Strain Fitness in Pseudomonas putida KT2440 around PP_1099
Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
| Position | Strand | Gene | LocusTag | Fraction | 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. |
|---|---|---|---|---|---|
| remove | |||||
| 1,256,795 | - | -0.2 | |||
| 1,256,853 | - | -0.5 | |||
| 1,256,904 | + | +2.4 | |||
| 1,256,904 | + | +4.0 | |||
| 1,256,912 | + | +2.8 | |||
| 1,256,913 | - | -0.1 | |||
| 1,256,981 | - | PP_1099 | 0.29 | -1.2 | |
| 1,257,103 | + | PP_1099 | 0.87 | -0.5 | |
| 1,257,104 | - | PP_1099 | 0.87 | -0.6 | |
| 1,257,104 | - | PP_1099 | 0.87 | -0.6 | |
| 1,257,104 | - | PP_1099 | 0.87 | +4.0 | |
| 1,257,243 | - | +0.3 | |||
| 1,257,388 | - | +2.4 | |||
| 1,257,411 | + | -0.7 | |||
| 1,257,411 | + | -0.2 | |||
| 1,257,584 | + | +4.0 | |||
| 1,257,591 | - | PP_1100 | 0.23 | +4.0 | |
| 1,257,591 | - | +4.0 | |||
| 1,257,747 | - | PP_1100 | 0.51 | +1.7 | |
| 1,257,797 | + | +4.0 | |||
| 1,257,966 | - | +4.0 | |||
| 1,257,987 | - | +3.0 | |||
| 1,258,072 | + | -0.4 |
Or see this region's nucleotide sequence