP_1576 are separated by 61 nucleotides
PP_1570: PP_1570 - Head-tail adaptor, at 1,762,843 to 1,763,184
_1570
PP_1571: PP_1571 - conserved protein of unknown function, at 1,763,177 to 1,763,662
_1571
PP_1572: PP_1572 - conserved protein of unknown function, at 1,763,659 to 1,764,027
_1572
PP_1573: PP_1573 - putative Major tail protein, at 1,764,093 to 1,764,827
_1573
PP_1574: PP_1574 - conserved protein of unknown function, at 1,764,836 to 1,765,306
_1574
PP_1575: PP_1575 - conserved protein of unknown function, at 1,765,369 to 1,765,530
_1575
PP_1576: PP_1576 - putative Immunity protein, at 1,765,592 to 1,765,915
_1576
Position (kb)
1764
1765 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1763.095 kb on + strand, within PP_1570 at 1763.095 kb on + strand, within PP_1570 at 1763.096 kb on - strand, within PP_1570 at 1763.096 kb on - strand, within PP_1570 at 1763.153 kb on + strand at 1763.153 kb on + strand at 1763.154 kb on - strand at 1763.154 kb on - strand at 1763.505 kb on + strand, within PP_1571 at 1763.546 kb on - strand, within PP_1571 at 1763.588 kb on - strand, within PP_1571 at 1763.604 kb on - strand, within PP_1571 at 1763.741 kb on - strand, within PP_1572 at 1763.779 kb on + strand, within PP_1572 at 1763.779 kb on + strand, within PP_1572 at 1763.780 kb on - strand, within PP_1572 at 1763.780 kb on - strand, within PP_1572 at 1763.819 kb on - strand, within PP_1572 at 1763.819 kb on - strand, within PP_1572 at 1763.938 kb on + strand, within PP_1572 at 1763.938 kb on + strand, within PP_1572 at 1763.938 kb on + strand, within PP_1572 at 1763.938 kb on + strand, within PP_1572 at 1763.938 kb on + strand, within PP_1572 at 1763.939 kb on - strand, within PP_1572 at 1763.987 kb on - strand, within PP_1572 at 1764.046 kb on + strand at 1764.123 kb on + strand at 1764.123 kb on + strand at 1764.123 kb on + strand at 1764.124 kb on - strand at 1764.153 kb on - strand at 1764.157 kb on + strand at 1764.157 kb on + strand at 1764.157 kb on + strand at 1764.158 kb on - strand at 1764.259 kb on - strand, within PP_1573 at 1764.259 kb on - strand, within PP_1573 at 1764.410 kb on + strand, within PP_1573 at 1764.411 kb on - strand, within PP_1573 at 1764.411 kb on - strand, within PP_1573 at 1764.411 kb on - strand, within PP_1573 at 1764.411 kb on - strand, within PP_1573 at 1764.475 kb on - strand, within PP_1573 at 1764.591 kb on + strand, within PP_1573 at 1764.594 kb on - strand, within PP_1573 at 1764.594 kb on - strand, within PP_1573 at 1764.594 kb on - strand, within PP_1573 at 1764.606 kb on - strand, within PP_1573 at 1764.647 kb on + strand, within PP_1573 at 1764.647 kb on + strand, within PP_1573 at 1764.718 kb on - strand, within PP_1573 at 1764.944 kb on + strand, within PP_1574 at 1764.944 kb on + strand, within PP_1574 at 1764.944 kb on + strand, within PP_1574 at 1764.945 kb on - strand, within PP_1574 at 1764.945 kb on - strand, within PP_1574 at 1764.945 kb on - strand, within PP_1574 at 1765.000 kb on + strand, within PP_1574 at 1765.000 kb on + strand, within PP_1574 at 1765.085 kb on - strand, within PP_1574 at 1765.182 kb on + strand, within PP_1574 at 1765.183 kb on - strand, within PP_1574 at 1765.183 kb on - strand, within PP_1574 at 1765.183 kb on - strand, within PP_1574 at 1765.337 kb on - strand at 1765.337 kb on - strand at 1765.450 kb on + strand, within PP_1575 at 1765.451 kb on - strand, within PP_1575 at 1765.518 kb on + strand at 1765.519 kb on - strand at 1765.519 kb on - strand at 1765.519 kb on - strand at 1765.545 kb on + strand at 1765.559 kb on + strand at 1765.560 kb on - strand at 1765.560 kb on - strand at 1765.616 kb on + strand at 1765.616 kb on + strand at 1765.617 kb on - strand at 1765.633 kb on - strand, within PP_1576 at 1765.646 kb on + strand, within PP_1576 at 1765.646 kb on + strand, within PP_1576 at 1765.691 kb on + strand, within PP_1576 at 1765.759 kb on - strand, within PP_1576 at 1765.819 kb on + strand, within PP_1576 at 1765.820 kb on - strand, within PP_1576 at 1765.823 kb on + strand, within PP_1576 at 1765.823 kb on + strand, within PP_1576
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,763,095 + PP_1570 0.74 -0.0 1,763,095 + PP_1570 0.74 -1.4 1,763,096 - PP_1570 0.74 -0.9 1,763,096 - PP_1570 0.74 -0.8 1,763,153 + -0.0 1,763,153 + -0.4 1,763,154 - -0.7 1,763,154 - -0.9 1,763,505 + PP_1571 0.67 +0.2 1,763,546 - PP_1571 0.76 -0.9 1,763,588 - PP_1571 0.85 -0.3 1,763,604 - PP_1571 0.88 +0.5 1,763,741 - PP_1572 0.22 +0.9 1,763,779 + PP_1572 0.33 -0.4 1,763,779 + PP_1572 0.33 -0.3 1,763,780 - PP_1572 0.33 +0.5 1,763,780 - PP_1572 0.33 -0.9 1,763,819 - PP_1572 0.43 -0.9 1,763,819 - PP_1572 0.43 -1.4 1,763,938 + PP_1572 0.76 +0.8 1,763,938 + PP_1572 0.76 +0.6 1,763,938 + PP_1572 0.76 +0.3 1,763,938 + PP_1572 0.76 +1.4 1,763,938 + PP_1572 0.76 +0.0 1,763,939 - PP_1572 0.76 +1.3 1,763,987 - PP_1572 0.89 -2.0 1,764,046 + -1.1 1,764,123 + +0.5 1,764,123 + -0.8 1,764,123 + -0.0 1,764,124 - -1.7 1,764,153 - -0.9 1,764,157 + +0.6 1,764,157 + +0.4 1,764,157 + -0.4 1,764,158 - +0.8 1,764,259 - PP_1573 0.23 -0.1 1,764,259 - PP_1573 0.23 -2.5 1,764,410 + PP_1573 0.43 -0.3 1,764,411 - PP_1573 0.43 -0.1 1,764,411 - PP_1573 0.43 -0.7 1,764,411 - PP_1573 0.43 -0.4 1,764,411 - PP_1573 0.43 -1.5 1,764,475 - PP_1573 0.52 -1.2 1,764,591 + PP_1573 0.68 -0.4 1,764,594 - PP_1573 0.68 -0.1 1,764,594 - PP_1573 0.68 -0.1 1,764,594 - PP_1573 0.68 -0.5 1,764,606 - PP_1573 0.70 -1.4 1,764,647 + PP_1573 0.75 -0.4 1,764,647 + PP_1573 0.75 -1.2 1,764,718 - PP_1573 0.85 -0.0 1,764,944 + PP_1574 0.23 -1.8 1,764,944 + PP_1574 0.23 -1.1 1,764,944 + PP_1574 0.23 +0.1 1,764,945 - PP_1574 0.23 -0.7 1,764,945 - PP_1574 0.23 -0.7 1,764,945 - PP_1574 0.23 +0.5 1,765,000 + PP_1574 0.35 -0.9 1,765,000 + PP_1574 0.35 +0.9 1,765,085 - PP_1574 0.53 -0.8 1,765,182 + PP_1574 0.73 -0.4 1,765,183 - PP_1574 0.74 -0.1 1,765,183 - PP_1574 0.74 +0.1 1,765,183 - PP_1574 0.74 -0.0 1,765,337 - -0.5 1,765,337 - +0.7 1,765,450 + PP_1575 0.50 +0.5 1,765,451 - PP_1575 0.51 -1.6 1,765,518 + -0.8 1,765,519 - +1.2 1,765,519 - +0.5 1,765,519 - +0.1 1,765,545 + -0.5 1,765,559 + -1.0 1,765,560 - -0.4 1,765,560 - +1.4 1,765,616 + +0.2 1,765,616 + +0.0 1,765,617 - -0.6 1,765,633 - PP_1576 0.13 +0.8 1,765,646 + PP_1576 0.17 -0.4 1,765,646 + PP_1576 0.17 -1.3 1,765,691 + PP_1576 0.31 +1.8 1,765,759 - PP_1576 0.52 -1.2 1,765,819 + PP_1576 0.70 -1.0 1,765,820 - PP_1576 0.70 +0.1 1,765,823 + PP_1576 0.71 +0.1 1,765,823 + PP_1576 0.71 -0.0
Or see this region's nucleotide sequence