Strain Fitness in Pseudomonas putida KT2440 around PP_1458

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
1,662,104	+		PP_1457	0.20	-1.5
1,662,104	+		PP_1457	0.20	-0.6
1,662,104	+		PP_1457	0.20	-0.6
1,662,104	+		PP_1457	0.20	-0.6
1,662,104	+		PP_1457	0.20	-0.3
1,662,105	-		PP_1457	0.20	-1.4
1,662,105	-		PP_1457	0.20	+0.7
1,662,267	-		PP_1457	0.33	+0.2
1,662,284	+		PP_1457	0.35	+0.4
1,662,284	+		PP_1457	0.35	+0.1
1,662,284	+		PP_1457	0.35	-0.5
1,662,284	+		PP_1457	0.35	+0.6
1,662,284	+				+1.9
1,662,284	+		PP_1457	0.35	-0.1
1,662,293	+		PP_1457	0.36	-1.0
1,662,411	-		PP_1457	0.46	-0.6
1,662,411	-		PP_1457	0.46	-1.1
1,662,476	+		PP_1457	0.51	-0.4
1,663,094	-				+0.8
1,663,103	+				+0.7
1,663,103	+				+1.2
1,663,166	-				+0.5
1,663,254	-		PP_1458	0.12	-1.7
1,663,254	-		PP_1458	0.12	-0.4
1,663,255	+		PP_1458	0.13	+1.9
1,663,255	+		PP_1458	0.13	-0.6
1,663,274	+		PP_1458	0.14	-1.6
1,663,275	-		PP_1458	0.14	-0.4
1,663,276	+		PP_1458	0.14	-1.1
1,663,277	-		PP_1458	0.14	-1.3
1,663,413	-		PP_1458	0.25	-1.6
1,663,425	+		PP_1458	0.26	+0.2
1,663,559	-		PP_1458	0.36	-0.8
1,663,579	+		PP_1458	0.37	+0.7
1,663,579	+		PP_1458	0.37	-0.0
1,663,580	-		PP_1458	0.38	+0.4
1,663,580	-		PP_1458	0.38	-2.2
1,663,580	-		PP_1458	0.38	+0.0
1,663,607	-		PP_1458	0.40	-0.0
1,663,618	+		PP_1458	0.40	-1.8
1,663,664	-		PP_1458	0.44	+1.2
1,663,759	+		PP_1458	0.51	+0.0
1,663,759	+		PP_1458	0.51	-3.5
1,663,759	+		PP_1458	0.51	-0.1
1,663,759	+		PP_1458	0.51	-1.0
1,663,760	-		PP_1458	0.51	-2.1
1,663,760	-		PP_1458	0.51	-0.4
1,663,760	-		PP_1458	0.51	-0.1
1,663,767	+		PP_1458	0.52	+0.1
1,663,767	+		PP_1458	0.52	-0.8
1,663,768	-		PP_1458	0.52	-0.7
1,664,050	+		PP_1458	0.74	-1.3
1,664,051	-		PP_1458	0.74	-1.7
1,664,051	-		PP_1458	0.74	-0.6
1,664,083	-		PP_1458	0.76	-1.6
1,664,083	-		PP_1458	0.76	+0.5
1,664,130	-		PP_1458	0.80	+0.2
1,664,130	-		PP_1458	0.80	-0.8
1,664,278	+				-0.8
1,664,279	-				-0.3
1,664,279	-				-1.6
1,664,279	-				-0.5
1,664,285	-				-2.1
1,664,285	-				-0.7
1,664,293	+				+2.1
1,664,293	+				-1.0
1,664,374	+				-0.6
1,664,374	+				+0.6
1,664,375	-				+0.3
1,664,515	-				+0.2
1,664,515	-				-0.5
1,664,657	+				-1.3
1,664,657	+				-1.8
1,664,658	-				+0.2
1,664,659	+				-0.5
1,664,659	+				+0.8
1,664,659	+				-2.0
1,664,659	+				+0.3
1,664,659	+				-1.6
1,664,659	+				-0.1
1,664,659	+				-1.1
1,664,659	+				-1.2
1,664,659	+				-1.7
1,664,659	+				-0.7
1,664,659	+				+0.9
1,664,659	+				-0.9
1,664,660	-				-1.4
1,664,660	-				+0.4
1,664,660	-				-0.3
1,664,660	-				+0.8
1,664,660	-				-0.0
1,664,660	-				-0.4
1,664,660	-				-0.7
1,664,663	+				-0.6
1,664,740	+				-1.1
1,664,889	+		PP_1459	0.19	+1.2
1,664,993	-				+1.4
1,665,090	-		PP_1459	0.35	-1.3
1,665,090	-		PP_1459	0.35	+3.2
1,665,098	+		PP_1459	0.36	-0.7
1,665,098	+		PP_1459	0.36	+1.1
1,665,099	-		PP_1459	0.36	-1.3
1,665,204	-		PP_1459	0.45	+0.3
1,665,204	-		PP_1459	0.45	-0.1