Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1517 and PP_1518 are separated by 48 nucleotides PP_1518 and PP_1519 are separated by 33 nucleotides PP_1519 and PP_1520 are separated by 76 nucleotides
PP_1517: PP_1517 - RND efflux transporter, at 1,721,368 to 1,724,433
_1517
PP_1518: PP_1518 - conserved exported protein of unknown function, at 1,724,482 to 1,725,168
_1518
PP_1519: PP_1519 - putative Lipoprotein, at 1,725,202 to 1,725,600
_1519
PP_1520: PP_1520 - glycerol-3-phosphate acyltransferase, at 1,725,677 to 1,728,163
_1520
Position (kb)
1725
1726 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1724.202 kb on - strand at 1724.202 kb on - strand at 1724.313 kb on + strand at 1724.314 kb on - strand at 1724.324 kb on - strand at 1724.376 kb on + strand at 1724.376 kb on + strand at 1724.377 kb on - strand at 1724.377 kb on - strand at 1724.377 kb on - strand at 1724.377 kb on - strand at 1724.377 kb on - strand at 1724.401 kb on + strand at 1724.453 kb on + strand at 1724.603 kb on + strand, within PP_1518 at 1724.604 kb on - strand, within PP_1518 at 1724.604 kb on - strand, within PP_1518 at 1724.669 kb on + strand, within PP_1518 at 1724.669 kb on + strand, within PP_1518 at 1724.670 kb on - strand, within PP_1518 at 1724.670 kb on - strand, within PP_1518 at 1724.670 kb on - strand, within PP_1518 at 1724.670 kb on - strand, within PP_1518 at 1724.760 kb on - strand, within PP_1518 at 1725.194 kb on + strand at 1725.195 kb on - strand at 1725.195 kb on - strand at 1725.195 kb on - strand at 1725.230 kb on - strand at 1725.320 kb on - strand, within PP_1519 at 1725.320 kb on - strand, within PP_1519 at 1725.320 kb on - strand, within PP_1519 at 1725.320 kb on - strand, within PP_1519 at 1725.359 kb on + strand, within PP_1519 at 1725.359 kb on + strand at 1725.359 kb on + strand, within PP_1519 at 1725.359 kb on + strand, within PP_1519 at 1725.359 kb on + strand, within PP_1519 at 1725.360 kb on - strand, within PP_1519 at 1725.360 kb on - strand, within PP_1519 at 1725.360 kb on - strand, within PP_1519 at 1725.360 kb on - strand, within PP_1519 at 1725.504 kb on - strand, within PP_1519 at 1725.504 kb on - strand, within PP_1519 at 1725.755 kb on + strand at 1725.756 kb on - strand at 1726.011 kb on + strand, within PP_1520 at 1726.011 kb on + strand, within PP_1520 at 1726.012 kb on - strand, within PP_1520 at 1726.096 kb on - strand, within PP_1520 at 1726.230 kb on + strand, within PP_1520 at 1726.231 kb on - strand, within PP_1520 at 1726.231 kb on - strand, within PP_1520 at 1726.231 kb on - strand, within PP_1520 at 1726.231 kb on - strand, within PP_1520 at 1726.326 kb on + strand at 1726.326 kb on + strand, within PP_1520 at 1726.327 kb on - strand, within PP_1520 at 1726.329 kb on + strand, within PP_1520 at 1726.330 kb on - strand, within PP_1520 at 1726.330 kb on - strand, within PP_1520 at 1726.498 kb on - strand, within PP_1520
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,724,202 - -0.3 1,724,202 - -1.1 1,724,313 + -0.0 1,724,314 - +0.5 1,724,324 - +0.8 1,724,376 + -0.5 1,724,376 + +1.0 1,724,377 - -0.6 1,724,377 - +0.8 1,724,377 - -0.8 1,724,377 - -0.3 1,724,377 - +0.0 1,724,401 + -0.7 1,724,453 + +2.7 1,724,603 + PP_1518 0.18 -0.2 1,724,604 - PP_1518 0.18 +0.4 1,724,604 - PP_1518 0.18 -0.6 1,724,669 + PP_1518 0.27 -0.2 1,724,669 + PP_1518 0.27 -0.2 1,724,670 - PP_1518 0.27 -0.2 1,724,670 - PP_1518 0.27 -0.5 1,724,670 - PP_1518 0.27 -0.5 1,724,670 - PP_1518 0.27 +0.4 1,724,760 - PP_1518 0.40 -1.1 1,725,194 + -0.6 1,725,195 - +0.1 1,725,195 - -0.5 1,725,195 - -0.6 1,725,230 - +1.8 1,725,320 - PP_1519 0.30 +0.1 1,725,320 - PP_1519 0.30 -1.5 1,725,320 - PP_1519 0.30 -0.3 1,725,320 - PP_1519 0.30 +1.4 1,725,359 + PP_1519 0.39 +1.5 1,725,359 + +1.6 1,725,359 + PP_1519 0.39 -0.7 1,725,359 + PP_1519 0.39 -1.0 1,725,359 + PP_1519 0.39 +2.0 1,725,360 - PP_1519 0.40 +1.0 1,725,360 - PP_1519 0.40 -1.0 1,725,360 - PP_1519 0.40 +0.6 1,725,360 - PP_1519 0.40 +2.4 1,725,504 - PP_1519 0.76 +2.1 1,725,504 - PP_1519 0.76 -0.7 1,725,755 + -2.0 1,725,756 - +0.5 1,726,011 + PP_1520 0.13 +0.7 1,726,011 + PP_1520 0.13 +0.4 1,726,012 - PP_1520 0.13 -0.1 1,726,096 - PP_1520 0.17 -0.1 1,726,230 + PP_1520 0.22 -1.3 1,726,231 - PP_1520 0.22 +0.9 1,726,231 - PP_1520 0.22 -0.6 1,726,231 - PP_1520 0.22 +0.5 1,726,231 - PP_1520 0.22 +0.5 1,726,326 + +3.4 1,726,326 + PP_1520 0.26 -0.4 1,726,327 - PP_1520 0.26 +0.7 1,726,329 + PP_1520 0.26 -0.1 1,726,330 - PP_1520 0.26 +0.5 1,726,330 - PP_1520 0.26 -0.9 1,726,498 - PP_1520 0.33 -0.3
Or see this region's nucleotide sequence