Strain Fitness in Pseudomonas putida KT2440 around PP_1262

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
1,442,824	+		PP_1261	0.75	-0.3
1,442,825	-		PP_1261	0.76	+1.1
1,442,825	-		PP_1261	0.76	+0.2
1,442,834	-		PP_1261	0.77	+0.4
1,443,127	-				+0.6
1,443,148	+				-0.1
1,443,149	-				-0.2
1,443,205	+				-0.3
1,443,206	-				+0.9
1,443,351	-		PP_1262	0.12	+0.2
1,443,366	-		PP_1262	0.14	+0.4
1,443,374	+		PP_1262	0.14	+0.1
1,443,374	+		PP_1262	0.14	+1.3
1,443,374	+		PP_1262	0.14	+0.1
1,443,374	+		PP_1262	0.14	+0.3
1,443,375	-		PP_1262	0.14	+0.7
1,443,375	-		PP_1262	0.14	+0.4
1,443,409	+		PP_1262	0.18	-0.9
1,443,410	-		PP_1262	0.18	+0.8
1,443,424	-		PP_1262	0.20	-0.4
1,443,452	+		PP_1262	0.23	-1.3
1,443,452	+		PP_1262	0.23	+1.1
1,443,452	+		PP_1262	0.23	+0.6
1,443,452	+		PP_1262	0.23	+1.1
1,443,671	+		PP_1262	0.46	-0.1
1,443,698	+		PP_1262	0.49	-0.4
1,443,698	+		PP_1262	0.49	-0.7
1,443,699	-		PP_1262	0.49	+1.7
1,443,699	-		PP_1262	0.49	-0.0
1,443,699	-		PP_1262	0.49	+0.7
1,443,771	-		PP_1262	0.56	+1.0
1,443,962	+		PP_1262	0.76	-0.4
1,443,966	+		PP_1262	0.77	+0.6
1,443,966	+		PP_1262	0.77	+0.2
1,443,966	+		PP_1262	0.77	+0.1
1,443,966	+		PP_1262	0.77	+0.2
1,443,967	-		PP_1262	0.77	+0.4
1,443,992	+		PP_1262	0.80	-0.1
1,444,033	-		PP_1262	0.84	+0.8
1,444,148	-				-0.5
1,444,201	+				-0.2
1,444,227	-				-1.7
1,444,269	+				-1.2
1,444,310	+				-0.0
1,444,332	+				+0.6
1,444,496	+				+0.1
1,444,496	+				-0.3
1,444,496	+				+0.5
1,444,496	+				+0.3
1,444,497	-				-0.0
1,444,497	-				-1.4
1,444,573	-		PP_1263	0.13	-0.7
1,444,755	+		PP_1263	0.25	-0.8
1,444,755	+		PP_1263	0.25	+0.0
1,444,755	+		PP_1263	0.25	+0.2
1,444,755	+		PP_1263	0.25	+0.8
1,444,756	-		PP_1263	0.25	+0.5
1,444,818	+		PP_1263	0.29	+0.4
1,444,941	+		PP_1263	0.37	-0.2
1,444,941	+		PP_1263	0.37	-0.0
1,444,941	+		PP_1263	0.37	-1.3
1,444,942	-				+2.0