Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_1026 and PP_1027 are separated by 3 nucleotides PP_1027 and PP_1028 are separated by 94 nucleotides
PP_1026: PP_1026 - Peptidase, M23/M37 family, at 1,170,842 to 1,171,669
_1026
PP_1027: PP_1027 - Exodeoxyribonuclease 7 large subunit, at 1,171,673 to 1,173,052
_1027
PP_1028: PP_1028 - Transcriptional regulator, LysR family, at 1,173,147 to 1,174,025
_1028
Position (kb) 1171
1172
1173
1174 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1170.771 kb on + strand at 1170.772 kb on - strand at 1170.772 kb on - strand at 1170.772 kb on - strand at 1170.772 kb on - strand at 1170.799 kb on + strand at 1171.025 kb on - strand, within PP_1026 at 1171.072 kb on - strand, within PP_1026 at 1171.371 kb on + strand, within PP_1026 at 1171.385 kb on + strand, within PP_1026 at 1171.599 kb on + strand at 1171.599 kb on + strand at 1171.600 kb on - strand at 1171.682 kb on + strand at 1171.683 kb on - strand at 1172.507 kb on + strand, within PP_1027 at 1172.515 kb on + strand, within PP_1027 at 1172.516 kb on - strand, within PP_1027 at 1172.519 kb on + strand, within PP_1027 at 1172.520 kb on - strand, within PP_1027 at 1172.743 kb on + strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.744 kb on - strand, within PP_1027 at 1172.751 kb on + strand, within PP_1027 at 1172.752 kb on - strand, within PP_1027 at 1172.892 kb on + strand, within PP_1027 at 1172.892 kb on + strand, within PP_1027 at 1172.893 kb on - strand, within PP_1027 at 1172.893 kb on - strand, within PP_1027 at 1172.893 kb on - strand, within PP_1027 at 1173.005 kb on - strand at 1173.064 kb on + strand at 1173.064 kb on + strand at 1173.064 kb on + strand at 1173.065 kb on - strand at 1173.065 kb on - strand at 1173.099 kb on + strand at 1173.315 kb on + strand, within PP_1028 at 1173.316 kb on - strand, within PP_1028 at 1173.403 kb on + strand, within PP_1028 at 1173.403 kb on + strand, within PP_1028 at 1173.404 kb on - strand, within PP_1028 at 1173.682 kb on + strand, within PP_1028 at 1173.946 kb on + strand at 1173.946 kb on + strand at 1173.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 1,170,771 + -1.7 1,170,772 - -0.8 1,170,772 - -0.6 1,170,772 - +0.8 1,170,772 - +3.0 1,170,799 + +0.7 1,171,025 - PP_1026 0.22 +1.0 1,171,072 - PP_1026 0.28 +0.0 1,171,371 + PP_1026 0.64 +0.4 1,171,385 + PP_1026 0.66 -1.0 1,171,599 + +0.5 1,171,599 + -0.1 1,171,600 - -0.5 1,171,682 + +1.2 1,171,683 - +0.2 1,172,507 + PP_1027 0.60 +1.2 1,172,515 + PP_1027 0.61 -0.7 1,172,516 - PP_1027 0.61 +1.4 1,172,519 + PP_1027 0.61 -1.1 1,172,520 - PP_1027 0.61 -0.5 1,172,743 + PP_1027 0.78 -0.4 1,172,744 - PP_1027 0.78 +0.3 1,172,744 - PP_1027 0.78 +1.0 1,172,744 - PP_1027 0.78 +1.2 1,172,744 - PP_1027 0.78 -1.0 1,172,744 - PP_1027 0.78 -1.8 1,172,751 + PP_1027 0.78 -0.4 1,172,752 - PP_1027 0.78 -0.4 1,172,892 + PP_1027 0.88 -1.4 1,172,892 + PP_1027 0.88 -2.3 1,172,893 - PP_1027 0.88 +0.3 1,172,893 - PP_1027 0.88 +1.2 1,172,893 - PP_1027 0.88 +0.1 1,173,005 - -0.9 1,173,064 + -0.2 1,173,064 + +0.8 1,173,064 + -0.3 1,173,065 - -0.6 1,173,065 - -0.9 1,173,099 + -0.3 1,173,315 + PP_1028 0.19 +3.2 1,173,316 - PP_1028 0.19 -0.0 1,173,403 + PP_1028 0.29 +0.5 1,173,403 + PP_1028 0.29 -0.4 1,173,404 - PP_1028 0.29 -1.4 1,173,682 + PP_1028 0.61 +0.3 1,173,946 + +0.1 1,173,946 + +2.6 1,173,947 - +0.6
Or see this region's nucleotide sequence